Label-free electrochemical immunosensor employing new redox probes/porous organic polymers/graphene oxide nanocomposite towards multiplex detection of three SARS-COV2-induced storming proteins for severe COVID-19 diagnosis

Materials Today Chemistry, Journal Year: 2024, Volume and Issue: 35, P. 101906 - 101906

Published: Jan. 1, 2024

Language: Английский

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Serum Insulin-like Growth Factor-Binding Protein-2 as a Prognostic Factor for COVID-19 Severity

Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 125 - 125

Published: Jan. 8, 2024

Insulin-like growth factor-binding protein (IGFBP)-2 is a regulator of anabolic pathways, which become inactivated in severe illness. Here, we measured the serum IGFBP-2 levels COVID-19 patients with moderate and disease as well healthy controls to identify associations severity. Patients had higher than those controls, who similar levels. Non-survivors tended have elevated compared survivors. Increased were observed requiring dialysis vasopressor therapy. Serum was positively correlated procalcitonin both patient groups. Bacterial co-infection did not influence liver cirrhosis obesity, showing increased decreased levels, respectively, excluded from study. The present analysis showed that are associated severity patients. similarity between suggests critical illness rather SARS-CoV-2 infection itself.

Language: Английский

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Cytokine Storm in COVID-19: Exploring IL-6 Signaling and Cytokine-Microbiome Interactions as Emerging Therapeutic Approaches

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11411 - 11411

Published: Oct. 24, 2024

IL-6 remains a key molecule of the cytokine storms characterizing COVID-19, exerting both proinflammatory and anti-inflammatory effects. Emerging research underscores significance trans-signaling over classical signaling pathways, which has shifted focus therapeutic strategies. Additionally, synergistic action TNF-α IFN-γ been found to induce inflammatory cell death through PANoptosis, further amplifying severity storms. Long COVID-19 patients, as well those with triggered by other conditions, exhibit distinct laboratory profiles, indicating need for targeted approaches diagnosis management. Growing evidence also highlights gut microbiota's crucial role in modulating immune response during affecting production, adding complexity disease's immunological landscape. Targeted intervention strategies should on specific cutoffs, though accurate quantification clinical challenge. Current treatment are increasingly focused inhibiting trans-signaling, offers promise more precise manage hyperinflammatory responses COVID-19. In light recent discoveries, this review summarizes findings storms, particularly their conditions. It explores emerging targeting cytokines like IL-6, TNF-α, IFN-γ, while addressing open questions, such better biomarkers detect ongoing challenges developing treatments that mitigate hyperinflammation without compromising function, emphasizing importance continued field.

Language: Английский

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Tumor necrosis factor superfamily signaling: life and death in cancer

Cancer and Metastasis Reviews, Journal Year: 2024, Volume and Issue: 43(4), P. 1137 - 1163

Published: Oct. 4, 2024

Language: Английский

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Latex of medicinal plants: a reservoir of antimicrobial peptides, proteins, and enzymes for drug discovery

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 13, 2025

The exploration of latex from medicinal plants has unveiled a promising source antimicrobial peptides (AMPs), proteins and enzymes with significant therapeutic potentials. Despite their value, these sources remain underutilized. Latex, complex, viscous fluid secreted by specific plants, in the Euphorbiaceae, Apocynaceae, Moraceae, Papaveraceae, Asclepiadaceae families, serves multiple functions, including wound healing, protection against herbivores, most importantly, as defense microbial pathogens This review provides an overview latex-producing emphasizing role discovery molecules. Notable among is Hevea brasiliensis, hevein hevein-like peptides, which have demonstrated antifungal antibacterial activities. Other key AMPs are cysteine-rich lectins that exhibit potent properties. Additionally, peptidases proteases obtained garnered attention for potential both activity enhancement peptide stability efficacy. These molecules act through unique mechanisms, disrupting cell membranes, making them valuable candidates fight multidrug-resistant pathogens. They underscore contribution to modern research. While discoveries hold great promises, some challenges still exist. Interestingly, biotechnology advancements synthesis genetic engineering improved latex-derived biomolecules enabling large-scale production modification, enhancing stability, potency, effectiveness use. Also, integration genomic proteomic tools will further unlock full potentials, paving way novel agents capable addressing antibiotic resistance.

