Journal of Personalized Medicine,
Journal Year:
2023,
Volume and Issue:
13(12), P. 1654 - 1654
Published: Nov. 27, 2023
To
preserve
male
fertility
after
diagnosis
of
any
kind
cancer,
a
prompt
assessment
the
semen
quality
and
an
appropriate
cryopreservation
must
be
performed
before
radio-chemotherapy
starts.
The
present
work
aims
to
evaluate
parameters
at
different
cancer
patients
thawing.
Testicular
tumors
lymphomas
are
among
most
common
cancers
in
younger
patients,
while
chemotherapy
significantly
increases
patients'
survival,
it
can
epigenetically
alter
fluid,
resulting
temporary
or
permanent
infertility.
We
analyzed
data
from
database
Gamete
Cryopreservation
Center
(Annunziata
Hospital,
CS;
Italy)
period
2011-2020
cohort
254
aged
18-56
years.
evaluation
was
blind
manner
anonymously
recovered;
main
referring
were
assessed
accordance
with
WHO
guidelines
decision
limits
(6th
edition;
2021).
types
as
follows:
testis
(TC;
n
=
135;
53.1%),
hematological
(HC;
76;
29.9%),
other
(OC;
43;
17%).
Comparing
TC
vs.
HC
(P1)
OC
(P2),
had
worst
quality:
sperm
number/mL
(P1
0.0014;
P2
0.004),
total
motility
0.02;
0.07),
progressive
0.04;
0.05),
viability
0.01;
0.02),
percentage
atypical
morphology
0.05;
0.03).
After
thawing,
recovery
lowered,
accounting
for
46.82%
16.75%,
respectively,
whole
cohort;
similarly,
subgroups
ascribed
TC,
they
showed
lowest
recovery.
Strong
correlation
existed
between
pre-
post-cryopreservation
(p
<
0.001)
subgroup
0.05).
All
subgroups,
extents,
findings
below
reference
values,
suggesting
diverse
susceptibilities
cryodamage.
Cancer
associated
treatments
affect
quality,
meaning
should
considered
useful
personalized
prerogative
timely
manner.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: May 29, 2024
Cryopreservation
of
sperm
can
cause
oxidative
stress
and
damage,
leading
to
decreased
different
functional
parameters
fertilization
potential.
In
this
study,
we
evaluated
two
types
H
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(16), P. 9112 - 9112
Published: Aug. 22, 2024
Semen
cryopreservation
results
in
the
differential
remodeling
of
molecules
presented
sperm,
and
these
alterations
related
to
reductions
sperm
quality
its
physiological
function
have
not
been
fully
understood.
Given
this,
this
study
aimed
investigate
cryoinjury
mechanism
goat
by
analyzing
changes
metabolic
characteristics
during
process.
The
ultra-high-performance
liquid
chromatography-quadrupole
time-of-flight
mass
spectrometry
(UHPLC-QTOF-MS)
technique
was
performed
explore
metabolite
profiles
fresh
(C
group),
equilibrated
(E
frozen-thawed
(F
group).
In
total,
2570
metabolites
positive
mode
2306
negative
were
identified,
respectively.
After
comparative
analyses
among
three
groups,
374
differentially
abundant
(DAMs)
C
vs.
E,
291
DAMs
F,
189
E
F
obtained
mode;
concurrently,
530
405
193
mode,
significantly
enriched
various
pathways,
including
31
pathways
25
28
Among
them,
65
across
comparisons
discovered,
which
may
be
tightly
associated
with
function.
Particularly,
functional
terms
such
as
TCA
cycle,
biosynthesis
unsaturated
fatty
acids,
sphingolipid
metabolism,
glycine,
serine
threonine
alpha-linolenic
acid
pyruvate
well
pivotal
like
ceramide,
betaine,
choline,
fumaric
acid,
L-malic
L-lactic
focused
on.
conclusion,
our
research
characterizes
composition
their
induced
process,
offering
a
critical
foundation
for
further
exploring
molecular
mechanisms
metabolism
influencing
freezing
tolerance
sperm.
Additionally,
impacts
equilibration
at
low
temperature
on
need
more
attentions
compared
thawing
Ovarian
tissue
cryopreservation
(OTC)
or
testicular
(TTC)
are
effective
and
often
the
only
options
for
fertility
preservation
in
female
male
patients
due
to
oncological,
medical,
social
aspects.
While
TTC
resumption
of
spermatogenesis,
either
vivo
vitro,
has
still
be
considered
an
experimental
approach
humans,
OTC
autotransplantation
been
applied
increasingly
preserve
with
more
than
200
live
births
worldwide.
However,
reproductive
cells
followed
by
gametogenesis,
may
interfere
sensitive
highly
regulated
cellular
processes.
