Establishment of regeneration, transformation, and genome editing procedures for a seed-propagated carnation (Dianthus caryophyllus L.) variety DOI Creative Commons

ZhongJian Li,

Masafumi Yagi, Ryohei Koyama

et al.

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2024, Volume and Issue: 160(1)

Published: Dec. 30, 2024

Abstract Carnations ( Dianthus caryophyllus L.) are amongst the three most commercially valuable cut flowers worldwide. However, traditional breeding methods often time-consuming and labor-intensive. Although genome editing is used as an alternative method for creating new varieties, high heterozygosity of carnations inhibits ability to maintain varietal characteristics in null segregants except target-derived traits. The use homozygous lines a possible solution. Therefore, this study aimed establish regeneration, transformation, using seed-carnation varieties. effects four auxins (indole-3-butyric acid, IBA; a-naphthaleneacetic NAA; 2,4-dichlorophenoxyacetic 2,4-D; 3-indoleacetic IAA) five cytokinins (6-benzyladenine, BA; thidiazuron, TDZ; kinetin, KT; zeatin, ZT; N 6 -2-isopentenyl adenine, 2IP) on callus shoot induction were evaluated. combination 0.05 mg/l 2,4-D 4 TDZ had highest formation rate at 28%. In addition, hyperhydricity was reduced by increasing size culture vessels. Sucrose, agar, AgNO 3 concentrations, well pH, optimized facilitate regeneration. Hygromycin 12.5 subsequently selection agent after Agrobacterium -mediated transformation. Finally, phytoene desaturase gene knocked out CRISPR/Cas9 system. obtained albino one-base deletion or two-base insertion sequence. To our knowledge, first system callus-derived shoots from seed-propagated carnation, which may contribute rapid

Language: Английский

Harnessing haploid‐inducer mediated genome editing for accelerated maize variety development DOI Creative Commons
Lina Li,

Xiao Fu,

Xiantao Qi

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Summary The integration of haploid induction and genome editing, termed HI‐Edit/IMGE, is a promising tool for generating targeted mutations crop breeding. However, the technical components stacking suitable maize seed industry have yet to be fully characterised tested. Here, we developed assessed three HI‐Edit/IMGE lines: Edit Wx , Sh & using inducer CHOI3 lines engineered CRISPR‐Cas9 system targeting Waxy1 ( Wx1 ) Shrunken2 Sh2 genes. We meticulously systems, focusing on copy numbers mutant alleles mtl dmp which facilitate induction. Using B73 six other parental major commercial varieties as recipients, demonstrated maternal efficiencies ranging from 8.55% 20.89% mutation rates between 0.38% 1.46%. Comprehensive assessment verified identification, target gene editing accuracy, background integrity, related agronomic traits. Notably, successfully combined distinct systems induce multiple desired mutations, highlighting potential in accelerating edited traits into varieties. Our findings underscore importance meticulous Cas9 number characterisation highlight challenges somatic chimerism. also validated performance single‐cross haploids derived process. results confirm industrial applicability through pollination provide critical insights further optimising this technology.

Language: Английский

Citations

1
Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species DOI
Hannah Levengood, Yun Zhou, Cankui Zhang

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(11)

Published: Oct. 29, 2024

Language: Английский

Citations

4
Simplex and multiplex CRISPR/Cas9‐mediated knockout of grain protease inhibitors in model and commercial barley improves hydrolysis of barley and soy storage proteins DOI Creative Commons
Michael Panting, Inger Bæksted Holme, Giuseppe Dionisio

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Anti-nutritional factors in plant seeds diminish the utilization of nutrients feed and food. Among these, protease inhibitors inhibit protein degradation by exogenous proteases during digestion. Through conventional selection-gene-free genome editing using ovules as explants, we used simplex multiplex CRISPR/Cas9 for studying impact chymotrypsin inhibitor CI-1A, CI-1B CI-2, Bowman-Birk trypsin inhibitor, Serpin-Z4, barley ɑ-amylase/subtilisin on soybean storage degradation. Mutants were generated commercial cultivar Stairway, having a high level inhibition, model Golden Promise, lower inhibition level. In all individual knockouts decreased three α-chymotrypsin, Ronozyme ProAct significantly. The triple knockout further α-chymotrypsin proteases. Degradations recombinant proteins B- C-hordeins significantly improved following mutagenesis. single CI-1A almost compares to effect achieved promise, uncovering major that cultivar. Stairway mutant demonstrated hordeins glycinin β-conglycinin. results this study provide insights into cereal genes their negative effects most important from soybeans. suggests future focus target improving food digestibility.

Language: Английский

Citations

0
Histological Aspects of a Long-Term In Vitro Culture of Morphogenic Calluses of Lavandula angustifolia Mill. DOI
Н. Н. Круглова, O.A. Seldimirova,

А.Е. Zinatullina

et al.

Biology Bulletin, Journal Year: 2025, Volume and Issue: 52(1)

Published: Feb. 1, 2025

Language: Английский

Citations

0
Genome editing in Sub-Saharan Africa: a game-changing strategy for climate change mitigation and sustainable agriculture DOI Creative Commons

Peter Amoah,

Abdoul-Razak Oumarou Mahamane, Moise Hubert Byiringiro

et al.

