International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2532 - 2532
Published: Feb. 22, 2024
Cancer immunotherapy is a type of cancer therapy utilizing the immune system to fight against tumors [...]
Language: Английский
Molecular Therapy, Journal Year: 2024, Volume and Issue: 32(5), P. 1266 - 1283
Published: April 2, 2024
Carrier-free naked mRNA vaccines may reduce the reactogenicity associated with delivery carriers; however, their effectiveness against infectious diseases has been suboptimal. To boost efficacy, we targeted skin layer rich in antigen-presenting cells (APCs) and utilized a jet injector. The injection efficiently introduced into cells, including APCs mice. Further analyses indicated that APCs, after taking up antigen skin, migrated to lymph nodes (LNs) for presentation. Additionally, provoked localized lymphocyte infiltration serving as physical adjuvant vaccination. Without carrier, our approach confined distribution site, preventing systemic leakage proinflammatory reactions. In mouse vaccination, elicited robust antigen-specific antibody production over 6 months, along germinal center formation LNs induction of both CD4- CD8-positive T cells. By targeting SARS-CoV-2 spike protein, this provided protection viral challenge. Furthermore, generated neutralizing antibodies non-human primates at levels comparable those observed conclusion, offers safe effective option diseases.
Language: Английский
Citations
15
Vaccines, Journal Year: 2025, Volume and Issue: 13(1), P. 65 - 65
Published: Jan. 13, 2025
Background: Although mRNA vaccines encapsulated in lipid nanoparticles (LNPs) have demonstrated a safety profile with minimal serious adverse events clinical trials, there is opportunity to further reduce reactogenicity. The development of naked could improve vaccine tolerability. Naked nucleic acid delivery using the jet injection method may be solution. Methods: In first part study, optimal conditions providing low traumatization and high expression model mRNA-GFP molecule tissues laboratory animals were determined. Then, we used selected protocol immunize BALB/c mice mRNA-RBD encoding SARS-CoV-2 receptor-binding domain (RBD). It was that vaccinated developed level specific antibodies virus-neutralizing activity. also induced strong RBD-specific T-cell response reduced viral load lungs after infection virus. immune immunized spring-loaded injector comparable LNPs. Results: this efficacy an inexpensive, simple, safe evaluated validated. Conclusions: Our findings suggest possible alternative LNPs for delivering against infection.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 17, 2025
Introduction Needle-free injection system (NFIS) is easy to operate and can decrease needle phobia. Besides, NFIS increase the interaction of antigens in a more dispersed manner with immune cell at local site, which may improve responses mRNA vaccines. Although SARS-CoV-2 vaccines have great success, universal are urgently needed. Delivering by preferred combat COVID-19. Methods RBD3-Fc expressing BA.4, Delta, prototype RBD, human IgG Fc YTE mutation was designed synthesized. The safety naked mRNA-LNP delivered intradermally using (named GV-01) intramuscularly via needles were evaluated compared rats. Results prime-boost regimen administered two routes resulted potent intradermal delivery displays comparable or better performance terms binding antibodies, neutralizing antibodies T responses. Naked functional, but less effective than Discussion above results suggest that safe immunogenic be used as an alternative needles/syringes for inoculation elicit robust systematic
Language: Английский
Citations
0
Inflammopharmacology, Journal Year: 2025, Volume and Issue: unknown
Published: March 19, 2025
Abstract Allergies are a common health issue affecting many people around the world, especially in developed countries. They occur when immune system overreacts to substances that usually harmless. Some allergic conditions include asthma, sinus infections, skin rashes, food allergies, hay fever, severe reactions, eczema, swelling, and reactions medications or insect stings. The causes of these allergies complex often linked genetics, which can lead heightened responses known as atopy. Throughout history, plant extracts have been used for various purposes, including medicine food. In addition, their bioactive compounds show wide range beneficial effects, such reducing fighting oxidative stress, mast cell stabilizers, lowering inflammation, highlighting potential treating conditions. Flavonoids phenolic commonly anaphylaxis potent anti-inflammatory action. This review aims promote use natural products treatments anaphylaxis. discovery new drugs derived from sources holds significant promise management
