Integrating gene expression analysis and ecophysiological responses to water deficit in leaves of tomato plants DOI Creative Commons
Giovanni Bortolami, Tobias de Werk, Maximilian Larter

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 22, 2024

Soil water deficit (WD) significantly impacts plant survival and crop yields. Many gaps remain in our understanding of the synergistic coordination between molecular ecophysiological responses delaying substantial drought-induced effects on growth. To investigate this synergism tomato leaves, we combined molecular, ecophysiological, anatomical methods to examine gene expression patterns physio-anatomical characteristics during a progressing WD experiment. Four sampling points were selected for transcriptomic analysis based key leaves: 4 5 days after (d-WD), corresponding 10% 90% decrease leaf stomatal conductance; 8 d-WD, wilting point; 10 when air embolism blocks 12% xylem transport. At upregulated genes mostly linked ABA-independent responses, with larger-scale ABA-dependent occurring at d-WD. observed an upregulation heat shock transcription factors, two later (10 d-WD), found strong oxidative stress factors. Finally, that young leaves present stronger dehydration tolerance than mature same drought intensity level, presumably because upregulate related increased callose deposition resulting limiting loss phloem, cell rigidity by modifying wall structures. This dataset will serve as framework future studies aim obtain more holistic response level.

Language: Английский

Exploring the mechanisms of WRKY transcription factors and regulated pathways in response to abiotic stress DOI Creative Commons
Hui Li, Muneer Ahmed Khoso,

Jiabo Wu

et al.

Plant Stress, Journal Year: 2024, Volume and Issue: 12, P. 100429 - 100429

Published: March 12, 2024

The environmental conditions encompassing plants exert a significant impact on their appropriate growth and development. It is of utmost importance to investigate the mechanisms signaling cascades underlying tolerance abiotic stress in order enhance quality crops. Plant development processes are significantly impacted by stresses, which intricately linked surroundings. Plants exhibit prompt genetic metabolic network responses, mostly through networks involving transcription factors that respond stress, including WRKY, MYB, bZIP, AP2/EREBP, NAC. Among these WRKY TFs factors, fulfill pivotal function diverse range responses developmental mechanisms. greatly assist coping with stress. These oversee control several target gene categories active involvement numerous interaction W-box cis-acting elements located promoters genes. This research provides comprehensive analysis response mechanism In addition, we have explored state knowledge TFs' effects plants' such as drought, salt, high temperatures, cold. elucidates intricate molecular govern pathways modulate expression, thereby conferring upon plants. Moreover, summarized involved biotic TFs, hormonal routes like SA JA, aid inducing resistance coordinating defense against pathogens challenges. agricultural sustainability augment crop resilience towards strategies manipulate regulatory need be established.

Language: Английский

Citations

19
Deciphering the role of WRKY transcription factors in plant resilience to alkaline salt stress DOI Creative Commons
Sindho Wagan,

Maqsood Ali,

Muneer Ahmed Khoso

et al.

Plant Stress, Journal Year: 2024, Volume and Issue: 13, P. 100526 - 100526

Published: June 28, 2024

The WRKY gene family consists of unique transcription factors (TFs) found exclusively in plants. These TFs play a crucial role regulating how plants respond to various abiotic stresses, such as saline-alkaline conditions, temperature fluctuations, drought, UV radiation and others. Scientists have been progressively studying the roles mechanisms several plant species, including both model essential agricultural crops. This study focus has emerged due understanding that alkaline saline soil stressors considerably impede global productivity. Multiple research efforts underscored significant biological functions assisting coping with challenges, particularly enhancing their ability withstand alkaline-salt stress. review aims investigate structural capabilities impact on responses salt stresses. Additionally, it seeks elucidate these alleviating diverse biotic stressors. objective this is provide comprehensive insights into current state field importance

Language: Английский

Citations

6
Strigolactones affect the stomatal and transcriptomic memory of repeated drought stress in tomato DOI Creative Commons
Ivan Visentin, Eva Campo,

