Semen Cryopreservation to Expand Male Fertility in Cancer Patients: Intracase Evaluation of Semen Quality DOI Open Access
G. Peluso, Veronica Tisato, Ajay Vikram Singh

et al.

Journal of Personalized Medicine, Journal Year: 2023, Volume and Issue: 13(12), P. 1654 - 1654

Published: Nov. 27, 2023

To preserve male fertility after diagnosis of any kind cancer, a prompt assessment the semen quality and an appropriate cryopreservation must be performed before radio-chemotherapy starts. The present work aims to evaluate parameters at different cancer patients thawing. Testicular tumors lymphomas are among most common cancers in younger patients, while chemotherapy significantly increases patients' survival, it can epigenetically alter fluid, resulting temporary or permanent infertility. We analyzed data from database Gamete Cryopreservation Center (Annunziata Hospital, CS; Italy) period 2011-2020 cohort 254 aged 18-56 years. evaluation was blind manner anonymously recovered; main referring were assessed accordance with WHO guidelines decision limits (6th edition; 2021). types as follows: testis (TC; n = 135; 53.1%), hematological (HC; 76; 29.9%), other (OC; 43; 17%). Comparing TC vs. HC (P1) OC (P2), had worst quality: sperm number/mL (P1 0.0014; P2 0.004), total motility 0.02; 0.07), progressive 0.04; 0.05), viability 0.01; 0.02), percentage atypical morphology 0.05; 0.03). After thawing, recovery lowered, accounting for 46.82% 16.75%, respectively, whole cohort; similarly, subgroups ascribed TC, they showed lowest recovery. Strong correlation existed between pre- post-cryopreservation (p < 0.001) subgroup 0.05). All subgroups, extents, findings below reference values, suggesting diverse susceptibilities cryodamage. Cancer associated treatments affect quality, meaning should considered useful personalized prerogative timely manner.

Language: Английский

The Effect of Edaravone Supplementation Prior to Cryopreservation on Sperm Parameters, DNA Integrity, Apoptosis, Lipid Peroxidation, and Mitochondrial Membrane Potential in Infertile Men with Asthenoteratozoospermia DOI
Maryam Ghasemi, Hamid Nazarian, Zahra Shams Mofarahe

et al.

Reproductive Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

1
Effects of Slow Freezing and Vitrification of Human Semen on Post-Thaw Semen Quality and miRNA Expression DOI Open Access
Rebeka Podgrajšek, Luka Bolha,

Tjaša Pungert

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4157 - 4157

Published: April 9, 2024

Semen cryopreservation has played an important role in medically assisted reproduction for decades. In addition to preserving male fertility, it is sometimes used overcoming logistical issues. Despite its proven clinical usability and safety, there a lack of knowledge how affects spermatozoa at the molecular level, especially terms non-coding RNAs. Therefore, we conducted this study, where compared slow freezing vitrification good- poor-quality human semen samples by analyzing conventional sperm quality parameters, performing functional tests expression miRNAs. The results revealed that normozoospermic does not alter maturity (protamine staining, hyaluronan binding), although can increase DNA fragmentation lower motility. On both types cryopreservation, miRNAs from are significantly overexpressed those native patients, but oligozoospermic samples, effect observed only after vitrification. Moreover, show selected mostly samples. Conversely, when vitrified were compared, determined miR-99b-5p was comparing freezing, miR-15b-5p miR-34b-3p under-expressed our imply modulate miRNA profiles become comparable

Language: Английский

Citations

4
The application of mean number of DNA breakpoints in sperm cryopreservation DOI

Bei Yan,

Juan Wang, Yue Zhou

et al.

