Construction of ceRNA networks in endocrine therapy resistance model systems DOI Creative Commons

S. Pramanik,

Partha Pratim Das, Monalisa Mukherjee

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 17, 2024

ABSTRACT Background Endocrine therapy resistance (ETR) in breast cancer is achieved via multiple pathways including a decrease ER, dysregulation of cell cycle genes, and/or mutations ER/co-activators/co-repressors. We have reported earlier that high expression Jumonji domaining containing protein 6 (JMJD6) induced ETR by depleting ER expression. In this study, 3 cellular models representing distinct pathways; Tamoxifen resistant (TAMR), Long-term Estrogen deprived (LTEDI), JMJD6 overexpressing (JOE) cells, and parental MCF7 were subjected to RNA-sequencing, CNC, ceRNA network analysis. hypothesised post-comparison RNA regulations are common all lines, will reveal actionable markers targets. These be shared patients with ET-resistant disease, independent the initiating event. Results 170 differentially expressed genes found, these, 73 maintained same directionality (ETR cassette genes). segregated TCGA ER+ tumors into two groups, one intermixing ER-tumors. Pathway-based curation identified 21 (7 up- 14 down-regulated) participated hallmark pathways. Genes upregulated cells less at diagnosis when compared normal samples but their higher indicated adverse survival outcomes. Next, these used for CNC construction triad FLT4:MIR503HG:miR-497/195/424 was discovered. The levels miRNAs predicted analysis quantitative RT-PCR validate down regulation miR-497/195/424 upregulation targets, FLT4 MIR503HG cells. Conclusions show total RNA-seq data can successfully predict achieve drug resistance. Re-expression such as tumor diagnosis, may indicative onset. Finally, arise due suppression miR-424/497/195 leading MIR503HG. posit suitable target could developed detection strategy cancer.

Language: Английский

Unveiling the connection: Long-chain non-coding RNAs and critical signaling pathways in breast cancer DOI
Riya Thapa, Obaid Afzal, Gaurav Gupta

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 249, P. 154736 - 154736

Published: Aug. 3, 2023

Language: Английский

Citations

93
lncRNA-microRNA axis in cancer drug resistance: particular focus on signaling pathways DOI
Raed Obaid Saleh, Mushtak T.S. Al-Ouqaili,

Eyhab Ali

et al.

Medical Oncology, Journal Year: 2024, Volume and Issue: 41(2)

Published: Jan. 9, 2024

Language: Английский

Citations

25
Utilizing non-coding RNA-mediated regulation of ATP binding cassette (ABC) transporters to overcome multidrug resistance to cancer chemotherapy DOI
Kenneth K.W. To,

Zoufang Huang,

Hang Zhang

et al.

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 73, P. 101058 - 101058

Published: Jan. 19, 2024

Language: Английский

Citations

22
Botox-A induced apoptosis and suppressed cell proliferation in fibroblasts pre-treated with breast cancer exosomes DOI Creative Commons

Hossein Sayaf,

Niloufar Salimian,

Mahnaz Mohammadi

et al.

Molecular and Cellular Probes, Journal Year: 2025, Volume and Issue: 79, P. 102007 - 102007

Published: Jan. 6, 2025

Language: Английский

Citations

2
LINC01234 promoted malignant behaviors of breast cancer cells via hsa-miR-30c-2-3p/CCT4/mTOR signaling pathway DOI Creative Commons
Chuangang Tang, Changwen Li,

Chengling Chen

et al.

Taiwanese Journal of Obstetrics and Gynecology, Journal Year: 2024, Volume and Issue: 63(1), P. 46 - 56

