Comprehensive integrative analysis of circadian rhythms in human saliva

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: May 10, 2025

Abstract The circadian clock orchestrates vital physiological functions, with its dysregulation implicated in various pathologies. Assessing human status via the measurement of rhythms is crucial for health management and disease treatment. Saliva provides a non-invasive means such analysis. In this study, we examined related parameters 21 healthy individuals ( n = 4 – 19 per experiment), assessing different data types, including saliva gene expression our TimeTeller ® methodology, hormone levels, cell composition, self-assessment tests chronotype evaluation. While substantial individual variability profiles was observed, found significant correlations between acrophases ARNTL1 cortisol, both correlated bedtime on sampling day. Our findings validate robustness reliability method determining peripheral humans, offering potential clinical applications diverse cohorts.

Language: Английский

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Recent omics advances in hair aging biology and hair biomarkers analysis

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 91, P. 102041 - 102041

Published: Aug. 25, 2023

Language: Английский

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Overview of the Circadian Clock in the Hair Follicle Cycle

Biomolecules, Journal Year: 2023, Volume and Issue: 13(7), P. 1068 - 1068

Published: July 3, 2023

The circadian clock adapts to the light–dark cycle and autonomously generates physiological metabolic rhythmicity. Its activity depends on central suprachiasmatic pacemaker. However, it also has an independent function in peripheral tissues such as liver, adipose tissue, skin, which integrate environmental signals energy homeostasis. Hair follicles (HFs) maintain homeostasis through HF cycle, heavily stem cell self-renewal related reprogramming. Studies have shown that dysregulation HFs perturbs progression. Moreover, there is increasing evidence exerts a significant influence glucose metabolism, feeding/fasting, differentiation, senescence. This suggests crosstalk plays essential role regulating regeneration. An improved understanding of may facilitate discovery new drug targets for hair loss. Therefore, present review provides discussion relationship between regeneration, mainly from perspective summarizes current mechanisms by function.

Language: Английский

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Interplay of Light, Melatonin, and Circadian Genes in Skin Pigmentation Regulation

Pigment Cell & Melanoma Research, Journal Year: 2025, Volume and Issue: 38(1)

Published: Jan. 1, 2025

Circadian regulation of skin pigmentation is essential for thermoregulation, ultraviolet (UV) protection, and synchronization cell renewal. This involves both cell-autonomous photic responses non-cell-autonomous hormonal control, particularly through melatonin produced in a light-sensitive manner. Photosensitive opsins, cryptochromes, regulate circadian rhythms pigment cells. We studied light/dark cycles coordination melanin synthesis proliferation Xenopus laevis melanophores. In vivo, tadpole shows robust mainly hormone-driven, that isolated melanophores respond strongly to but only slightly light. Melanophore faster the dark slower with as compared 12/12 cycle. Expression core genes (clock, bmal1, per1, per2, per3, cry1, cry2, cry4) melatonin-treated cells during light phase mimics expression. Overexpression individual Crys did not affect melanization or proliferation, likely due their cooperative actions. Melanin was inhibited by cycle deregulation (a) pharmacological inhibition Cry1 Cry2 degradation KL001, (b) continuous conditions, (c) treatment. Our findings suggest regulation, rather than proliferative capacity, alters

Language: Английский

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Identification of lncRNAs involved in the hair follicle cycle transition of cashmere goats in response to photoperiod change

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: May 15, 2025

Cashmere goats, as one of the important domesticated animal species, are known for their high-quality fiber. The growth cashmere has seasonal variations caused by photoperiodic changes, but molecular genetic mechanisms underlying this phenotype including functional role long non-coding RNAs (lncRNA) is still poorly understood. In study, we analyzed RNA-seq dataset 39 goat skin samples all different stages and identified 1591 lncRNAs. These lncRNAs exhibited stage-specific expression patterns. Combining shortened light hair follicle cycles, found that 68% differentially photo-responsive showed similar trends during transition phase I (early anagen to phase). This suggests mechanism light-controlled induction follicles from early I. According weighted gene co-expression network analyses (WGCNA) analysis, it was two clusters 10 hub participated in transformation cycle, inducing enter full advance. may regulate development cycle through cis- or trans-regulation on clock genes, SLC superfamily fibroblast factor genes. study key target genes probably participating cycle. will help further elucidate development.

Language: Английский

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Autophagy Dysfunction: The Kernel of Hair Loss?

