Biology,
Journal Year:
2024,
Volume and Issue:
13(7), P. 463 - 463
Published: June 21, 2024
Breast
cancer
is
the
most
prevalent
among
women
worldwide.
Therapeutic
strategies
to
control
tumors
and
metastasis
are
still
challenging.
Three-dimensional
(3D)
spheroid-type
systems
more
accurately
replicate
features
of
in
vivo,
working
as
a
better
platform
for
performing
therapeutic
response
analysis.
This
work
aimed
characterize
epithelial-mesenchymal
transition
doxorubicin
(dox)
mammary
tumor
spheroid
(MTS)
model.
We
evaluated
treatment
effect
on
MCF-7
diameter,
cell
viability,
death,
migration
proteins
involved
(EMT)
process.
Spheroids
were
also
produced
from
formed
4T1
67NR
lines.
MTSs
mimicked
avascular
characteristics,
exhibited
adherens
junction
independently
their
own
extracellular
matrix.
Our
model
supports
3D
culturing
cells
isolated
mice
tumors.
Through
assay,
we
verified
reduction
E-cadherin
expression
an
increase
vimentin
became
distant
spheroids.
Dox
promoted
cytotoxicity
inhibited
EMT
These
results
suggest,
first
time,
that
this
reproduces
aspects
process
describes
potential
dox
inhibiting
metastatic
process,
which
can
be
further
explored.
Biochemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 19, 2025
In
vitro
cytotoxicity
assays
are
critical
tools
for
assessing
the
potential
toxicity
of
compounds
in
early-stage
drug
discovery,
toxicology,
and
biomedical
research.
These
help
evaluate
effects
chemical,
pharmaceutical,
biological
agents
on
cellular
health
viability.
However,
reliability
data
is
often
compromised
by
a
variety
experimental
pitfalls.
This
chapter
discusses
key
factors
that
can
lead
to
inaccurate
or
misleading
results
provides
guidelines
avoiding
them.
Common
issues
such
as
inappropriate
assay
selection,
interference
from
serum
components
like
fetal
bovine
(FBS),
cell
density
variations,
incorrect
incubation
times
highlighted.
The
importance
using
appropriate
controls,
solvent-induced
toxicity,
preventing
compound
aggregation
also
emphasized.
By
optimizing
conditions
understanding
limitations
each
type,
researchers
enhance
precision
their
tests.
aims
provide
comprehensive
guide
improving
design,
ensuring
reproducibility,
generating
reliable
be
confidently
applied
development
toxicological
evaluations.
Journal of Functional Biomaterials,
Journal Year:
2025,
Volume and Issue:
16(3), P. 74 - 74
Published: Feb. 20, 2025
Head
and
neck
squamous
cell
carcinoma
(HNSCC)
presents
significant
challenges
in
oncology
due
to
its
complex
biology
poor
prognosis.
Traditional
two-dimensional
(2D)
culture
models
cannot
replicate
the
intricate
tumor
microenvironment,
limiting
their
usefulness
studying
disease
mechanisms
testing
therapies.
In
contrast,
three-dimensional
(3D)
vitro
provide
more
realistic
platforms
that
better
mimic
architecture,
mechanical
features,
cellular
interactions
of
HNSCC.
This
review
explores
properties
3D
developed
for
HNSCC
research.
It
highlights
key
techniques,
such
as
spheroids,
organoids,
bioprinted
tissues,
emphasizing
ability
simulate
critical
characteristics
like
hypoxia,
drug
resistance,
metastasis.
Particular
attention
is
given
stiffness,
elasticity,
dynamic
behavior,
highlighting
how
these
emulate
native
tissues.
By
enhancing
physiological
relevance
studies,
offer
potential
revolutionize
research
facilitate
development
effective,
personalized
therapeutic
strategies.
bridges
gap
between
preclinical
clinical
applications
by
summarizing
providing
guidance
developing
systems
both
biological
advancing
innovation
cancer
therapy.
Lab on a Chip,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Polymer-based
microwell
platforms
have
garnered
much
interest
due
to
their
usefulness
in
culturing
and
analyzing
small
quantities
of
biological
cells
spheroids.
Existing
methods
for
fabricating
polymer
arrays
involve
complex
fabrication
processes
and/or
are
limited
ability
create
dense
very
(<50
μm
diameter)
microwells.
Here,
we
present
a
simple
rapid
technique
high-density
microwells
ranging
from
20
160
diameter
on
variety
substrates.
In
this
approach,
surface
is
ablated
using
CO2
laser
that
rastered
over
stainless
steel
mesh,
which
serves
as
shadow
mask.
