bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 3, 2024
Abstract
Small,
gaseous
molecules,
known
as
gasotransmitters
(NO,
CO,
H
2
S),
are
produced
endogenously
in
mammalian
cells
and
serve
important
biological
roles.
Hydrogen
cyanide,
traditionally
considered
a
cytotoxic
molecule
mammals,
serves
an
endogenous
mediator
several
plants
bacterial
species.
Here
we
show
that
low
concentrations
of
cyanide
generated
mouse
liver
human
hepatocytes.
Cyanide
production
is
stimulated
by
glycine,
occurs
at
the
pH
lysosomes
requires
peroxidase
activity.
Cyanide,
turn,
detectable
cellular
compartments.
also
basally
blood
mice;
its
levels
increase
after
treatment
animals
with
glycine.
Rhodanese
activity
regulates
levels.
when
optimal
level,
exerts
stimulatory
effects
on
mitochondrial
bioenergetics,
cell
metabolism
proliferation.
Dysregulation
either
below
or
above
levels,
impairs
bioenergetics.
The
regulatory
part
mediated
posttranslational
modification
cysteine
residues
via
protein
cyanylation;
cyanylated
can
be
detected
basally,
Controlled
low-dose
supplementation
exhibits
cytoprotective
effects,
demonstrated
hypoxia
reoxygenation
models
vitro
vivo
.
However,
pathologically
elevated
production,
nonketotic
hyperglycinemia
–
autosomal
recessive
disease
glycine
deleterious
to
cells.
