Ovarian Cell Accumulation of Model PVC Nanoplastics Labelled With CdSe‐QDs Investigated by X‐Ray Fluorescence Microscopy DOI Creative Commons
Alessandra Gianoncelli,

Cristina Tufoni,

Milan Žižić

et al.

X-Ray Spectrometry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

ABSTRACT The distribution of micro‐ and nanoplastics (MNPs) in the environment is increasingly becoming a cause concern for human health. small size these particles makes them prone to accumulate not only tissues various organs but also enables enter cells act as carriers external materials microbes. Since environmental pollutants influence both male female reproductive function foetal development, it expected that this applies MNPs, they can easily organs. This highlights potential risks associated with MNPs need further research area. In system, ovary plays crucial role producing oocytes. SK‐OV‐3 ovarian cancer cell line represents an epithelial‐like model cells, has been widely used nanomedicine nanotoxicological studies. present study, x‐ray fluorescence (XRF) microscopy was investigate accumulations polyvinyl chloride (PVC) (NPs), labelled cadmium‐selenide quantum dots (CdSe‐QDs), at sub‐micrometric spatial resolution. Cd Se QDs components Cl PVC were successfully track NPs confirm do leach QDs, while endogenous elements such Na, Mg, S P monitored biochemical changes attributed toxicity. Our study suggests made even if appear less incline than other types macrophages. Moreover, our results demonstrate XRF analyses are useful tool biological studies aimed deepening understanding on toxicological mechanisms fertility.

Language: Английский

Effects of microplastics on the immune system: How much should we worry? DOI Creative Commons
Claudia Vanetti,

Martina Broggiato,

Stefania Pezzana

et al.

Immunology Letters, Journal Year: 2025, Volume and Issue: 272, P. 106976 - 106976

Published: Feb. 1, 2025

Plastics are everywhere. It is widely recognized that they represent a global problem, the extent of which yet to be defined. Humans broadly exposed plastics, whose effects and consequences poorly characterized so far. The main route exposure via alimentary respiratory intake. pollutions may come from both: water food contamination itself, their packaging. smaller sizes (i.e. microplastics <150 µm - MPs) considered most pervasive living organisms and, therefore, potentially harmful. As humans occupy one apex positions chain, we bioaccumulation biomagnification MPs. In fact, MPs commonly found in human stools blood. However, there no data available on ability accumulate produce detrimental biological systems. Even though plastics pollution studied mammals, including humans, appear have inflammatory effects, rather concerning as many etiologies disease based pro-inflammatory status.

Language: Английский

Citations

0
Mechanism of Nano‐Microplastics Exposure‐Induced Myocardial Fibrosis: DKK3‐Mediated Mitophagy Dysfunction and Pyroptosis DOI
Liang Xiong,

Ziyi Xiong,

Juan Hua

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 22, 2025

ABSTRACT Nano‐microplastics (NMPs), as environmental pollutants, are widely present in nature and pose potential threats to biological health. This study aims investigate the mechanisms by which NMPs inhibit mitophagy through suppression of dickkopf‐related protein 3 (DKK3) expression, leading NOD‐like receptor family, pyrin domain containing (NLRP3) inflammasome‐mediated cardiomyocyte pyroptosis promoting myocardial fibrosis. Healthy adult male C57BL/6 mice were administered NMP solution via gavage, their cardiac function was monitored. The results showed that exposure significantly reduced left ventricular ejection fraction (LVEF) fractional shortening (LVFS) increased extent Transcriptome sequencing identified 14 differentially expressed genes (DEGs), including MYL7. Using random forest algorithm functional enrichment analysis, DKK3 a key gene. In Vitro experiments further confirmed downregulate thereby inhibiting pyroptosis. elucidates molecular induce fibrosis provides new theoretical bases targets for diagnosis treatment heart diseases.

Language: Английский

Citations

0
Long-Term Exposure to Microplastics Promotes Early-Stage Hepatocarcinogenesis Induced by Diethylnitrosamine in Rats by Modulation of Their Gut Microbiota DOI Creative Commons

Huina Guo,

Jianan Wang,

Shaowen Huang

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(5), P. 353 - 353

Published: April 29, 2025

Hepatocarcinogenesis is linked to environmental factors, with microplastics (MPs) emerging as a global concern that may contribute liver injury. However, the impact of MPs on early stages hepatocarcinogenesis has been largely ignored. Here we investigated long-term MP exposure formation preneoplastic lesions during induced by diethylnitrosamine (DEN) in rats. Rats were injected DEN induce lesions, and then they orally administered 1 µm 0.5 mg/kg body weight per day for 20 weeks. The results revealed did not glutathione S-transferase placental form (GST-P)-positive foci these animals, thereby indicating non-carcinogenicity. resulted 1-fold increase both number size GST-P-positive rats initiated compared those treated alone. Accordingly, led 0.61-fold index proliferating cell nuclear antigen (PCNA)-positive cells DEN-initiated when treatment In addition, composition gut microbiota was significantly altered, accompanied various levels short-chain fatty acids. Our suggest can promote pre-neoplastic lesion DEN-induced increased proliferation well alterations acid levels. This highlights potential health risks associated MPs.

