Advances and Challenges of Bioassembly Strategies in Neurovascular In Vitro Modeling: An Overview of Current Technologies with a Focus on Three-Dimensional Bioprinting DOI Open Access

Salvatore Mancuso,

Aditya Bhalerao, Luca Cucullo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11000 - 11000

Published: Oct. 12, 2024

Bioassembly encompasses various techniques such as bioprinting, microfluidics, organoids, and self-assembly, enabling advances in tissue engineering regenerative medicine. Advancements bioassembly technologies have enabled the precise arrangement integration of cell types to more closely mimic complexity functionality neurovascular unit (NVU) that other biodiverse multicellular structures. In this context, bioprinting offers ability deposit cells a spatially controlled manner, facilitating construction interconnected networks. Scaffold-based assembly strategies provide structural support guidance cues for growth, formation complex bio-constructs. Self-assembly approaches utilize inherent properties drive spontaneous organization interaction neuronal vascular components. However, recreating intricate microarchitecture functional characteristics tissue/organ poses additional challenges. materials hold great promise addressing these The further refinement technologies, improved resolution incorporation multiple types, can enhance accuracy biological constructs; however, developing bioinks growth cells, viability, while maintaining compatibility with process remains an unmet need field, advancements design bioactive biodegradable scaffolds will aid controlling adhesion, differentiation, vascularization within engineered tissue. Additionally, integrating advanced imaging analytical real-time monitoring characterization bioassembly, aiding quality control optimization. While challenges remain, ongoing research technological propel field forward, paving way transformative developments engineering. This work provides overview advancements, challenges, future perspectives fabricating constructs add-on focus on technologies.

Language: Английский

Advances in tumor microenvironment: Applications and challenges of 3D bioprinting DOI Creative Commons
Yingying Dong, Xue Zhou, Yunyi Ding

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 730, P. 150339 - 150339

Published: July 8, 2024

The tumor microenvironment (TME) assumes a pivotal role in the treatment of oncological diseases, given its intricate interplay diverse cellular components and extracellular matrices. This dynamic ecosystem poses serious challenge to traditional research methods many ways, such as high costs, inefficient translation, poor reproducibility, low modeling success rates. These challenges require search for more suitable accurately model TME, emergence 3D bioprinting technology is transformative an important complement these precisely control distribution cells, biomolecules, matrix scaffolds within TME. Leveraging digital design, enables personalized studies with precision, providing essential experimental flexibility. Serving critical bridge between vitro vivo studies, facilitates realistic culturing cancer cells. comprehensive article delves into cutting-edge developments bioprinting, encompassing methodologies, biomaterial choices, various models. Exploration current challenges, including limited options, printing accuracy constraints, ethical considerations, contributes nuanced understanding. Despite holds immense potential simulating tissues, propelling medicine, constructing high-resolution organ models, marking trajectory research.

Language: Английский

Citations

4
The utilisation of biliary organoids for biomedical applications DOI Creative Commons

Zhongwen Lei,

Yijun Yang, Yang Xiang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 7, 2025

Biliary duct injury, biliary atresia (BA), tract tumors, primary sclerosing cholangitis (PSC), and other diseases are commonly encountered in clinical practice within the digestive system. To gain a better understanding of pathogenesis development these explore more effective treatment methods, organoid technology has recently garnered significant attention. Organoids three-dimensional structures derived from stem/progenitor cells that can faithfully mimic intricate structure physiological function tissues or organs vitro. They provide valuable platform for studying offer novel possibilities repairing regenerating injuries. The main seed used to construct organoids include human epithelial as well pluripotent stem cells. construction involves various techniques such traditional embedding technology, rotary culture hanging drop along with emerging approaches like organ chip (3D) printing four-dimensional (4D) technology. This article comprehensively reviews methods while discussing their applications disease modeling research on mechanisms drug screening tissue/organ repair; it also highlights current challenges suggests future directions regarding which will serve references treating common refractory system practice.

Language: Английский

Citations

0
An overview of recent progress in cultured meat: focusing on technology, quality properties, safety, industrialization and public acceptance DOI
Yunting Xie, Linlin Cai, Shijie Ding

et al.

Journal of Nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Novel, low-cost bioreactor for in vitro electrical stimulation of cardiac cells DOI Creative Commons
Joseph P. Licata, Jonathan A. Gerstenhaber, Peter I. Lelkes

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 3, 2025

Introduction The successful implantation of laboratory-grown cardiac tissue requires phenotypically mature cardiomyocytes capable electrophysiological integration with native heart tissue. Pulsed electrical stimulation (ES) has been identified as a promising strategy for enhancing cardiomyocyte maturation. However, there are discrepancies in the literature to best practices promoting differentiation using ES. Methods This study presents novel, 3D printed bioreactor that delivers vitro ES human induced pluripotent stem cell-derived (hiPSC-CMs), cell maturity and functional readiness implantation. Finite element analysis mathematical modeling were used model fluid dynamics characterize detail delivery pulsatile signals, providing precise control over parameters such voltage, current, charge. Results developed here provides an easy-to-use, inexpensive platform culturing hiPSC-CMs under influence low-shear flow enhanced nutrient availability, while its “drop-in” design facilitates real-time observation cultured cells. provided is controlled, modeled, predictable, enabling reproducible experimental conditions comparability across future studies. Human (hiPSC-CMs) grown showed improved ability respond external pacing signals. Discussion By offering standardized ES-based maturation, this aims accelerate advancements engineering. Future research will explore how variations phenotype contributing deeper understanding development optimization therapeutic applications.