Language: Английский
Citations
0
Vaccines, Journal Year: 2025, Volume and Issue: 13(5), P. 469 - 469
Published: April 27, 2025
Background: The high incidence and mortality rates of cancer have kept it at the top research agenda for global healthcare industry, as well put serious economic pressure on families society. It has gradually been recognised that reducing through various interventions combining prevention treatment are key to alleviating burden cancer. Methods: Retrieve summarize literature related delivery methods tumor vaccines, investigate whether these applied clinically or used in clinical trials. Results: there a variety vaccine development, but only very small number studies able make strides towards implementing clinic, which is closely linked drawbacks with means delivery. Conclusions: This review analyses reasons why difficult apply clinic from point view method rather than design vaccine. also describes some not yet vaccines and, considering this conjunction those currently purpose, predicts their prospects future application.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4442 - 4442
Published: May 7, 2025
Vaccines usually contain an adjuvant that activates innate immunity to promote the acquisition of adaptive immunity. Aluminum and lipid nanoparticles have been used for this purpose, but their accumulation or widespread circulation in body can lead adverse effects. In contrast, physical adjuvants, which use energy transiently stress tissues, do not persist exposed tissues cause lasting Herein, we investigate effects intradermal injection endotoxin-free ovalbumin (OVA) protein alone without additional adjuvants using a needle-free pyro-drive jet injector (PJI) on tumor vaccination efficacy. Intradermal OVA PJI significantly increased OVA-specific CD8+ T cell expansion lymph node, although node swelling was much less than when aluminum hydroxide used. The also induced killing activity antibody production showed strong cell-dependent prophylactic antitumor against transplanted E.G7-OVA tumors. particular, fluorescent enhanced its uptake by XCR1+ dendritic cells, ability cross-present extracellular proteins skin draining nodes. addition, expression HMGB1, one potent danger signals whose has reported increase response shear stress. Thus, any induces cell-mediated enhancing into high cross-presentation capacity accompanied stress-induced HMGB1.
Language: Английский
Citations
0
Vaccines, Journal Year: 2024, Volume and Issue: 12(5), P. 538 - 538
Published: May 14, 2024
The development of a safe and effective vaccine against avian influenza A virus (AIV) H5N8 is relevant due to the widespread distribution this in bird population existing potential risk human infection, which can lead significant public health concerns. Here, we developed an experimental pVAX-H5 DNA encoding modified trimer AIV hemagglutinin. Immunization BALB/c mice with using jet injection elicited high titer antibody response (the average ELISA was 1 × 105), generated level neutralizing antibodies T-cell response, as determined by ELISpot analysis. Both liquid lyophilized forms provided 100% protection immunized lethal challenge A/turkey/Stavropol/320-01/2020 (H5N8). results obtained indicate that has good opportunities candidate
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Feb. 27, 2023
Abstract Naked mRNA-based vaccines may reduce the reactogenicity associated with delivery carriers, but their effectiveness has been suboptimal against infectious diseases. Herein, we aimed to enhance efficacy by using a pyro-drive liquid jet injector that precisely controls pressure widely disperse mRNA solution in skin. The injection boosted naked efficiency mouse Mechanistic analyses indicate dendritic cells, upon uptake of antigen skin, migrate draining lymph nodes for presentation. Additionally, activated innate immune responses presumably inducing physical stress, thus serving as adjuvant. From safety perspective, our approach, utilizing mRNA, restricted distribution solely site, preventing systemic pro-inflammatory reactions following vaccination. Ultimately, encoding SARS-CoV-2 spike protein elicited robust humoral and cellular immunity, providing protection infection mice. Furthermore, approach induced plasma activity neutralizing non-human primates, comparable observed mice, no detectable reactogenicity.
Language: Английский
Citations
5
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2532 - 2532
Published: Feb. 22, 2024
Cancer immunotherapy is a type of cancer therapy utilizing the immune system to fight against tumors [...]
Language: Английский
Citations
1