Published: Jan. 1, 2024

Despite continuous progress in treatment, recurrence and metastasis limit further improvement the prognosis of breast cancer (BC) patients. Our aim was to search for a crucial prognostic biomarker BC. Patient data were selected from The Cancer Genome Atlas (TCGA) GTEx databases. Several online public databases, including Gene Expression Profiling Interactive Analysis (GEPIA), miRWalk, miRDB, LncBase Predicted v.2, used identify potential upstream miRNAs lncRNAs. These findings validated through vitro experiments. A total 1, 097 invasive BC samples 572 normal tissues (including 113 TCGA 459 GTEx) collected study. CCT4 not only significantly overexpressed compared with but also had important significance (P < 0.001). By intersecting miRWalk miRDB conducting correlation analysis, hsa-miR-30c-2-3p identified as most probable miRNA CCT4. Following an extensive assessment that included survival common binding-site prediction, LINC01234 chosen likely lncRNA. In experiments showed LINC01234-siRNA inhibited proliferation, invasion, migration abilities cells. Western blot analysis confirmed promoted malignant behaviors cells via CCT4/mTOR signaling pathway. LINC01234/hsa-miR-30c-2-3p/CCT4/mTOR axis ceRNA regulatory mechanism established foundation systematically unveiling pathological mechanisms provided new insights targeted therapy

Language: Английский

Citations

7
Molecular Insights on Signaling Cascades in Breast Cancer: A Comprehensive Review DOI Open Access
Venketesh K. Panda,

Barnalee Mishra,

Samikshya Mahapatra

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(2), P. 234 - 234

Published: Jan. 13, 2025

The complex signaling network within the breast tumor microenvironment is crucial for its growth, metastasis, angiogenesis, therapy escape, stem cell maintenance, and immunomodulation. An array of secretory factors their receptors activate downstream cascades regulating cancer progression metastasis. Among various pathways, EGFR, ER, Notch, Hedgehog pathways have recently been identified as in terms proliferation, survival, differentiation, maintenance CSCs, failure. These mediate such MAPK, including MEK/ERK that promote common pro-oncogenic signaling, whereas dysregulation PI3K/Akt, Wnt/β-catenin, JAK/STAT activates key oncogenic events drug resistance, CSC enrichment, metabolic reprogramming. Additionally, these orchestrate an intricate interplay between stromal cells, immune cells. Metabolic reprogramming adaptations contribute to aggressive are unresponsive therapy. Herein, recent insights into novel operating TME aid advancement emphasized current developments practices targeting enhance treatment efficacy reviewed.

Language: Английский

Citations

1
Glycolysis-related lncRNA FTX upregulates YAP1 to facilitate colorectal cancer progression via sponging miR-215-3p DOI Creative Commons

Jin-Lan Yang,

Jingjing Ma,

Tianyin Qu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 22, 2025

Increased evidence reveals that glycolysis is one of the key metabolic hallmarks cancer cells. However, roles lncRNA FTX in energy metabolism and progression remain unclear. In this study we aim to show was significantly upregulated tissues serum CRC patients cell lines. Function indicated it could promote aerobic glycolysis, proliferation, migration invasion colorectal Further mechanistic studies showed, found function as a sponge for miR-215-3p, which reduced ability miR-215-3p repress YAP1 oncoprotein. Additionally, negative correlation observed between expression, knockdown or overexpression yielded opposite effects. conclusion, demonstrates directly combine with competitive endogenous RNA, thus promoting vitro vivo.

Language: Английский

Citations

1
The role and function of autophagy through signaling and pathogenetic pathways and lncRNAs in ovarian cancer DOI Open Access
Seyedsaber Mirabdali, Kimia Ghafouri,

Yalda Farahmand

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 253, P. 154899 - 154899

Published: Oct. 20, 2023

Language: Английский

Citations

11
miR-23b-3p, miR-126-3p and GAS5 delivered by extracellular vesicles inhibit breast cancer xenografts in zebrafish DOI Creative Commons
Iulia Andreea Pelisenco, Daniela Zizioli, Flora Guerra

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 18, 2024

Language: Английский

Citations

4
Long Non-Coding RNAs in Malignant Human Brain Tumors: Driving Forces Behind Progression and Therapy DOI Open Access

Dakun Pei,

Dandan Zhang, Yan Guo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 694 - 694

Published: Jan. 15, 2025

Long non-coding RNAs (lncRNAs) play a pivotal role in regulating gene expression and are critically involved the progression of malignant brain tumors, including glioblastoma, medulloblastoma, meningioma. These lncRNAs interact with microRNAs (miRNAs), proteins, DNA, influencing key processes such as cell proliferation, migration, invasion. This review highlights multifaceted impact lncRNA dysregulation on tumor underscores their potential therapeutic targets to enhance efficacy chemotherapy, radiotherapy, immunotherapy. The insights provided offer new directions for advancing basic research clinical applications tumors.

Language: Английский

Citations

0