GM crops & food, Journal Year: 2024, Volume and Issue: 15(1), P. 279 - 302

Published: Oct. 31, 2024

Sub-Saharan Africa's agricultural sector faces a multifaceted challenge due to climate change consisting of high temperatures, changing precipitation trends, alongside intensified pest and disease outbreaks. Conventional plant breeding methods have historically contributed yield gains in Africa, the intensifying demand for food security outpaces these improvements confluence factors, including rising urbanization, improved living standards, population growth. To address escalating demands amidst growth, paradigm shift toward more sustainable innovative crop improvement strategies is imperative. Genome editing technologies offer promising avenue achieving sustained increases while bolstering resilience against biotic abiotic stresses associated with change. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein (CRISPR/Cas) unique its ubiquity, efficacy, precision, making it pivotal tool African improvement. This review highlights challenges explores prospect gene secure region's future foods.

Language: Английский

Citations

3
Recalcitrance to transformation, a hindrance for genome editing of legumes DOI Creative Commons

V. M. Nivya,

Jasmine M. Shah

Frontiers in Genome Editing, Journal Year: 2023, Volume and Issue: 5

Published: Sept. 21, 2023

Plant genome editing, a recently discovered method for targeted mutagenesis, has emerged as promising tool crop improvement and gene function research. Many genome-edited plants, such rice, wheat, tomato, have over the last decade. As preliminary steps in procedure editing involve genetic transformation, amenability to depends on efficiency of engineering. Hence, there are numerous reports aforementioned crops because they transformed with relative ease. Legume rich protein and, thus, favored source plant proteins human diet most countries. However, legume cultivation often succumbs various biotic/abiotic threats, thereby leading high yield loss. Furthermore, certain legumes like peanuts possess allergens, these need be eliminated deprive many people from gaining benefits crops. Further variations limited legumes. Genome potential offer solutions not only combat stress but also generate desirable knock-outs variants. excluding soybean, alfalfa,

Language: Английский

Citations

7
Marker-Assisted Breeding Techniques for the Development of Gluten-Free Wheat Varieties: A Comprehensive Review DOI Open Access

Sanjay V Bennur,

Mukh Ram,

Shalini Shalini

et al.

Journal of Advances in Biology & Biotechnology, Journal Year: 2024, Volume and Issue: 27(5), P. 256 - 267

Published: April 6, 2024

Ingestion of gluten proteins from wheat, barley and rye poses a significant health risk. The sole treatment for various disease is lifelong adherence to strict gluten-free diet. Conventional breeding methods have limitations in producing wheat varieties that maintain baking quality due the complexity genome multitude genes. This review explores use RNA interference (RNAi) silencing CRISPR/Cas9 gene editing techniques. Efficient screening selection processes identifying lines with reduced celiac epitopes at both DNA protein levels, as well maintaining quality, are discussed. integration production products holds potential meeting growing demand safer dietary options while ensuring compliance regulatory standards addressing complex challenges associated management. Marker-assisted (MAS) offers several advantages over conventional plant breeding, including timesaving, cost-effectiveness goal-oriented outcomes. aims delineate molecular markers, encompassing sequence-tagged sites (STS), simple sequence repeats (SSR), genotyping by sequencing (GBS), single nucleotide polymorphism (SNP) arrays, exome capture, Competitive Allele Specific PCR (KASP), cleaved amplified polymorphic sequences (CAPS), semi-thermal asymmetric reverse (STARP), target (GBTS). Additionally, we compile quantitative trait loci (QTL)/genes their which hold utility MAS applications. rapid advancement genomics poised expedite marker development, QTL mapping, cloning, processes.

Language: Английский

Citations

1
PIF4 and phytohormones signalling under abiotic stress DOI Creative Commons

Hina Arya,

Mohan B. Singh, Prem L. Bhalla

et al.

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: unknown, P. 106016 - 106016

Published: Oct. 1, 2024

Language: Английский

Citations

1
MORPHOHISTOLOGICAL PECULIARITIES OF RHIZOGENESIS IN VITRO IN WHEAT EMBRYONIC CALLUSES DOI Open Access
A.E. Zinatullina

ÈKOBIOTEH, Journal Year: 2023, Volume and Issue: 7(1), P. 15 - 26

Published: April 26, 2023

In modern biotechnological studies of economically valuable plants, numerous developments aimed at obtaining calluses are presented, in which various pathways and types morphogenesis induced vitro. At the same time, such type as rhizogenesis vitro, consisting formation development roots, but without further regeneration has not been studied sufficiently. Nevertheless, it is important to identify tissue mechanisms roots structures. Using example obtained from immature wheat embryos, was revealed that morphogenetic foci basis Anatomical characteristics emerging have revealed, largely coincide with similar cereal under planta conditions. It proposed use morphogenic vitro model systems for studying intact plants.

Language: Английский

Citations

1
Genome engineering in barley DOI

Hajra Maqsood,

Arzoo Ahad,

Somia Khan

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 257 - 272

Published: Jan. 1, 2024

Citations

0