Clinical Effectiveness of Physical training incorporating Tuina therapy in Patients with Amyotrophic lateral sclerosis: study protocol for a randomized controlled trial DOI Creative Commons
Dan Yang, Qiaochu Zhu, Yan Zhao

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 20, 2023

Abstract Background: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease, as the limitations of drug therapies, there an increasing focus on rehabilitation approach that includes Physical training (PT) and Tuina therapy. This study aims to investigate protective effects PT therapy physical function, Lung quality life in patients with ALS. Methods: randomized controlled trial. Patients will be divided into experimental group or control at 1:1 ratio, each both have oral Riluzole. The conduct for 4 weeks, while instructed perform basic stretching exercises upper lower limbs home weeks. ALS Functional Rating Scale-Revised scores (ALSFRS-R), Manual muscle test (MMT), Modified Ashworth spasm scale (MAS), Forced vital capacity (FVC), Vital (VC), expiratory volume one second (FEV1), FEV1/FVC, Peak flow (PEF), Maximal voluntary ventilation (MVV), Assessment Questionnaire (ALSAQ-40) scores) safety measures collected baseline, after during follow-ups every 6 months 2 years. Discussion: provide evidence-based medical corroboration clinical application incorporating promote development this Comprehensive early rehabilitation. Trial registration: Chinese trial registry, ChiCTR2300068650. Date: 2023-02-27. https://www.chictr.org.cn/showproj.html?proj=186359

Language: Английский

25-Hydroxycholesterol modulates synaptic vesicle endocytosis at the mouse neuromuscular junction DOI
Eva A. Kuznetsova,

Guzalia F. Zakirjanova,

Andrei N. Tsentsevitsky

et al.

Pflügers Archiv - European Journal of Physiology, Journal Year: 2025, Volume and Issue: 477(3), P. 421 - 439

Published: Jan. 9, 2025

Language: Английский

Citations

2
Modulation of ER-mitochondria tethering complex VAPB-PTPIP51: Novel therapeutic targets for aging-associated diseases DOI Creative Commons
Tao Jiang,

Nan Ruan,

Pengcheng Luo

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 98, P. 102320 - 102320

Published: May 6, 2024

Aging is a gradual and irreversible natural process. With aging, the body experiences functional decline, effects amplify vulnerability to range of age-related diseases, including neurodegenerative, cardiovascular, metabolic diseases. Within aging process, morphology function mitochondria endoplasmic reticulum (ER) undergo alterations, particularly in structure connecting these organelles known as mitochondria-associated membranes (MAMs). MAMs serve vital intracellular signaling hubs, facilitating communication between ER when regulating various cellular events, calcium homeostasis, lipid metabolism, mitochondrial function, apoptosis. The formation partly dependent on interaction vesicle-associated membrane protein-associated protein-B (VAPB) protein tyrosine phosphatase-interacting protein-51 (PTPIP51). Accumulating evidence has begun elucidate pivotal role VAPB-PTPIP51 tether initiation progression In this study, we delineate intricate multifunctional discuss its profound implications aging-associated Moreover, provide comprehensive overview potential therapeutic interventions pharmacological agents targeting VAPB-PTPIP51-mediated MAMs, thereby offering glimmer hope mitigating processes treating disorders.

Language: Английский

Citations

7
Mechanism of Purinergic Regulation of Neurotransmission in Mouse Neuromuscular Junction: The Role of Redox Signaling and Lipid Rafts DOI
А. R. Giniatullin,

Kamilla A. Mukhutdinova,

Alexey M. Petrov

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(8), P. 2021 - 2037

Published: May 30, 2024

Language: Английский

Citations

6
Effects of membrane cholesterol-targeting chemicals on skeletal muscle contractions evoked by direct and indirect stimulation DOI
Nikita S. Fedorov, Artem I. Malomouzh, Alexey M. Petrov

et al.

Journal of Muscle Research and Cell Motility, Journal Year: 2024, Volume and Issue: 45(4), P. 221 - 231

Published: June 21, 2024

Language: Английский

Citations

4
Mechanisms of neuromuscular junction dysfunction in amyotrophic lateral sclerosis DOI Creative Commons
A. N. Khabibrakhmanov, Liaisan A. Akhmadieva, Kerim K. Nagiev

et al.

