Oridonin ameliorates ocular surface inflammatory responses by inhibiting the NLRP3/caspase-1/GSDMD pyroptosis pathway in dry eye

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 245, P. 109955 - 109955

Published: June 5, 2024

Language: Английский

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Oral Intake of Streptococcus thermophilus iHA318 Mitigates Dry Eye Symptoms in a Randomized Clinical Study

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 931 - 931

Published: April 10, 2025

Background/Objectives: A probiotic Streptococcus thermophilus (iHA318) has been demonstrated to alleviate dry eye symptoms in a mouse model. This study investigated its effects on mitigation clinical trial. Methods: total of 68 volunteers were recruited the double-blind trial and randomly divided into group placebo group. The received iHA318 capsules daily for 35 days via oral intake, while microcrystalline cellulose capsules. Assessments before after intervention performed tear volume (TV), break-up time (TBUT), osmolarity (Osmo), serum sialic acid (SA) concentrations, Ocular Surface Disease Index (OSDI), an impression cytology analysis was conducted immunofluorescence detection NLRP3 expression. Results: significantly increased group, although effect observed showed significant reduction osmolarity, extended TBUT, improved OSDI score. These parameters also without statistical significance. In addition, SA contrast slight non-significant increase Reductions inflammasome activation found only Conclusions: conclusion, improvement major treatment compared

Language: Английский

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Consumption of Limosilactobacillus fermentum Inhibits Corneal Damage and Inflammation in Dry Eye Disease Mouse Model through Regulating the Gut Microbiome

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3528 - 3528

Published: March 20, 2024

The present study investigated the effect of orally administered Limosilactobacillus fermentum HY7302 (HY7302) on relationship between ocular tissue and microbiome in a corneal injury dry eye mouse model. Specifically, 0.1% benzalkonium chloride (BAC) was applied to surface for 14 days induce male Balb/c mice. During BAC treatment period, (1 × 108 CFU/kg/day or 1 109 CFU/kg/day) an omega-3 positive control (400 mg/kg/day) were (n = eight/group). To examine signaling pathways affected by treatment, vitro effects tight junctions inflammatory response colon epithelial cell line, CMT-93. exposure decreased tear production, induced inflammation detachment, altered gut microbiota. However, oral administration restored secretion detachment BAC-treated Further, alleviated via modulation matrix metalloproteinase-9 (MMP-9) expression affeted alterations microbiota composition. These findings suggest that gut-eye axis interaction affects disease severity injury, alteration could improve health regulating response.

Language: Английский

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Alleviative effect of probiotics and prebiotics on dry eye in type 2 diabetic mice through the gut-eye axis

The Ocular Surface, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Consumption of Limosilactobacillus fermentum Inhibits Corneal Damage and Inflammation in Dry Eye Disease Mice Model through Regulating the Gut Microbiome

Published: Feb. 21, 2024

(1) The present study investigated the effect of orally administered Limosilactobacillus fermentum HY7302 (HY7302) on relationship between ocular tissue and microbiome in corneal injured dry eye mice model. (2) 0.1% benzalkonium chloride (BAC) was applied to surface for 14 days induce injury male Balb/c mice. During BAC treatment period, (1 × 108 CFU/kg/day or 1 109 CFU/kg/day) omega-3 positive control (200 mg/kg/day) were (n = 8/group). To examine signaling pathways affected by treatment, vitro effects tight junctions inflammatory response a mouse colon epithelial cell line, CMT-93. (3) exposure decreased tear production, induced inflammation detachment, altered gut microbiota. However, oral administration restored secretion detachment BAC-treated Further, alleviated via modulation matrix metalloproteinase-9 (MMP-9) expression alterations microbiota composition. (4) These findings suggest that gut–eye axis interaction affects disease severity injury, alteration could improve health regulating response.

