ERJ Open Research,
Journal Year:
2024,
Volume and Issue:
11(1), P. 00347 - 2024
Published: Oct. 10, 2024
Sputum
extracellular
DNA
(eDNA)
is
associated
with
disease
severity
in
asthma
and
COPD
therefore
emerging
as
a
potential
therapeutic
target.
The
aim
of
this
study
was
to
investigate
the
effect
10
days
recombinant
human
DNase
(rhDNase)
treatment
eDNA-high
on
sputum
eDNA
levels,
neutrophil-related
inflammation,
lung
function
symptoms.
Adults
(n=80)
or
(n=66)
were
screened
for
presence
high
(>20
µg·mL-1)
those
eligible
(n=18
asthma,
n=17
COPD)
randomised
two-period
crossover
controlled
trial
consisting
daily
nebulised
rhDNase
(2.5
mg/2.5
mL)
placebo
(5
mL
0.9%
saline)
days,
2-week
washout
period.
primary
outcome
eDNA,
secondary
outcomes
included
neutrophil
trap
(NET)-related
biomarkers,
inflammatory
cell
counts,
respiratory
At
screening,
significantly
higher
total
count,
colour
score
inflammation
(HNP1-3,
LL-37
interleukin-1β)
both
compared
low
groups.
In
participants
older
had
poorer
control
eDNA.
Administration
reduced
levels
(median
(Q1-Q3)
Pre:
48.4
(22.1-74.1);
Post:
17.0
(5.0-31.0)
µg·mL-1;
p=0.022)
39.3
(36.7-55.6);
25.4
(11.3-38.6)
p=0.044)
placebo.
Symptoms,
NET
biomarkers
remained
unchanged.
there
reduction
banded
blood
neutrophils
(3.2
(0-7.7)
0.0
(0.0-1.5);
p=0.044).
Targeted
effectively
COPD.
Journal Of Clinical Periodontology,
Journal Year:
2024,
Volume and Issue:
51(9), P. 1210 - 1221
Published: June 5, 2024
Abstract
Aim
To
explore
the
levels
of
neutrophil
extracellular
traps
(NETs)
in
patients
with
periodontitis
and
examine
their
effects
on
keratinization,
barrier
function
human
gingival
keratinocytes
(HGKs)
associated
mechanisms.
Materials
Methods
Saliva,
crevicular
fluid
(GCF),
clinical
periodontal
parameters
specimens
were
collected
from
10
healthy
control
subjects
stage
II–IV
to
measure
NET
levels.
Subsequently,
mRNA
protein
keratinization
indicators,
as
well
intracellular
calcium
epithelial
permeability,
analysed
HGKs
after
stimulation.
Results
The
study
showed
that
significantly
elevated
periodontitis,
across
multiple
including
saliva,
GCF
tissues.
Stimulation
NETs
resulted
a
decrease
expressions
involucrin,
cytokeratin
10,
zonula
occludens
1
E‐cadherin,
along
decreased
increased
permeability.
Furthermore,
inhibition
by
is
ERK‐KLF4‐dependent.
Conclusions
This
indicates
impair
suppress
through
ERK/KLF4
axis.
These
findings
provide
potential
targets
for
therapeutic
approaches
address
impaired
keratinization.
Frontiers in Pediatrics,
Journal Year:
2024,
Volume and Issue:
12
Published: June 7, 2024
In
contrast
to
significant
declines
in
deaths
due
lung
cancer
and
cardiac
disease
Westernised
countries,
the
mortality
‘chronic
obstructive
pulmonary
disease’
(COPD)
has
minimally
changed
recent
decades
while
‘the
incidence
of
bronchiectasis’
is
on
rise.
The
current
focus
producing
guidelines
for
these
two
airway
‘diseases’
hindered
progress
both
treatment
prevention.
elephant
room
that
neither
COPD
nor
bronchiectasis
a
but
rather
consequence
progressive
untreated
inflammation.
To
make
this
case,
it
important
review
evolution
our
understanding
how
pathological
appearance
(bronchiectasis)
an
arbitrary
physiological
marker
impaired
airways
came
be
labelled
as
‘diseases’.
Valuable
insights
into
natural
history
can
obtained
from
pre-antibiotic
era.
dramatic
impacts
antibiotics
prevalence
disease,
especially
childhood
early
adult
life,
have
largely
been
forgotten
will
revisited
misinterpretation
trials
undertaken
those
with
chronic
(bacterial)
bronchitis.
past
decades,
paediatricians
observed
increase
what
termed
‘persistent
bacterial
bronchitis’
(PBB).
This
condition
shares
all
same
characteristics
bronchitis’,
which
prevalent
young
children
during
Additionally,
radiological
once
again
becoming
more
common
and,
recently,
adults.
Adult
physicians
remain
sceptical
about
existence
PBB;
however,
one
study
aimed
at
assessing
efficacy
adults
persistent
symptoms,
researchers
discovered
majority
patients
exhibiting
symptoms
PBB
were
already
long-term
macrolides.
there
growing
recognition
importance
respiratory
microbiome
ability
bacteria
persist
potentially
hostile
environments
through
strategies
such
biofilms,
intracellular
communities,
persister
bacteria.
challenging
field
likely
require
new
approaches
diagnosis
treatment;
needs
embraced
if
real
made.
PubMed,
Journal Year:
2024,
Volume and Issue:
26(7), P. 723 - 729
Published: July 15, 2024
To
explore
the
diagnostic
efficacy
of
serum
14-3-3β
protein
combined
with
fractional
exhaled
nitric
oxide
(FeNO)
and
conventional
ventilatory
lung
function
parameters
in
diagnosing
bronchial
asthma
(referred
to
as
"asthma")
children.
ERJ Open Research,
Journal Year:
2024,
Volume and Issue:
11(1), P. 00347 - 2024
Published: Oct. 10, 2024
Sputum
extracellular
DNA
(eDNA)
is
associated
with
disease
severity
in
asthma
and
COPD
therefore
emerging
as
a
potential
therapeutic
target.
The
aim
of
this
study
was
to
investigate
the
effect
10
days
recombinant
human
DNase
(rhDNase)
treatment
eDNA-high
on
sputum
eDNA
levels,
neutrophil-related
inflammation,
lung
function
symptoms.
Adults
(n=80)
or
(n=66)
were
screened
for
presence
high
(>20
µg·mL-1)
those
eligible
(n=18
asthma,
n=17
COPD)
randomised
two-period
crossover
controlled
trial
consisting
daily
nebulised
rhDNase
(2.5
mg/2.5
mL)
placebo
(5
mL
0.9%
saline)
days,
2-week
washout
period.
primary
outcome
eDNA,
secondary
outcomes
included
neutrophil
trap
(NET)-related
biomarkers,
inflammatory
cell
counts,
respiratory
At
screening,
significantly
higher
total
count,
colour
score
inflammation
(HNP1-3,
LL-37
interleukin-1β)
both
compared
low
groups.
In
participants
older
had
poorer
control
eDNA.
Administration
reduced
levels
(median
(Q1-Q3)
Pre:
48.4
(22.1-74.1);
Post:
17.0
(5.0-31.0)
µg·mL-1;
p=0.022)
39.3
(36.7-55.6);
25.4
(11.3-38.6)
p=0.044)
placebo.
Symptoms,
NET
biomarkers
remained
unchanged.
there
reduction
banded
blood
neutrophils
(3.2
(0-7.7)
0.0
(0.0-1.5);
p=0.044).
Targeted
effectively
COPD.