Annals of Clinical and Experimental Neurology, Journal Year: 2025, Volume and Issue: 19(1), P. 53 - 61

Published: April 3, 2025

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder characterized by the death of upper and lower motor neurons. Numerous studies show that structural functional impairments neuromuscular junctions (NMJ) occur as early presymptomatic stage ALS. NMJ involvement independent one primary events in ALS pathogenesis. Aim: to review data on characteristics mechanisms dysfunction at pre- postsynaptic levels patients transgenic animal model disease. Furthermore, we report perisynaptic Schwann cells impaired neuron skeletal muscle interaction ALS, with focus reviewed publications targeting molecular underlying disruption The may be potential target for novel therapeutic approaches

Language: Английский

Citations

0
Functional and Structural Changes in Diaphragm Neuromuscular Junctions in Early Aging DOI Open Access
Andrei N. Tsentsevitsky, Guzel V. Sibgatullina,

Yulia G. Odoshivkina

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8959 - 8959

Published: Aug. 17, 2024

Age-related impairment of the diaphragm causes respiratory complications. Neuromuscular junction (NMJ) dysfunction can be one triggering events in weaknesses old age. Prominent structural and functional alterations NMJs were described elderly rodents, but NMJ changes middle age remain unclear. Here, we compared muscles from young adult (3 months) middle-aged (12 BALB/c mice. Microelectrode recordings, immunofluorescent staining, electron microscopy, myography, whole-body plethysmography used. We revealed presynaptic (i) postsynaptic (ii) changes. The former included an increase both action potential propagation velocity neurotransmitter release evoked by low-, moderate-, high-frequency activity a decrease immunoexpression synapsin 1 synaptic vesicle clustering. latter consisted currents via nicotinic acetylcholine receptors area their distribution. These correlated with increased contractile responses to moderate- nerve activation. Additionally, found pattern respiration (an peak inspiratory flow tendency elevation tidal volume), which imply conclude that enhancement neuromuscular communication (due mechanism) accompanied improved occurs early aging.

Language: Английский

Citations

3
Oxysterols in Central and Peripheral Synaptic Communication DOI
Alexey M. Petrov

Advances in experimental medicine and biology, Journal Year: 2023, Volume and Issue: unknown, P. 91 - 123

Published: Nov. 30, 2023

Language: Английский

Citations

4
Aberrant evoked calcium signaling and nAChR cluster morphology in a SOD1 D90A hiPSC-derived neuromuscular model DOI Creative Commons

Nathalie Couturier,

Sarah Janice Hörner, Elina Nürnberg

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: June 20, 2024

Familial amyotrophic lateral sclerosis (ALS) is a progressive neuromuscular disorder that due to mutations in one of several target genes, including SOD1 . So far, clinical records, rodent studies, and vitro models have yielded arguments for either primary motor neuron disease, or pleiotropic pathogenesis ALS. While mouse lack the human origin, using induced pluripotent stem cells (hiPSC) been recently developed addressing ALS pathogenesis. In spite improvements regarding generation muscle from hiPSC, degree maturation resulting these protocols has remained limited. To fill shortcomings, we here present new protocol an enhanced myotube differentiation hiPSC with option further upon coculture hiPSC-derived neurons. The described model first yield combination key myogenic features are consistent sarcomeric organization association complex nAChR clusters myotubes derived control hiPSC. this model, carrying D90A mutation had reduced expression markers, sarcomeres, morphologically different clusters, altered nAChR-dependent Ca 2+ response compared myotubes. Notably, trophic support provided by neurons cluster differences between summary, novel yields evidence both muscle-intrinsic nerve-dependent aspects dysfunction -based

Language: Английский

Citations

0
Endocytosis of Synaptic Vesicle in Motor Nerve Endings of FUS Transgenic Mice with a Model of Amyotrophic Lateral Sclerosis DOI
P. N. Grigoryev,

G. A. Gaptrakhmanova,

Anya Plotnikova

et al.

Bulletin of Experimental Biology and Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Language: Английский

Citations

0
Understanding the influence of altered lipid metabolism on TDP-43 pathology: a protocol for a systematic review and meta-analysis v1 DOI Open Access

Tatiana Langerova,

Holly Spence, Fergal M. Waldron

et al.

Published: Dec. 12, 2023

Background: TDP-43 aggregation is the hallmark pathology seen at post-mortem in majority of Amyotrophic lateral sclerosis (ALS) cases. Lipid metabolism has been shown to be disrupted ALS including evidence for systemic metabolic disruptions patients as well lipid peroxidation changes noted single cell level. The temporal nature dysregulation during disease course and role that may play potentiating unclear. Aim: aim this review analyse existing literature assessing interplay between altered ALS, investigate possible relationships two. Methods: This protocol describes strategy a proposed systematic meta-analysis identify, evaluate studies examining relation pathology. Summary: will aid our understanding ALS-associated pathobiology through detailed analysis molecular processes underlying influence lipids on behaviour ALS.

Language: Английский

Citations

1