Language: Английский

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2-Ethylhexyl diphenyl phosphate induces lung oxidative stress and pyroptosis in chicks

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 962, P. 178453 - 178453

Published: Jan. 1, 2025

Language: Английский

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The interplay of tuberculosis and COVID-19: Insights into global health challenges

Journal of Biosciences, Journal Year: 2025, Volume and Issue: 50(1)

Published: Feb. 22, 2025

Language: Английский

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Cytokine storm polymorphisms in nonvaccinated COVID-19 patients

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Abstract Cytokines and chemokines are essential for establishing an appropriate immune response to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Variations in the genes encoding cytokines strongly influence pathogenic challenges disease outcomes. This study was carried out determine associations of polymorphisms TNF -α , IL-6 IL-8, IL-10 CCL5 with COVID-19 severity. A total 627 unvaccinated patients were classified according WHO We evaluated levels IFN-α, IFN-γ, TNF-α, IL-1R, IL-6, IL-7, IL-10, CCL2, CCL3, CXCL8, CXCL10 GCSF serum compared among severity groups stratified by polymorphism alleles. revealed a significant increase IL-2, CCL-2 dead group. However, higher moderate group than mild Logistic regression analysis that five associated increased risk COVID-19: (rs1800610) allele (OR=1.50; 95% CI: 1.01–2.24); (rs1800796) C (OR=1.64; 1.05–2.57); (rs1800871) T (OR=1.94; 1.24–3.04) (rs1800872) (OR=1.87; 1.21–2.89); (rs3817656) G (OR= 1.64; 1.02–2.65)). The (rs1800871 rs1800872) (rs1800796 rs18049563) gene also Increases carriers rs1049953. In contrast, not any SNPs.

Language: Английский

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Alveolar epithelial type 2 cell specific loss of IGFBP2 activates inflammation in COVID-19

Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 22, 2025

Abstract The coronavirus disease 2019 (COVID-19) global pandemic is caused by severe acute respiratory syndrome 2 (SARS-CoV-2). However, our understanding of SARS-CoV-2-induced inflammation in alveolar epithelial cells remains very limited. contributions intracellular insulin-like growth factor binding protein-2 (IGFBP2) to SARS-CoV-2 pathogenesis are also unclear. In this study, we have uncovered a critical role for IGFBP2, specifically type (AEC2), the immunopathogenesis COVID-19. Using bulk RNA sequencing, show that IGFBP2 mRNA expression significantly downregulated primary AEC2 isolated from fibrotic lung regions patients with COVID-19-acute distress (ARDS) compared those idiopathic pulmonary fibrosis (IPF) alone or IPF history multicolor immunohistochemistry, demonstrated and its selective ligands IGF1 IGF2 were reduced COVID-ARDS, alone, COVID than age-matched donor controls. Further, lentiviral Igfbp2 proinflammatory cytokines—Tnf-α, Il1β, Il6, Stat3, Stat6 chemokine receptors—Ccr2 Ccr5—in mouse challenged spike protein injury (S2; 500 ng/mL). Finally, higher levels cytokines—TNF-α; IL-6 receptor—CCR5 COVID-ARDS patients. Altogether, these data suggest anti-inflammatory properties localized delivery may serve as potential therapeutic strategy Graphical

Language: Английский

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Beyond interferon gamma - decreased cellular response to COVID-19 vaccination booster in patients with autoimmune inflammatory rheumatic diseases

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 28, 2025

The global COVID-19 pandemic has led to significant advancements in vaccine research, particularly regarding patients with autoimmune inflammatory rheumatic diseases (AIIRD). However, most studies have assessed the post-vaccination cellular response only by measuring production of interferon-gamma. This study aimed explore immune AIIRD, a focus on effects immunomodulatory drugs different proteins involved and cytotoxicity. We analyzed blood samples from 54 - 16 healthy controls (HC) 38 AIIRD at three time points: before (T0), 4 weeks after (T1), more than 6 months (T2) booster vaccination. Gene expression concentration levels 13 immunity were assessed. Our showed significantly reduced TNF T0, IL-2 T0 T2, perforin T2 compared HC. In genes cytotoxicity, including NRF2, TRAIL, cathepsin B, H was impaired. Both protein concentrations gene altered those treated glucocorticoids, methotrexate, biologic/targeted synthetic disease-modifying antirheumatic (b/tsDMARDs). Among b/tsDMARDs IL-17 inhibitors did not affect response. These findings suggest that vaccination elicits weakened immunosuppressive therapies, potentially compromising efficacy. Further are required determine clinical impact these long-term effectiveness this population.

Language: Английский

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