Language: Английский

Citations

0
Advances in bioinspired polymer hydrogel systems with biomedical functionalities DOI Creative Commons
Kazuhíko Ishihara

Science and Technology of Advanced Materials, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 3, 2025

The concepts of bioinspiration and biomimetics that seek to elucidate the morphology functions living organisms specific reactions within cells, extraction important elements from these design functional molecules high-performance materials are becoming more widespread. This review summarizes progress in research on hydrogels inspired by stimuli-responsiveness cell functions. For application a self-regulated release system insulin regulate blood glucose levels, various polymer have been designed using bioactive such as enzymes lectins sense concentrations. In addition, fully synthetic glucose-responsive hydrogel, complex having phenylboronic acid groups form reversible bonds with sugars multivalent hydroxyl group has researched. hydrogel can be further developed act an extracellular matrix which cells preferably reside. proliferation differentiation encapsulated controlled changes properties response sugar. Another advantage is safely retrieved adding sugar dissociate hydrogel. These bioinspired serve for development new medical technologies, molecules, regulated culture environmental matrices, applications layered three-dimensional systems create organized tissue structures.

Language: Английский

Citations

0
Scalable Matrigel‐Free Suspension Culture for Generating High‐Quality Human Liver Ductal Organoids DOI Creative Commons

Senyi Gong,

Kangxin He, Yu Liu

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Liver transplantation is currently the sole definitive treatment option for end-stage liver failure. However, a significant shortage of donors prevails due to high clinical demands. Recently, human organoids have shown potential in regenerative medicine diseases. Nevertheless, current static cultures grown well-plates heavily rely on extracellular matrix hydrogels (Matrigel), thereby limiting both scalability and quantity organoid culture. In this study, we present groundbreaking culture mode that eliminates all reliance hydrogels, enabling successful preparation functional ductal (LDOs) based cell suspension mechanically stirred bioreactor. Initially, developed 6-well plate without matrigel was proven support robust growth with an average size 2.6 times larger than those obtained culture, survival rate exceeding 90%. Also, transcriptome profile reveals activates phosphatidylinositol 3-kinase (PI3K) signalling pathway through mechanical signal transduction, promoting hepatobiliary characteristics. Then, controllable scalable bioprocess successfully scaled up 50 mL flask bioreactor working volume 15 mL. Finally, animal experiments indicated harvested from can effectively alleviate injury inflammation, demonstrating feasibility large-scale production cultivated improved environment.

Language: Английский

Citations

0
The Role of Biophysical Factors in Organ Development: Insights from Current Organoid Models DOI Creative Commons
Yofiel Wyle,

Nathan Lu,

Jason Hepfer

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(6), P. 619 - 619

Published: June 18, 2024

Biophysical factors play a fundamental role in human embryonic development. Traditional vitro models of organogenesis focused on the biochemical environment and did not consider effects mechanical forces developing tissue. While most tissue has Young’s modulus low kilopascal range, standard cell culture substrate, plasma-treated polystyrene, 3 gigapascals, making it 10,000–100,000 times stiffer than native tissues. Modern approaches attempt to recapitulate biophysical niche organs have yielded more clinically relevant Since Clevers’ conception intestinal organoids 2009, field expanded rapidly, generating stem-cell derived structures, which are transcriptionally similar fetal tissues, for nearly every organ system body. For this reason, we conjecture that will make their first clinical impact regenerative medicine as structures generated ex vivo better match Moreover, autologously sourced transplanted tissues would be able grow with embryo dynamic, environment. As organoid technologies evolve, resultant approach structure function adult may help bridge gap between preclinical drug candidates approved therapeutics. In review, discuss roles stiffness, viscoelasticity, shear formation disease development, suggesting these physical parameters should further integrated into improve physiological relevance therapeutic applicability. It also points mechanotransductive Hippo-YAP/TAZ signaling pathway key player interplay extracellular matrix cellular mechanics, pathways. We conclude by highlighting how frontiers physics can applied biology, example, quantum entanglement predict spontaneous DNA mutations. future, contemporary theories leveraged understand seemingly stochastic events during organogenesis.

Language: Английский

Citations

3
iPSC-derived and Patient-Derived Organoids: Applications and challenges in scalability and reproducibility as pre-clinical models DOI Creative Commons

Elisa Heinzelmann,

Francesco Piraino,

Mariana Costa

et al.

Current Research in Toxicology, Journal Year: 2024, Volume and Issue: 7, P. 100197 - 100197

Published: Jan. 1, 2024

Language: Английский

Citations

3
Emerging brain organoids: 3D models to decipher, identify and revolutionize brain DOI
Yuli Zhao, Ting Wang,

Jiajun Liu

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 378 - 402

Published: Feb. 12, 2025

Language: Английский

Citations

0
Organoids in Dynamic Culture: Microfluidics and 3D Printing Technologies DOI
Xin Su, Mingqi Wang,

Ruqiang Yuan

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

With the rapid advancement of biomaterials and tissue engineering technologies, organoid research its applications have made significant strides. Organoids are increasingly utilized in pharmacology, regenerative medicine, precision clinical medicine. Current trends moving toward multifunctional composite three-dimensional cultivation dynamic strategies. Key technologies driving this evolution, including 3D printing microfluidics, continue to impact new areas discovery relevance. This review provides a systematic overview these emerging trends, discussing strengths limitations critical offering insight directions for professionals working field.

Language: Английский

Citations

0