Molecular Mechanism of SOX18 in Lipopolysaccharide-Induced Injury of Human Umbilical Vein Endothelial Cells DOI
Jian Luo, Honglong Fang,

Danqiong Wang

et al.

Critical Reviews in Immunology, Journal Year: 2023, Volume and Issue: 44(3), P. 1 - 12

Published: Nov. 27, 2023

Endothelial dysfunction is associated with the progression of sepsis. This study sought to probe molecular route sex-determining region on Y chromosome-box transcription factor 18 (SOX18) in sepsis-associated endothelial injury. Human umbilical vein cells (HUVECs) were treated lipopolysaccharide (LPS) establish sepsis cell model. Cell viability, lactate dehydrogenase (LDH) release, oxidative stress (reactive oxygen species/malondialdehyde/superoxide dismutase), and inflammation (interleukin-1β/tumor necrosis factor-α/interleukin-6) evaluated by counting kit-8 assay relevant kits. The expression levels SOX18, microRNA (miR)-204-5p, cadherin-2 (CDH2) determined real-time quantitative polymerase chain reaction Western blot assay. interaction miR-204-5p, CDH2 was analyzed chromatin immunoprecipitation dual-luciferase LPS induced HUVECs injury downregulation SOX18. SOX18 overexpression increased while decreased LDH activity, stress, inflammation. bound miR-204-5p promoter promote expression, further repressed expression. knockdown abrogated protective role Overall, alleviated LPS-induced promoting repressing CDH2, suggesting it as a potential target for treatment.

Language: Английский

Long Non-Coding RNAs in Malignant Human Brain Tumors: Driving Forces Behind Progression and Therapy DOI Open Access

Dakun Pei,

Dandan Zhang, Yan Guo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 694 - 694

Published: Jan. 15, 2025

Long non-coding RNAs (lncRNAs) play a pivotal role in regulating gene expression and are critically involved the progression of malignant brain tumors, including glioblastoma, medulloblastoma, meningioma. These lncRNAs interact with microRNAs (miRNAs), proteins, DNA, influencing key processes such as cell proliferation, migration, invasion. This review highlights multifaceted impact lncRNA dysregulation on tumor underscores their potential therapeutic targets to enhance efficacy chemotherapy, radiotherapy, immunotherapy. The insights provided offer new directions for advancing basic research clinical applications tumors.

Language: Английский

Citations

0
Correlation Between Long non-coding RNA SOX2OT rs9839776 C>T Polymorphism and Unexplained Recurrent Miscarriages DOI Creative Commons

Nermeen Abd Ellatif,

Sara H. A. Agwa, Neveen A. Ashaat

et al.

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology/Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, Journal Year: 2024, Volume and Issue: 16(1), P. 277 - 295

Published: March 22, 2024

Recurrent miscarriage (RM) is a severe pregnancy complication that results in the loss of two or more spontaneous pregnancies. RM has several complex reasons. Advances genetics, immunology, and cell biology have strongly connected non-coding RNAs (ncRNAs) to recurrent miscarriages. NcRNAs regulate placental trophoblast processes, which affect development, migration, invagination; they also role breast ovarian cancer. As result, their abnormal expression may contribute progression RM. Several investigations found connection between genetic polymorphisms migration. Variations long RNA (lncRNA) SOX2OT gene been associated with variety cancer-related illnesses, particularly colorectal cancer, cancer digestive tract. According recent study, rs9839776 CT raises risk In this review, we explain existing evidence supporting relationship LncRNA

Language: Английский

Citations

0
Molecular Mechanism of SOX18 in Lipopolysaccharide-Induced Injury of Human Umbilical Vein Endothelial Cells DOI
Jian Luo, Honglong Fang,

Danqiong Wang

et al.

Critical Reviews in Immunology, Journal Year: 2023, Volume and Issue: 44(3), P. 1 - 12

Published: Nov. 27, 2023

Endothelial dysfunction is associated with the progression of sepsis. This study sought to probe molecular route sex-determining region on Y chromosome-box transcription factor 18 (SOX18) in sepsis-associated endothelial injury. Human umbilical vein cells (HUVECs) were treated lipopolysaccharide (LPS) establish sepsis cell model. Cell viability, lactate dehydrogenase (LDH) release, oxidative stress (reactive oxygen species/malondialdehyde/superoxide dismutase), and inflammation (interleukin-1β/tumor necrosis factor-α/interleukin-6) evaluated by counting kit-8 assay relevant kits. The expression levels SOX18, microRNA (miR)-204-5p, cadherin-2 (CDH2) determined real-time quantitative polymerase chain reaction Western blot assay. interaction miR-204-5p, CDH2 was analyzed chromatin immunoprecipitation dual-luciferase LPS induced HUVECs injury downregulation SOX18. SOX18 overexpression increased while decreased LDH activity, stress, inflammation. bound miR-204-5p promoter promote expression, further repressed expression. knockdown abrogated protective role Overall, alleviated LPS-induced promoting repressing CDH2, suggesting it as a potential target for treatment.

Language: Английский

Citations

1