Language: Английский

Citations

0
Nanopore detection of sub-nanosized plastics in PE-coated paper cups and analysis of their inflammatory responses DOI
Geonho Cho, Kidong Kim, Weihsuan Chen

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 495, P. 153407 - 153407

Published: June 22, 2024

Language: Английский

Citations

2
Mapping micro(nano)plastics in various organ systems: their emerging links to human diseases? DOI
Yating Luo,

Xiuya Xu,

Qin Yin

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118114 - 118114

Published: Dec. 1, 2024

Language: Английский

Citations

2
Adverse Effects of Micro- and Nanoplastics on Humans and the Environment DOI Open Access
Elena Niccolai, Ilaria Colzi, Amedeo Amedei

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 15822 - 15822

Published: Oct. 31, 2023

The pervasive pollution caused by nano- and microplastics (N/MPLs) is a pressing concern, was exacerbated during the COVID-19 pandemic due to substantial release of disposable Personal Protective Equipment (PPE) into environment [...].

Language: Английский

Citations

4
An Overview of the Detrimental Effect of Microplastics on Humans DOI
Subhankar Das,

Manjula Ishwara Kalyani

Emerging contaminants and associated treatment technologies, Journal Year: 2024, Volume and Issue: unknown, P. 463 - 498

Published: Jan. 1, 2024

Language: Английский

Citations

1
Nano-Sized Polystyrene Plastics Toxicity: Necroptosis Pathway Caused by Autophagy Blockade and Lysosomal Dysfunction DOI

Haiyan Wu,

Huihui Bao, Chaoyu Zhou

et al.

Published: Jan. 1, 2024

The recurrent identification of nano-sized plastic particles in humans, animals, and animal-derived products highlights their potential impact on biological systems. Accordingly, the toxicological profile these has become a critical area investigation. In this study, NP was synthesized successfully with an average particle size 100 nm using emulsion polymerization method as model particles. Following co-incubation IEC-6 cells for 24-168 h, notable inhibition cell viability proliferation observed. significant activation autophagy concomitant blockage autophagic flux after 24-72 hours were unveiled by transmission electron microscopy, western blotting, double-fluorescent analysis. A increase number lysosomes expression hydrolase CTSB detected, indicating dysregulation lysosomal function. Integrative transcriptomic metabolomic analyses, alongside detection activated engagement RIPK1-RIPK3-MLKL/PYGL pathway, suggest that obstruction ensuing dysfunction, which leads to membrane permeabilization (LMP) subsequent necroptosis, is key contributory mechanism cytotoxic effects NP.

Language: Английский

Citations

0
A systems perspective of terrestrial, aquatic, and human health impacts of non-polystyrene-based nanoplastics DOI
Gabriella McConnel, Zachery A Kasuske, Carlos Mazariegos-Ortíz

et al.

Current Opinion in Environmental Science & Health, Journal Year: 2024, Volume and Issue: 39, P. 100557 - 100557

Published: May 11, 2024

Language: Английский

Citations

0
Antitumour effects of SFX-01 molecule in combination with ionizing radiation in preclinical and in vivo models of rhabdomyosarcoma DOI Creative Commons
Simona Camero, Luisa Milazzo, Francesca Vulcano

et al.

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 8, 2024

Abstract Background Despite a multimodal approach including surgery, chemo- and radiotherapy, the 5-year event-free survival rate for rhabdomyosarcoma (RMS), most common soft tissue sarcoma in childhood, remains very poor metastatic patients, mainly due to selection proliferation of tumour cells driving resistance mechanisms. Personalised medicine-based protocols using new drugs or targeted therapies combination with conventional treatments have potential enhance therapeutic effects, while minimizing damage healthy tissues wide range human malignancies, several clinical trials being started. In this study, we analysed, first time, antitumour activity SFX-01, complex synthetic d, l-sulforaphane stabilised alpha-cyclodextrin (Evgen Pharma plc, UK), used as single agent irradiation, four preclinical models alveolar embryonal RMS. Indeed, SFX-01 has shown promise studies its ability modulate cellular pathways involved inflammation oxidative stress that are essential be controlled cancer treatment. Methods RH30, RH4 (alveolar RMS), RD JR1 (embryonal RMS) cell lines well mouse xenograft RMS were evaluate biological molecular effects induced by Flow cytometry modulation key markers analysed q-PCR Western blot assess proliferation, apoptosis, autophagy production intracellular reactive oxygen species (ROS) exposed SFX-01. The migrate invade was also investigated specific assays. possible synergistic between ionising radiation (IR) studied both vitro vivo studies. Student’s t-test two-way ANOVA test statistical significance two more comparisons, respectively. Results treatment exhibited cytostatic cytotoxic mediated G2 cycle arrest, apoptosis induction suppression autophagy. Moreover, able inhibit formation 3D tumorspheres monotherapy IR. Finally, when orally administered agent, displayed pattern efficacy at reducing growth masses models; combined radiotherapy regime, it observed act synergistically, resulting positive outcome than would expected adding each exposure alone. Conclusions summary, our results provide evidence properties tumours, standalone irradiation. These forthcoming findings crucial deeper investigations mechanisms against setting up patients order use SFX-01/IR co-treatment promising approach, particularly management aggressive disease.

Language: Английский

Citations

0