Variations in the expression of odorant binding and chemosensory proteins in the developmental stages of whitefly Bemisia tabaci Asia II-1

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 1, 2024

Abstract The cotton whitefly, Bemisia tabaci , is considered as a species complex with 46 cryptic species, Asia II-1 being predominant in Asia. This study addresses significant knowledge gap the characterization of odorant-binding proteins (OBPs) and chemosensory (CSPs) II-1. We explored expression patterns OBPs CSPs throughout their developmental stages compared motif these proteins. Significant differences were observed for 14 B. II-1, OBP8 CSP4 showing higher across stages. Phylogenetic analysis reveals that form distinct clades, appearing to be an ancestral gene, giving rise evolution other . genomic distribution highlights gene clustering on chromosomes, suggesting functional conservation evolutionary events following birth-and-death model. Molecular docking studies indicate strong binding affinities various odour compounds like β-caryophyllene, α-pinene, β-pinene limonene, reinforcing roles host recognition reproductive functions. elaborates our understanding putative different hitherto unexplored. dynamics interactions offer scope developing innovative methods controlling this global invasive pest.

Language: Английский

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The binding affinity of two general odorant binding proteins in Spodoptera frugiperda to general volatiles and insecticides

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 252, P. 126338 - 126338

Published: Aug. 15, 2023

Language: Английский

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Binding Properties of the General Odorant-Binding Protein GOBP2 to Herbicides and Insecticides in Spodoptera litura

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

We previously reported that the general odorant-binding protein GOBP2 enhances chlorpyrifos tolerance in Spodoptera litura by perceiving herbicides. However, direct interaction between and pesticides remains unknown. Herein, we verified effect of binding to on herbicide-induced insecticide S. litura. Fluorescence competitive assays indicated exhibits high affinities herbicide trifluralin insecticides indoxacarb, chlorpyrifos, fipronil, with Ki values ranging from 1.95 13.01 μM. Moreover, Ala136 Thr30 were determined as key sites through molecular docking site-directed mutagenesis. Finally, knockdown significantly increased larval susceptibility three types insecticides. Our findings provide a valuable reference for further exploration mechanisms underlying litura, laying foundation innovative pest management strategies.

Language: Английский

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Genome-Wide Identification and Expression Profiling of Odorant-Binding Protein Genes in the Bean Flower Thrips Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae)

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 212 - 212

Published: Feb. 14, 2025

Megalurothrips usitatus is an economically important vegetable pest. Because of the growing demand for reducing pesticide use on vegetables, new environmentally friendly strategies controlling M. are urgently needed. Insect odorant-binding proteins prospective targets screening odorant attractants pest control. However, very little known about OBP genes in usitatus. Here, we identified 14 OBPs genome using HMMER and BLAST. The chromosomal location showed that these were widely distributed across eight chromosomes. analysis gene protein structure characteristics revealed substantial diversity within family. spatiotemporal expression profiles ten out MusiOBPs displayed male biased expression, which highly expressed antennae, suggesting they may play a crucial role recognition host plant volatiles thrips aggregation pheromones. Notably, only MusiOBP8 was significantly higher female adults, indicating potential involvement reproduction. Moreover, MusiOBP7 MusiOBP13 pupae, their possible immune responses. These results provide foundation further exploration functions

Language: Английский

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Chemosensory protein 16 has an immune function and participates in host-pathogen interaction in Galleria mellonella infected with Pseudomonas entomophila

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 28, 2025

Chemosensory protein 16 was identified in the hemolymph of Galleria mellonella as a with an amount increasing after oral infection 10^3 CFU Pseudomonas entomophila, and decreasing higher dose (10^5 CFU) bacteria. The expression CSP16 gene occurred fat body gut correlated changes level hemolymph. inhibited P. entomophila growth concentration range from 0.15 to 6 nM. Additionally, showed bactericidal activity against Bacillus thuringiensis, Escherichia coli 2–18 μM, but only presence protease inhibitors, otherwise it degraded by extracellular proteases secreted entomophila. We demonstrated that related its ability perforate bacterial cellular membranes dose-dependent manner. antimicrobial properties this were also confirmed use Atomic Force Microscopy, which significant topology different cell surfaces. Finally, when injected vivo into G. larvae one hour more survivors observed at particular time-points. Taking account immune putative bind bacteria-derived compounds, possible function host-pathogen interaction is discussed.