Clinical Cosmetic and Investigational Dermatology, Journal Year: 2024, Volume and Issue: Volume 17, P. 1165 - 1181

Published: May 1, 2024

Autophagy is recognized as a crucial regulatory process, instrumental in the removal of senescent, dysfunctional, and damaged cells. Within autophagic lysosomal digestion plays critical role elimination impaired organelles, thus preserving fundamental cellular metabolic functions various biological processes. Mitophagy, targeted process that specifically focuses on mitochondria, essential for sustaining health energy balance. Therefore, deep comprehension operational mechanisms implications autophagy mitophagy vital disease prevention treatment. In this context, we examine during hair follicle cycles, closely scrutinizing their potential association with loss. We also conduct thorough review behind mitophagy, highlighting interaction stem cells dermal papilla conclusion, investigate manipulating pathways to develop innovative therapeutic strategies

Language: Английский

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Optimised Stable Lighting Strengthens Circadian Clock Gene Rhythmicity in Equine Hair Follicles

Animals, Journal Year: 2023, Volume and Issue: 13(14), P. 2335 - 2335

Published: July 17, 2023

Hair follicles (HF) represent a useful tissue for monitoring the circadian clock in mammals. Irregular light exposure causes disruption and represents welfare concern stabled horses. We aimed to evaluate impact of two stable lighting regimes on gene rhythmicity HF from racehorses. Two groups five Thoroughbred racehorses training at commercial racehorse yard were exposed standard incandescent or customized LED system. The control group received bulbs used according practice. treatment timed, blue-enriched white by day dim red night. On weeks 0 20, mane hairs collected 4 h intervals 24 h. Samples stored RNAlater -20 °C. RNA was isolated samples interrogated quantitative PCR core genes: ARNTL, CRY1, PER1, PER2, NR1D2, clock-controlled DBP. Cosinor analyses revealed NR1D2 PER2 approached significance CRY1 (p = 0.013, p 0.051, respectively) week 20 only. No detected Results suggest that practices stables may be improved better stimulate optimum functioning

Language: Английский

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The rhythms of histones in regeneration: The epigenetic modifications determined by clock genes

Experimental Dermatology, Journal Year: 2024, Volume and Issue: 33(1)

Published: Jan. 1, 2024

Abstract The evolutionary establishment of an internal biological clock is a primordial event tightly associated with 24‐h period. Changes in the circadian rhythm can affect cellular functions, including proliferation, DNA repair and redox state. Even isolated organs, tissues cells maintain autonomous rhythm. These cell‐autonomous molecular mechanisms are driven by intracellular genes, such as BMAL1. Little known about role core genes epigenetic modifications skin. Our focus was to identify BMAL1‐driven gene transcription mapping acetylation landscape histones epithelial responding injury. We explored BMAL1 epidermal wound tissue regeneration using loss‐of‐function approach vivo. worked knockout mice contraction‐resistance healing protocol, determining histone specific antibodies detect levels H3 H4. found significant differences both homeostatic injured skin deregulated intact displayed varied H4, hyperacetylation Lys 9 (H3K9). most pronounced changes were observed at site, notable alterations pattern findings reveal importance response injury indicate that modulation its events could be therapeutically harnessed improve regeneration.

Language: Английский

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The role of telomerase in hair growth and relevant disorders: A review

Journal of Cosmetic Dermatology, Journal Year: 2023, Volume and Issue: 22(11), P. 2925 - 2929

Published: Sept. 4, 2023

Abstract Background Hair diseases may present with hair loss, hirsutism, melanin abnormalities and other manifestations. follicles are known as mini‐organs that undergo periodic remodeling, their constant regeneration in vivo reflects interesting anti‐aging functions. Telomerase prevents cellular senescence by maintaining telomere length, but its excessive proliferation cancer cells also induce cancer. However, the effects of telomerase growth have rarely been reported. Methods In this study, we reviewed role disorders through literature search analysis. Results There is growing evidence plays an important follicle function proliferation. Changes levels found a variety disorders. Conclusion positive expected to become new target for treatment alopecia or future.

Language: Английский

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Integumentary System

Royal Society of Chemistry eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 374 - 396

Published: Feb. 23, 2024

Skin is a complex organ, consisting of several layers, each containing different kinds cells performing functions, including defense mechanisms against pathogens, chemicals, UV light, and mechanical injury, hydration, temperature regulation, sebum production, skin blood flow barrier function. regulated by the central clock, while also harbor their own molecular circuitry, with cell biochemical oscillator interlocked transcription–translation feedback loops. After brief overview structure organization, illustrative examples 24-hour variations in some functions are presented. The involvement circadian clock playing key roles affecting expression distinct gene sets physiological then reviewed. implication machinery present further discussed relation to various diseases, ranging from cancer infections, inflammatory wound healing. To conclude, thoughts on strategies maintain health counteract disease conditions offered, opening vast field for future investigation.

Language: Английский

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