Cryobiology, Journal Year: 2024, Volume and Issue: 116, P. 104937 - 104937

Published: July 8, 2024

Language: Английский

Citations

4
Effects of Essential Oils as Antioxidant and Cryoprotective Agents in Improving Frozen and Thawed Human Sperm Criteria DOI Creative Commons
Hamza Goujet, Ismail Kaarouch,

Abderrahim Malki

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 75 - 75

Published: Jan. 10, 2025

Sperm cryopreservation provides patients undergoing oncological, surgical, or infertility treatments the opportunity to conceive their own children, using assisted reproductive technologies. However, freezing-thawing process can negatively influence both quantity and quality of spermatozoa, mainly due an excessive production reactive oxygen species and/or impaired antioxidant defense system in sperm. Aromatic medicinal plants synthesize essential oils with proprieties as a part ecological adaptation environmental stress, thanks rich bioactive phytochemical components. This study aimed assess sperm progressive motility, viability, plasma membrane functionality, lipid peroxidation levels human cryopreserved normozoospermic (n = 51) asthenozoospermic samples without addition Thymus satureoides (TSEO) (20 µg/mL), Artemisia vulgaris (AVEO) (48 Rosmarinus officinalis (ROEO) (13 µg/mL) oils. parameters were significantly better preserved ROEO (p < 0.05) 0.01). In contrast, TSEO had negative impact for groups 0.05). Meanwhile, no significant effects observed AVEO. summary, revealed that vitro agents during be either beneficial, which helps preserve fertilizing potential, detrimental spermicidal agents.

Language: Английский

Citations

0
Fertility Preservation in More than 7000 Male Patients: A Single-Center, Tertiary Care Registry Study over 30 Years DOI Open Access
Andrea Graziani, Giuseppe Grande, Michel Martin

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(4), P. 689 - 689

Published: Feb. 18, 2025

Background/Objectives: Semen cryopreservation has been widely used in recent decades, mainly patients with male factor infertility (MFI) and cancer (to preserve their fertility potential before undergoing gonadotoxic treatments, such as chemotherapy or radiotherapy). Methods: we evaluated the temporal trend existence of factors determining usage cryopreserved semen (in order, for instance, to conceive using assisted reproduction techniques (ARTs)). We 7044 who since 1991, evaluating clinical information diagnosis, age, method which collection took place number pick-ups use ART. Results: The mean age was 33.12 ± 8.41 years. most common cancers were testicular (1758/3262) hematological (942/3262). Patients underwent MFI picked up samples much more than neoplastic (42.15% vs. 8.55%). 35-40 years old frequently when compared other groups. Moreover, found several data regarding sub-types methods collection. Conclusions: According our results, indication cryopreservation, might represent a simple way predict future

Language: Английский

Citations

0
The role and implication of platelet‐rich plasma in male factor infertility: A systematic review of human studies DOI Creative Commons
Karl H. Pang

Andrology, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Background Cryopreservation causes sperm injury and the success of surgical retrieval (SSR) for azoospermic men is just over half depending on cause azoospermia. The role autologous platelet‐rich plasma (PRP) in male factor infertility (MFI) unclear. Objective To conduct a systematic review PRP MFI focusing human studies. Methods A was conducted using PubMed with reference to PRISMA 2020 statement. risk bias assessment included studies performed JBI checklists. Outcome measures effects cryopreservation, semen parameters, SSR. Results search retrieved 119 articles 10 met pre‐defined PICO criteria. These 7 prospective studies, 1 randomised‐controlled study, retrospective case report. appeared improve decrease DNA fragmentation, recovery cryopreserved sperm, SSR rate. report demonstrated successful intracytoplasmic injection pregnancy following incubation patient previous failed IVF attempts. Discussion conclusion This first summarising data from MFI. inclusion criteria outcomes across varied, limiting ability quantitative analysis. Data early are promising. However, there lack well‐designed clinical this topic, further research needed replicate current findings evaluate potential benefits

Language: Английский

Citations

0
Assessment of sperm DNA integrity and ICSI outcome after sperm cryopreservation DOI Creative Commons
Eman Mohamed,

S.A. Abd- Elmaksoud,

Tharwat G. Abdelkader

et al.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Journal Year: 2025, Volume and Issue: 77(3)