Diana Davydenko

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100740 - 100740

Published: Jan. 1, 2025

Language: Английский

Citations

0
Transcriptome Analysis and Metabolic Profiling Reveal the Key Regulatory Pathways in Drought Stress Responses and Recovery in Tomatoes DOI Open Access
Jinshuai Shu, Lili Zhang, Guiming Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2187 - 2187

Published: Feb. 11, 2024

Drought stress is a major abiotic factor affecting tomato production and fruit quality. However, the genes metabolites associated with responses to water deficiency rehydration are poorly characterized. To identify functional key metabolic pathways underlying drought recovery, drought-susceptible drought-tolerant inbred lines underwent transcriptomic metabolomic analyses. A total of 332 drought-responsive 491 rehydration-responsive core were robustly differentially expressed in both genotypes. The mainly related photosynthesis–antenna proteins, nitrogen metabolism, plant–pathogen interactions, MAPK signaling pathway. Various transcription factors, including homeobox-leucine zipper protein ATHB-12, NAC 29, heat A-6b-like, may be vital for status. Moreover, 24,30-dihydroxy-12(13)-enolupinol, caffeoyl hawthorn acid, adenosine 5′-monophosphate, guanosine identified genotypes under recovery conditions. combined analysis highlighted importance 38 involved pathways, biosynthesis secondary metabolites, amino acids, ABC transporters stress. Our results provide valuable clues regarding molecular basis tolerance rehydration. data presented herein relevant genetically improving tomatoes enhance tolerance.

Language: Английский

Citations

3
Comparative analysis of physical traits, mineral compositions, antioxidant contents, and metabolite profiles in five cherry tomato cultivars DOI Creative Commons
Shuchao Dong, Jingwen Zhang,

Jiayi Ling

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 194, P. 114897 - 114897

Published: Aug. 10, 2024

Cherry tomatoes (Solanum lycopersicum var. cerasiforme) are cultivated and consumed worldwide. While numerous cultivars have been bred to enhance fruit quality, few studies comprehensively evaluated the quality of cherry tomato cultivars. In this study, we assessed fruits five (Qianxi, Fengjingling, Fushan88, Yanyu, Qiyu) at red ripe stage through detailed analysis their physical traits, mineral compositions, antioxidant contents, metabolite profiles. Significant variations were observed among in terms size, shape, firmness, weight, glossiness, sepal length, with each cultivar displaying unique attributes. Mineral revealed distinct patterns essential trace element accumulation, notable differences calcium, sodium, manganese, selenium concentrations. Fenjingling was identified as a enriched cultivar. Analysis contents highlighted Yanyu particularly rich vitamin C having elevated enzyme activities. Metabolomics total number 3,396 annotated metabolites, showed metabolomics Amino acid Fushan88 possess superior profile, while sweetness tartness assessments indicated that exhibited higher soluble solids (TSS) acidity. Notably, (Fushan88, accumulated significantly levels eugenol α-tomatine, compounds associated undesirable flavors, compared pink (Qianxi Fengjingling). Taken together, our results provide novel insights into nutritional value, flavor-associated metabolites tomatoes, offering knowledge could be implemented for breeding, cultivation, marketing

Language: Английский

Citations

2
Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the ‘Epirus’ Region of Greece upon Consecutive Drought Stress DOI Creative Commons

G. Maniatis,

Eleni Tani, Anastasios Katsileros

et al.

Plants, Journal Year: 2023, Volume and Issue: 13(1), P. 27 - 27

Published: Dec. 21, 2023

Within the framework of preserving and valorizing rich grapevine germplasm Epirus region Greece, indigenous (Vitis vinifera L.) cultivars were characterized assessed for their resilience to abiotic stresses in context climate change. The ‘Debina’ ‘Dichali’ displayed significant differences response drought stress as judged by morpho-physiological analysis, indicating higher tolerance Dichali. Hence, they selected further study aiming identify genetic epigenetic mechanisms possibly regulating adaptability. Specifically, self-rooted heterografted on ‘Richter 110’ rootstock plants subjected two phases with a recovery period between. Gene expression analysis was performed stress-related miRNAs target genes: (a) miRNA159 putative targets, VvMYB101, VvGATA-26-like, VvTOPLESS-4-like (b) miRNA156 gene VvCONSTANS-5. Overall, grafted exhibited than plants, suggesting beneficial rootstock–scion interactions. Comparative revealed differential under repetitive between well plants. an up-regulation most genes examined, which may be associated increased tolerance. Nevertheless, profound down-regulation (a transcriptional co-repressor transcription factors) upon concomitant highlights importance this ‘miRNA-target’ module responsiveness. DNA methylation profiling using MSAP patterns genotypes drought. Further investigations will contribute our understanding underlying stress.