Language: Английский

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Expression and sex pheromone-binding characteristics of pheromone-binding protein 3 in Tuta absoluta (Lepidoptera: Gelechiidae)

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106404 - 106404

Published: April 1, 2025

Language: Английский

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Serotonin/GABA receptors modulate odor input to olfactory receptor neuron in locusts

Frontiers in Cellular Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: April 28, 2023

Serotonin (5-hydroxytryptamine; 5-HT) and GABA (γ-aminobutyric acid) are involved in the regulation of behaviors central nervous system. However, it remains unclear whether they modulate olfaction peripheral system, how olfaction.One 5-HT receptor sequence (Lmig5-HT2) one (LmigGABAb) were identified locust antennae by transcriptome analysis polymerase chain reaction experiments. In situ hybridization localized Lmig5-HT2 to accessory cells, while LmigGABAb was olfactory neurons (ORNs) chemosensilla. Single-unit electrophysiological recordings combined with RNA interference (RNAi) experiments indicated ORNs locusts knockdown (ds-Lmig5-HT2) (ds-LmigGABAb) some odors had significantly higher responses than wild-type control dose-dependent responses. Moreover, gaps between RNAi ones those ds-GFP enlarged an increase concentrations odors.Taken together, our findings suggest that 5-HT, GABA, their receptors exist insect system may function as negative feedback contribute a fine-tuning mechanism for

Language: Английский

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Binding Properties of Odorant-Binding Protein 7 to Host Volatiles in Larvae of Spodoptera frugiperda

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(51), P. 20671 - 20679

Published: Dec. 16, 2023

The chemosensory system is crucial during the growth and development of moths. Spodoptera frugiperda (Lepidoptera: Noctuidae) one most destructive insect pests. However, there little functional research on odorant-binding proteins (OBPs) in larval stage S. frugiperda. Here, we obtained SfruOBP7 from transcriptomics conducted sequence analysis. We used quantitative real-time PCR to explore expression profiles SfruOBP7. function identification showed that has a binding ability 18 plant volatiles. Further molecular docking site-directed mutant assay revealed Lys45 Phe110 were key sites for interacting with linalool. In behavior assays, linalool could attract larvae, dsOBP7-treated larvae lost their attraction Our results help reveal essential mechanism olfactory perception design an attractant based host

Language: Английский

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Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 10

Published: Jan. 25, 2024

In insects, antennal ionotropic receptors (IRs) and odorant (ORs) are among the main sensors of olfactory cues. To functionally characterize subunits from these receptors, use ab3A neurons transgenic Drosophila melanogaster represented one most powerful tools, allowing identification ligands (deorphanization) decrypting their pharmacological properties. However, further investigation is needed to shed light on possible metabotropic functionalities behind insect test potentials up-to-now-used empty neuronal systems express belonging variegate receptor classes. this project, we adopted updated system various ranging human ORs working as G-protein coupled IRs ORs. Testing expressing into by single sensillum recording did not result in an OR response ligands, but it rather re-established spiking neurons. When D. expressed ORs, both heterologous cis-expressed were non-functional, suzukii OR19A1 subunit responded a wide asset distinguishing phasic or tonic compound-dependent effects. Despite activation some resulted study deorphanized key demonstrating its binding alcohols, ketones, terpenes, esters.

Language: Английский

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Identification of attractants for adult Spodoptera litura based on the interaction between odorant-binding protein 34 and host volatiles

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 203, P. 106005 - 106005

Published: July 1, 2024

Language: Английский

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