Frontiers in Genetics,
Journal Year:
2025,
Volume and Issue:
16
Published: May 15, 2025
Introduction
Atractylodes
lancea
(Thunb.)
DC.,
a
widely
utilized
herb
in
traditional
Chinese
medicine,
contains
sesquiterpenoids
and
polyacetylenes
as
its
primary
bioactive
components.
The
WRKY
gene
family
plays
critical
role
regulating
various
biological
processes
plants.
However,
the
molecular
mechanism
underlying
AlWRKY
regulation
of
terpenoid
synthesis
unclear.
Methods
members
were
identified
through
bioinformatics
approaches.
Gene
structures,
motifs,
phylogenetic
relationships
analyzed.
Subsequently,
their
expression
profiles
across
different
geographical
origins
investigated
using
transcriptome
data.
Furthermore,
preliminary
validation
was
performed
via
methyl
jasmonate
treatment
docking,
with
focus
on
AlWRKY20
AlWRKY37
genes.
Results
In
this
study,
65
genes
conserved
domains
A.
classified
into
three
groups:
Group
I
(17
members),
II
(33
III
(15
members).
Tissue-specific
profiling
revealed
five
rhizome-enriched
(
AlWRKY13
,
AlWRKY21
AlWRKY49
)
highly
expressed
Hubei
accessions
compared
to
Jiangsu
accessions,
co-expression
analysis
demonstrated
strong
correlation
16
AlTPS
Quantitative
PCR
(qPCR)
confirmed
specific
upregulation
rhizomes,
consistent
accumulation
patterns
sesquiterpenes
(hinesol,
γ-eudesmol,
elemol).
Methyl
(MeJA)
induction
experiments
(12
h)
coordinated
AlTPS70
AlTPS71
concomitant
significantly
increased
cis-β-farnesene
α-curcumene
content.
Molecular
docking
binding
affinities
AlTPS70/AlTPS71
promoter
promoter.
Subcellular
localization
that
both
are
localized
nucleus.
These
results
suggest
likely
function
regulators
sesquiterpene
biosynthesis,
positively
production
modulation.
Discussion
This
study
lays
groundwork
for
further
exploration
mechanisms
functional
transcription
factors
.
Critical Reviews in Biotechnology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 15
Published: April 23, 2025
Climate
change
induces
various
environmental
stressors
that
restrict
plant
processes,
thereby
limiting
overall
crop
productivity.
Plant
secondary
metabolites
(SMs)
enable
plants
to
quickly
detect
a
broad
array
of
and
respond
in
accordance
rapidly
changing
scenarios.
Notably,
SMs
regulate
defense
signaling
cascades
provide
defensive
functions
safeguard
against
biotic
abiotic
stressors.
In
this
review,
we
an
overview
insights
into
recent
advances
types
biosynthetic
pathways
SMs.
We
emphasize
the
mechanisms
different
elicitors-induced
synthesis
accumulation
responses.
addition,
SMs-mediated
regulation
processes
act
through
phytohormones
is
discussed.
Finally,
show
transcriptional
factors
regulating
biosynthesis
associated
regulatory
networks
could
be
used
for
creating
resilient
plants.
Overall,
comprehensive
review
gives
insight
regarding
crucial
roles
enhanced
resistance
provides
new
ideas
development
stress-resistant
varieties
under
current
climate
Frontiers in Genetics,
Journal Year:
2025,
Volume and Issue:
16
Published: May 15, 2025
Introduction
Atractylodes
lancea
(Thunb.)
DC.,
a
widely
utilized
herb
in
traditional
Chinese
medicine,
contains
sesquiterpenoids
and
polyacetylenes
as
its
primary
bioactive
components.
The
WRKY
gene
family
plays
critical
role
regulating
various
biological
processes
plants.
However,
the
molecular
mechanism
underlying
AlWRKY
regulation
of
terpenoid
synthesis
unclear.
Methods
members
were
identified
through
bioinformatics
approaches.
Gene
structures,
motifs,
phylogenetic
relationships
analyzed.
Subsequently,
their
expression
profiles
across
different
geographical
origins
investigated
using
transcriptome
data.
Furthermore,
preliminary
validation
was
performed
via
methyl
jasmonate
treatment
docking,
with
focus
on
AlWRKY20
AlWRKY37
genes.
Results
In
this
study,
65
genes
conserved
domains
A.
classified
into
three
groups:
Group
I
(17
members),
II
(33
III
(15
members).
Tissue-specific
profiling
revealed
five
rhizome-enriched
(
AlWRKY13
,
AlWRKY21
AlWRKY49
)
highly
expressed
Hubei
accessions
compared
to
Jiangsu
accessions,
co-expression
analysis
demonstrated
strong
correlation
16
AlTPS
Quantitative
PCR
(qPCR)
confirmed
specific
upregulation
rhizomes,
consistent
accumulation
patterns
sesquiterpenes
(hinesol,
γ-eudesmol,
elemol).
Methyl
(MeJA)
induction
experiments
(12
h)
coordinated
AlTPS70
AlTPS71
concomitant
significantly
increased
cis-β-farnesene
α-curcumene
content.
Molecular
docking
binding
affinities
AlTPS70/AlTPS71
promoter
promoter.
Subcellular
localization
that
both
are
localized
nucleus.
These
results
suggest
likely
function
regulators
sesquiterpene
biosynthesis,
positively
production
modulation.
Discussion
This
study
lays
groundwork
for
further
exploration
mechanisms
functional
transcription
factors
.