Language: Английский

Citations

4
Alteration in the expression of tomato sucrose transporter gene SlSUT4 modulates sucrose subcellular compartmentation and affects responses of plants to drought stress DOI
Yufei Liang, Shurui Chen, Suhong Liu

et al.

Environmental and Experimental Botany, Journal Year: 2023, Volume and Issue: 215, P. 105506 - 105506

Published: Sept. 16, 2023

Language: Английский

Citations

4
Seed priming using different agents can alleviate salt stress in kenaf (Hibiscus cannabinus L.) by activating antioxidant system and related genes expression DOI

Renxue Li,

Dengjie Luo,

Muzammal Rehman

et al.

Physiology and Molecular Biology of Plants, Journal Year: 2024, Volume and Issue: 30(10), P. 1741 - 1757

Published: Oct. 1, 2024

Language: Английский

Citations

1
Deciphering Drought Resilience in Solanaceae Crops: Unraveling Molecular and Genetic Mechanisms DOI Creative Commons
Xin Pang, Jun Chen, Linzhi Li

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1076 - 1076

Published: Dec. 20, 2024

The Solanaceae family, which includes vital crops such as tomatoes, peppers, eggplants, and potatoes, is increasingly impacted by drought due to climate change. Recent research has concentrated on unraveling the molecular mechanisms behind resistance in these crops, with a focus abscisic acid (ABA) signaling pathways, transcription factors (TFs) like MYB (Myeloblastosis), WRKY (WRKY DNA-binding protein), NAC (NAM, ATAF1/2, CUC2- NAM: No Apical Meristem, CUC2: Cup-shaped Cotyledon), omics approaches. Moreover, transcriptome sequencing (RNA-seq) been instrumental identifying differentially expressed genes (DEGs) crucial for adaptation. Proteomics studies further reveal changes protein expression under conditions, elucidating stress response mechanisms. Additionally, microRNAs (miRNAs) have identified key regulators response. Advances proteomics transcriptomics highlighted proteins that respond stress, offering new insights into tolerance. To address challenge of drought, future should emphasize development drought-resistant varieties through precision breeding techniques gene editing, marker-assisted selection (MAS), integration artificial intelligence. adoption environmentally sustainable cultivation practices, including irrigation use anti-drought agents, improving water-use efficiency crop resilience. International collaboration data sharing will be essential accelerate progress ensure global food security arid conditions. These efforts enable adapt challenges posed change, ensuring their productivity sustainability.

Language: Английский

Citations

1
Integrating Gene Expression Analysis and Ecophysiological Responses to Water Deficit in Leaves of Tomato Plants DOI
Giovanni Bortolami, Tobias de Werk, Maximilian Larter

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 5, 2024

Abstract Soil water deficit (WD) is one of the most important abiotic stresses affecting plant survival and crop yield. Despite its economic relevance, many gaps remain in our understanding how crops respond to WD, especially concerning synergistic coordination molecular ecophysiological adaptations delaying damage mortality. In this study, we investigated gene expression imposed by a progressive WD combined it with measurements pointing key thresholds leaves tomato plants. We uncovered transcriptomic changes mature at four stages defined physiological markers relating different intensities: partial stomatal closure, complete after leaf wilting, beginning embolism development veins. By identifying transcription factors (TFs) across these progressively worsening stages, timing impact ABA-(in)dependent regulatory pathways during WD. addition, compared transcriptome young developing versus explored mechanisms that may explain higher tolerance dehydration younger leaves. correlating precise measurements, dataset will serve as framework for future studies comparing responses specific intensities. Highlight Integrated analyses identify underlying

Language: Английский

Citations

0