Digital holo-tomographic 3D maps of COVID-19 microclots in blood to assess disease severity DOI Creative Commons
Talia Bergaglio, Olena Synhaivska, Peter Niraj Nirmalraj

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 12, 2023

Abstract The coronavirus disease 2019 (COVID-19) has impacted health globally. Cumulative evidence points to long-term effects of COVID-19 such as cardiovascular and cognitive disorders diagnosed in patients even after the recovery period. In particular, micrometer-sized blood clots hyperactivated platelets have been identified potential indicators long COVID. Here we resolve individual microclot structures platelet-rich plasma donors with different subphenotypes a label-free manner, using 3D digital holo-tomographic microscopy (DHTM). Based on refractive index (RI) tomograms, size, dry mass, prevalence composites were quantified then parametrically differentiated from fibrin-rich microclots platelet aggregates donors. Importantly, fewer detected healthy controls when compared Our work highlights utility integrating DHTM clinical settings that may allow detection individuals at risk developing microvascular thrombotic for monitoring efficiency prescribed treatments by screening samples.

Language: Английский

SARS-CoV-2 S1 protein induces IgG-mediated platelet activation and is prevented by 1.8-cineole DOI

Julie Petry,

Maria Shoykhet,

Tobias Weiser

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 187, P. 118100 - 118100

Published: April 29, 2025

Language: Английский

Citations

0
3D Holo-tomographic Mapping of COVID-19 Microclots in Blood to Assess Disease Severity DOI Creative Commons
Talia Bergaglio, Olena Synhaivska, Peter Niraj Nirmalraj

et al.

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2(3), P. 194 - 204

Published: Jan. 10, 2024

The coronavirus disease 2019 (COVID-19) has impacted health globally. Cumulative evidence points to long-term effects of COVID-19 such as cardiovascular and cognitive disorders, diagnosed in patients even after the recovery period. In particular, micrometer-sized blood clots hyperactivated platelets have been identified potential indicators long COVID. Here, we resolve microclot structures plasma with different subphenotypes a label-free manner, using 3D digital holo-tomographic microscopy (DHTM). Based on refractive index (RI) tomograms, size, dry mass, prevalence composites were quantified then parametrically differentiated from fibrin-rich microclots platelet aggregates patients. Importantly, fewer detected healthy controls compared Our imaging analysis workflow is built around commercially available DHT microscope capable operation clinical settings 2 h time period sample preparation data acquisition results.

Language: Английский

Citations

3
Complex Pattern of Platelet Activation/Reactivity After SARS-CoV-2 Infection DOI Open Access
Bogusława Luzak, Jacek Golański, M. Różalski

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 49 - 49

Published: Dec. 24, 2024

COVID-19 and post-COVID (long COVID) are associated with thromboembolic complications; however, it is still not clear whether platelets play a leading role in this phenomenon. The platelet hyperreactivity could result from the direct interaction between viral elements or response to inflammatory prothrombotic factors released blood vessel cells following infection. existing literature does provide clear-cut answers, as results determining status vary according methodology. Elevated levels of soluble markers activation (P selectin, PF4), increased aggregates, platelet-derived microparticles suggest circulating bloodstream patients. Similarly, isolated patients demonstrate reactivity collagen, thrombin, ADP. By contrast, an analysis whole indicates reduced fibrinogen receptor. some vitro studies report potential targets for SARS-CoV-2 platelets, whereas others do indicate any effect virus on platelets. aim work review evaluate reliability methodology testing function after contact SARS-CoV-2. Despite diversity methods yielding varying influence plasma components cells, can be concluded that important development thrombotic complications exposure

Language: Английский

Citations

1
Digital holo-tomographic 3D maps of COVID-19 microclots in blood to assess disease severity DOI Creative Commons
Talia Bergaglio, Olena Synhaivska, Peter Niraj Nirmalraj

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 12, 2023

Abstract The coronavirus disease 2019 (COVID-19) has impacted health globally. Cumulative evidence points to long-term effects of COVID-19 such as cardiovascular and cognitive disorders diagnosed in patients even after the recovery period. In particular, micrometer-sized blood clots hyperactivated platelets have been identified potential indicators long COVID. Here we resolve individual microclot structures platelet-rich plasma donors with different subphenotypes a label-free manner, using 3D digital holo-tomographic microscopy (DHTM). Based on refractive index (RI) tomograms, size, dry mass, prevalence composites were quantified then parametrically differentiated from fibrin-rich microclots platelet aggregates donors. Importantly, fewer detected healthy controls when compared Our work highlights utility integrating DHTM clinical settings that may allow detection individuals at risk developing microvascular thrombotic for monitoring efficiency prescribed treatments by screening samples.

Language: Английский

Citations

2