Language: Английский

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Streptococcus thermophilus iHA318 Improves Dry Eye Symptoms by Mitigating Ocular Surface Damage in a Mouse Model

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1306 - 1306

Published: June 27, 2024

Dry eye is a complicated ocular surface disease that causes discomfort, visual disturbance, and frequently observed damage. Emerging hypotheses suggest probiotics may help relieve dry symptoms by modulating inflammation oxidative stress. This study aimed to investigate the therapeutic effects of

Language: Английский

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Recommendations for nutritional supplements for dry eye disease: current advances

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 30, 2024

Dry eye disease (DED) represents a prevalent ocular surface disease. The development of effective nutritional management strategies for DED is crucial due to its association with various factors such as inflammation, oxidative stress, deficiencies in polyunsaturated fatty acids (PUFAs), imbalanced PUFA ratios, and vitamin insufficiencies. Extensive research has explored the impact oral supplements, varying composition dosage, on symptoms DED. main components these supplements include fish oils (Omega-3 acids), vitamins, trace elements, phytochemical extracts. Beyond well-known nutrients, it necessary explore whether novel nutrients might contribute more management. This review provides comprehensive update therapeutic potential presents new perspectives combination treatment.

Language: Английский

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Combining MSC Exosomes and Cerium Oxide Nanocrystals for Enhanced Dry Eye Syndrome Therapy

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(9), P. 2301 - 2301

Published: Sept. 11, 2023

Dry eye syndrome (DES) is a prevalent ocular disorder involving diminishe·d tear production and increased evaporation, leading to discomfort potential surface damage. Inflammation reactive oxygen species (ROS) have been implicated in the pathophysiology of DES. one core cause DES vicious cycle. Moreover, there are ROS that regulate inflammation cycle from upstream, which leads treatment failure current therapies merely target inflammation. In this study, we developed novel therapeutic nanoparticle approach by growing cerium oxide (Ce) nanocrystals situ on mesenchymal stem cell-derived exosomes (MSCExos), creating MSCExo-Ce. The combined properties MSCExos aim "inflammation-ROS-injury" pathological mechanism We hypothesized would provide new option for patients with Our analysis confirmed successful crystallization onto MSCExos, MSCExo-Ce displayed excellent biocompatibility. vitro vivo experiments demonstrated promotes corneal cell growth, scavenges ROS, more effectively suppresses compared alone. also ability alleviate symptoms reverse alterations at both cellular tissue levels. conclusion, our findings highlight as promising candidate

Language: Английский

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Exosomes from Limosilactobacillus fermentum Ameliorate Benzalkonium Chloride-Induced Inflammation in Conjunctival Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12282 - 12282

Published: Nov. 15, 2024

Dry eye is characterized by persistent instability and decreased tear production, which are accompanied epithelial lesions inflammation on the surface of eye. In our previous paper, we reported that supplementation with Limosilactobacillus fermentum HY7302 (HY7302) could inhibit corneal damage in a benzalkonium chloride (BAC)-induced mouse model dry eye, through its effects gut microbiome regulation. The aim this study was to determine what functional extracellular substances can alter inflammatory response conjunctival cells. We isolated exosomes from probiotic culture supernatant, analyzed their morphological characteristics, found average size 143.8 ± 1.1 nm, smaller than L. KCTC 3112 strain. addition, HY7302-derived significantly reduced levels genes encoding pro-inflammatory cytokines, including interleukin (IL)-20, IL-8, IL-6, IL-1B, BAC-treated human Moreover, increased tight junction proteins, TJP1, TJP2, occludin-1, Caco-2 Lastly, mRNA expression IL1B, IL20, IL6, IL8, NFAT5 transwell coculture system. Our findings indicate have potential for use treatment ocular inflammation-related disease, gut–eye axis communication via exosomes.

Language: Английский

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Silica-induced ROS in alveolar macrophages and its role on the formation of pulmonary fibrosis via polarizing macrophages into M2 phenotype: A review

Toxicology Mechanisms and Methods, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Sept. 3, 2024

Alveolar macrophages (AMs), the first line against invasion of foreign invaders, play a predominant role in pathogenesis silicosis. Studies have shown that inhaled silica dust is recognized and engulfed by AMs, resulting production large amounts silica-induced reactive oxygen species (ROS), including particle-derived ROS macrophage-derived ROS. These change microenvironment AMs where macrophage phenotype stimulated to swift from M0 M1 and/or M2, ultimately emerge as M2 trigger This complex process accompanied various molecular biological events. Unfortunately, detailed processes mechanisms not been systematically described. In this review, we introduce induced AMs. Then, describe mechanism M2-type polarization caused Finally, review pulmonary fibrosis polarized We conclude initiate fibrotic silicosis inducing into phenotype, targeted intervention can reprogram ameliorate

Language: Английский

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