In
particular,
epigenetic
profile,
which
includes
not
just
reversible
modifications
DNA
itself
but
also
post-translational
histone
modifications,
small
non-coding
RNAs,
gene
expression
availability,
storage
related
proteins
transcripts,
have
this
context.
Due
complex
reprogramming
maintenance
mechanisms
epigenome
germ
cells,
growing
embryos,
offspring,
carried
out
at
very
critical
moments
early
life
cycle.
Given
background,
safety
taking
into
account
profile
clarified.
Cryopreservation
mature
(including
Metaphase
II
oocytes
spermatozoa
collected
via
ejaculation
invasively
after
biopsy)
embryos
used
successfully
many
years
Medically
Assisted
Reproduction
(MAR).
freezing
vitro
gametogenesis
become
attractive
past,
while
few
human
studies
analysed
effects,
most
data
deriving
from
animal
studies.
review,
we
highlight
potential
influence
immature
subsequent
growth
differentiation
on
humans
animals.
Animals,
Journal Year:
2023,
Volume and Issue:
13(13), P. 2054 - 2054
Published: June 21, 2023
Artificial
insemination
is
an
important
assisted
reproductive
technology
that
has
been
applied
in
several
mammalian
species.
However,
successful
cryopreservation
of
semen
South
American
camelids
limited,
hindering
the
commercial
application
artificial
alpaca
In
this
scenario,
addition
antioxidants
to
extenders
provides
a
strategy
improve
freezability
sperm.
Bioactive
metabolites
from
natural
extracts
black
maca
have
shown
valuable
antioxidant
properties.
Thus,
objective
study
was
evaluate
effect
atomized
freezing
medium
epididymal
spermatozoa
alpacas.
Fifteen
pairs
epididymis
were
collected
local
slaughterhouse.
Each
sample
divided
into
six
groups:
(1)
fresh,
(2)
yolk
(YM),
(3)
10
mg/mL
maca,
(4)
20
(5)
30
and
(6)
resveratrol
(as
control).
Sperm
performed
through
slow
method.
Markers
associated
with
functionality,
such
as
motility,
viability,
plasma
membrane
integrity,
well
markers
oxidative
damage,
DNA
total
ROS
production,
mitochondrial
function,
analyzed.
The
results
show
supplementation
(20
mg/mL)
improved
sperm
function
evaluated
according
index
formazan
deposits.
Similarly,
production
decreased
at
mg/mL,
although
integrity
did
not
any
differences
among
groups.
These
suggest
cytoprotective
effects
during
freezing/thawing
Further
research
will
be
focused
on
assessing
by
using
biomolecular
(proAKAP4)
fertility.
Journal of Personalized Medicine,
Journal Year:
2023,
Volume and Issue:
13(12), P. 1654 - 1654
Published: Nov. 27, 2023
To
preserve
male
fertility
after
diagnosis
of
any
kind
cancer,
a
prompt
assessment
the
semen
quality
and
an
appropriate
cryopreservation
must
be
performed
before
radio-chemotherapy
starts.
The
present
work
aims
to
evaluate
parameters
at
different
cancer
patients
thawing.
Testicular
tumors
lymphomas
are
among
most
common
cancers
in
younger
patients,
while
chemotherapy
significantly
increases
patients'
survival,
it
can
epigenetically
alter
fluid,
resulting
temporary
or
permanent
infertility.
We
analyzed
data
from
database
Gamete
Cryopreservation
Center
(Annunziata
Hospital,
CS;
Italy)
period
2011-2020
cohort
254
aged
18-56
years.
evaluation
was
blind
manner
anonymously
recovered;
main
referring
were
assessed
accordance
with
WHO
guidelines
decision
limits
(6th
edition;
2021).
types
as
follows:
testis
(TC;
n
=
135;
53.1%),
hematological
(HC;
76;
29.9%),
other
(OC;
43;
17%).
Comparing
TC
vs.
HC
(P1)
OC
(P2),
had
worst
quality:
sperm
number/mL
(P1
0.0014;
P2
0.004),
total
motility
0.02;
0.07),
progressive
0.04;
0.05),
viability
0.01;
0.02),
percentage
atypical
morphology
0.05;
0.03).
After
thawing,
recovery
lowered,
accounting
for
46.82%
16.75%,
respectively,
whole
cohort;
similarly,
subgroups
ascribed
TC,
they
showed
lowest
recovery.
Strong
correlation
existed
between
pre-
post-cryopreservation
(p
<
0.001)
subgroup
0.05).
All
subgroups,
extents,
findings
below
reference
values,
suggesting
diverse
susceptibilities
cryodamage.
Cancer
associated
treatments
affect
quality,
meaning
should
considered
useful
personalized
prerogative
timely
manner.
