Animal Models of Hypertension (ISIAH Rats), Catatonia (GC Rats), and Audiogenic Epilepsy (PM Rats) Developed by Breeding

Biomedicines, Journal Year: 2023, Volume and Issue: 11(7), P. 1814 - 1814

Published: June 24, 2023

Research into genetic and physiological mechanisms of widespread disorders such as arterial hypertension well neuropsychiatric other human diseases is urgently needed in academic practical medicine the field biology. Nevertheless, studies have many limitations pose difficulties that can be overcome by using animal models. To date, for purposes creating models pathologies, several approaches been used: pharmacological/chemical intervention; surgical procedures; technologies transgenic animals, knockouts, or knockdowns; breeding. Although some these are good certain research aims, they drawbacks, greatest being a strong perturbation (in biological system) that, along with expected effect, exerts side effects study. Therefore, investigating pathogenesis disease, obtained selection target trait high value this approach allows creation model “natural” manifestation pathology. In review, three rat described: ISIAH rats (arterial hypertension), GC (catatonia), PM (audiogenic epilepsy), which developed breeding Laboratory Evolutionary Genetics at Institute Cytology (the Siberian Branch Russian Academy Sciences).

Language: Английский

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Candidate SNP Markers Significantly Altering the Affinity of TATA-Binding Protein for the Promoters of Human Hub Genes for Atherogenesis, Atherosclerosis and Atheroprotection

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(10), P. 9010 - 9010

Published: May 19, 2023

Atherosclerosis is a systemic disease in which focal lesions arteries promote the build-up of lipoproteins and cholesterol they are transporting. The development atheroma (atherogenesis) narrows blood vessels, reduces supply leads to cardiovascular diseases. According World Health Organization (WHO), diseases leading cause death, has been especially boosted since COVID-19 pandemic. There variety contributors atherosclerosis, including lifestyle factors genetic predisposition. Antioxidant diets recreational exercises act as atheroprotectors can retard atherogenesis. search for molecular markers atherogenesis atheroprotection predictive, preventive personalized medicine appears be most promising direction study atherosclerosis. In this work, we have analyzed 1068 human genes associated with atherogenesis, atherosclerosis atheroprotection. hub regulating these processes found ancient. silico analysis all 5112 SNPs their promoters revealed 330 candidate SNP markers, statistically significantly change affinity TATA-binding protein (TBP) promoters. These made us confident that natural selection acts against underexpression At same time, upregulation one promotes health.

Language: Английский

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RatDEGdb: a knowledge base of differentially expressed genes in the rat as a model object in biomedical research

Vavilov Journal of Genetics and Breeding, Journal Year: 2023, Volume and Issue: 27(7), P. 794 - 806

Published: Dec. 11, 2023

The animal models used in biomedical research cover virtually every human disease. RatDEGdb, a knowledge base of the differentially expressed genes (DEGs) rat as model object is collection published data on gene expression strains simulating arterial hypertension, age-related diseases, psychopathological conditions and other afflictions. current release contains information 25,101 DEGs representing 14,320 unique that change transcription levels 21 tissues 10 genetic 11 diseases based 45 original scientific papers. RatDEGdb novel that, unlike any database, it offers manually curated annotations rats with use independent clinical equal changes homologous revealed people pathologies. put were annotated their homologs affected people. In its release, 94,873 such for 321 836 959 papers found PubMed. may be interesting first all to geneticists, molecular biologists, physicians, advisors well experts biopharmaceutics, bioinformatics personalized genomics. publicly available at https://www.sysbio.ru/RatDEGdb.

Language: Английский

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A Principal Components Analysis and Functional Annotation of Differentially Expressed Genes in Brain Regions of Gray Rats Selected for Tame or Aggressive Behavior

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4613 - 4613

Published: April 23, 2024

The process of domestication, despite its short duration as it compared with the time scale natural evolutionary process, has caused rapid and substantial changes in phenotype domestic animal species. Nonetheless, genetic mechanisms underlying these remain poorly understood. present study deals an analysis transcriptomes from four brain regions gray rats (Rattus norvegicus), serving experimental model object domestication. We gene expression profiles hypothalamus, hippocampus, periaqueductal matter, midbrain tegmental region between tame domesticated aggressive revealed subdivisions differentially expressed genes by principal components that explain main part variance. Functional (in DAVID (Database for Annotation, Visualization Integrated Discovery) Bioinformatics Resources database) allowed us to identify describe key biological processes can participate formation different behavioral patterns seen two groups rats. Using STRING- DB (search tool recurring instances neighboring genes) web service, we built a association network. engaged broad network interactions have been identified. Our offers data on whose levels change response artificial selection behavior during

Language: Английский

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Candidate SNP Markers Significantly Altering the Affinity of the TATA-Binding Protein for the Promoters of Human Genes Associated with Primary Open-Angle Glaucoma

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12802 - 12802

Published: Nov. 28, 2024

Primary open-angle glaucoma (POAG) is the most common form of glaucoma. This condition leads to optic nerve degeneration and eventually blindness. Tobacco smoking, alcohol consumption, fast-food diets, obesity, heavy weight lifting, high-intensity physical exercises, many other bad habits are lifestyle-related risk factors for POAG. By contrast, moderate-intensity aerobic exercise Mediterranean diet can alleviate In this work, we first time estimated phylostratigraphic age indices (PAIs) all 153 POAG-related human genes in NCBI Gene Database. allowed us separate them into two groups: that appeared before after phylum Chordata, is, ophthalmologically speaking, camera-type eye evolved. Next, genes’ promoters, silico predicted 3835 candidate SNP markers significantly change TATA-binding protein (TBP) affinity these promoters and, through molecular mechanism, expression levels genes. Finally, verified our results against five independent web services—PANTHER, DAVID, STRING, MetaScape, GeneMANIA—as well as ClinVar database. It was concluded POAG likely be a symptom self-domestication syndrome, downside being civilized.

Language: Английский

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