Published: Jan. 1, 2025

ABSTRACT The association between suboptimal sperm parameters and DNA damage in mature spermatozoa indicates that some individuals may have intrinsic issues with spermatogenesis. objective of this study is to evaluate the impact cryopreservation reactive oxygen species (ROS) on stability, as assessed by Sperm Chromatin Structure Assay (SCSA) motility viability, ICSI Result. Participants contacted Fertility Clinic provided written informed consent. All data were subsequently handled processed a confidential anonymous manner. There was source 300 participants (600 semen samples) study, divided into 3 groups. female partners underwent ICSI, (some oocytes injected fresh sperms others cryopreserved for same patient (liquid nitrogen at−196°C, accordance conventional protocols). Cryopreservation from fertile men does not seem adversely affect integrity either or prepared samples. However, morphology both samples significantly compromised process. No evidence cryopreserving healthy males would compromise genetic material laboratory-prepared

Language: Английский

Citations

0
Probing the Size Effect of Graphene Oxide Nanosheets on Ice Crystal Regulation and Laser-Assisted Rapid Rewarming DOI

Liuyue Li,

Jixiang Zhang, Yi-Fang Li

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(26), P. 33149 - 33158

Published: June 18, 2024

Two-dimensional (2D) nanomaterials have attracted many researchers to explore the effect of ice control and rapid deicing due their functional groups, large specific surface area, excellent photothermal properties. However, impact size effects on crystal formation, growth, performance has been rarely explored. Here, graphene oxide nanosheets (GO NSs) with controllable sizes were used as a representative 2D probe regulation rewarming in cell cryopreservation. All GO NSs exhibited notable inhibitory during recrystallization process. Significantly, when was smaller than certain (<150 nm), they showed more significant suppression effects, which could reduce about 17% that pure water. Meanwhile, experiments also indicated smaller-sized better behavior, 90 nm (GO-90) heating 70 °C just 1 min induced by an 808 laser (2 W/cm

Language: Английский

Citations

3
The Role of microRNA in Spermatogenesis: Is There a Place for Fertility Preservation Innovation? DOI Open Access

Charlotte Klees,

Chrysanthi Alexandri,

Isabelle Demeestere

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 460 - 460

Published: Dec. 29, 2023

Oncological treatments have dramatically improved over the last decade, and as a result, survival rates for cancer patients also improved. Quality of life, including concerns about fertility, has become major focus both oncologists patients. While oncologic are often highly effective at suppressing neoplastic growth, they frequently associated with severe gonadotoxicity, leading to infertility. For male patients, therapeutic option preserve fertility is semen cryopreservation. In prepubertal immature testicular tissue can be sampled stored allow post-cure transplantation tissue, germ cells, or in vitro spermatogenesis. However, experimental techniques not yet been proven restoring sperm production these MicroRNAs (miRNAs) emerged promising molecular markers tools various diseases. These small regulatory RNAs possess unique characteristic having multiple gene targets. MiRNA-based therapeutics can, therefore, used modulate expression different genes involved signaling pathways dysregulated by changes physiological environment (disease, temperature, ex vivo culture, pharmacological agents). This review discusses possible role miRNA an innovative treatment preservation–restoration strategies describes diverse applications where new could serve protection agents.

Language: Английский

Citations

8
Sperm Chromatin Dispersion Test Detects Sperm DNA Fragmentation Mainly Associated with Unviable Spermatozoa and Underestimates the Values with Respect to TUNEL Assay DOI Open Access

Maria Emanuela Ragosta,

Giulia Traini,

Lara Tamburrino

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4481 - 4481

Published: April 19, 2024

Several clinical laboratories assess sperm DNA fragmentation (sDF) in addition to semen analysis male infertility diagnosis. Among tests evaluating sDF, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) and SCD (Sperm Chromatin Dispersion) are widely used. Our lab developed a modified version of (TUNEL/PI) able distinguish two populations (PI Brighter PI Dimmer) differently associated with viability reproductive outcomes. The aim this study was compare sDF levels detected by TUNEL/PI the samples from 71 subjects attending our Andrology Laboratory. results demonstrate that is less sensitive determining compared TUNEL/PI. statistically significant positive correlation found between evaluated Dimmer (consisting all dead spermatozoa) suggests mainly detects unviable spermatozoa. We confirmed most spermatozoa performing after incubation hypo-osmotic medium discriminate viable cells 52 samples. Such might explain lower ability test discriminating couples having successful ART outcomes demonstrated published metanalyses. Overall, indicate for diagnostic purposes.

Language: Английский

Citations

3