A
theoretical
laser-polymer
interaction
model
was
developed
predicting
the
volume
based
substrate
properties
settings.
Microwell
volumes
predicted
by
were
within
5.4%
fabricated
determined
experimentally.
Cellulose
acetate
used
culture
Lewis
lung
carcinoma
expressing
ovalbumin
(LLC-OVA),
maintained
up
72
h
with
negligible
(<5%)
loss
viability.
As
second
proof
principle
demonstration,
LLC-OVA
grown
co-cultured
OT-I
T
measurements
interferon
gamma
(IFN-γ),
marker
cell
activation,
performed
revealed
positive
correlation
between
cell-T
time
activation.
These
two
vitro
demonstrations
showcase
capability
generating
high-throughput
cellular
studies,
including
growth
dynamics
studies
studies.
Furthermore,
envision
these
can
be
different
types
other
applications,
such
spheroid
formation
single
analysis,
further
expanding
utility
technique.
PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(3), P. e0319610 - e0319610
Published: March 11, 2025
The
discovery
of
novel
anti-leishmanial
compounds
is
essential
due
to
the
limitations
current
treatments
and
lack
new
drugs
in
development.
In
this
study,
we
employed
Quasi
Vivo
900
medium
perfusion
system
(QV900,
Kirkstall
Ltd,
UK)
simulate
physiological
fluid
flow,
allowing
us
compare
macrophage
responses
therapeutic
outcomes
under
dynamic
versus
static
conditions.
After
24
hours,
phagocytosis
macropinocytosis
decreased
all
cell
types
flow
conditions
compared
cultures.
Under
slow
(1.45
x
10
-9
m/s)
faster
(1.23
-7
((simulating
vivo
lymphatic
flow),
by
around
42.55%
56.98%
peritoneal
macrophages
(PEMs),
42.21%
56.11%
bone
marrow-derived
(BMMs),
49.75%
63.32%
THP-1
cells,
respectively.
Similarly,
approximately
40.7%
62.2%
PEMs,
34.8%
60.9%
BMMs,
33.3%
59.3%
line
same
further
assessed
impact
on
drug
efficacy
functions
using
a
Leishmania
major
amastigote-macrophage
assay.
We
evaluated
performance
both
standard
nanoparticle-based
formulations
within
culture
systems.
72
hours
perfusion,
chitosan
solution,
blank
chitosan-sodium
tripolyphosphate
(TPP)
nanoparticles,
amphotericin
B
(AmB)-loaded
chitosan-TPP
nanoparticles
exhibited
statistically
significant
reduction
antileishmanial
activity
30-50%
60-80%
comparison,
pure
AmB
showed
40%
decrease
at
67%
significant.
These
results
highlighted
importance
considering
dynamics
vitro
studies
for
more
accurate
simulation
conditions,
potentially
leading
better
strategies
cutaneous
leishmaniasis
(CL).
Abstract
A
‘gut-brain
axis’
is
an
intricate
bidirectional
connection
between
the
gut
and
central
nervous
system,
serving
as
a
key
pathway
for
signal
exchange.
However,
current
in
vitro
models
do
not
fully
capture
dynamics
of
interactions
these
organs,
which
limits
mechanistic
understanding
therapeutic
exploration.
Here,
we
present
3D
human
gut-brain
vascular
(GBV)
model
that
simulates
communication
entities,
allowing
us
to
investigate
disorders
originating
from
both
brain.
We
created
physiologically
relevant
axis
model,
creating
villus-like
lumenized
barrier,
blood
vascular-astrocyte
interactions,
brain
tissue
mimics
neurovascular
interactions.
Next,
demonstrated
gut-to-brain
signaling
by
introducing
bacterial-derived
toxins
into
side,
penetrate
barrier
ultimately
reaching
leading
tauopathy,
indicator
neurodegeneration.
observed
brain-to-gut
exposing
side
risk
factors
Alzheimer’s
(AD)
Parkinson’s
(PD),
induces
neuroinflammation,
disrupts
subsequently
affects
epithelial
integrity.
Our
precisely
engineered
microphysiological
system
emulates
gut-brain-vascular
serves
translational
tool
identify
targets
evaluate
pharmaceuticals
neurological
gastrointestinal
disorders.
ACS Omega,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 18, 2025
The
surge
in
antibiotic-resistant
Staphylococcus
aureus
infections
has
been
deemed
a
major
public
health
concern.
There
is
an
urgent
need
for
novel
antimicrobial
therapies,
chemical
and
nonantibiotic.
basidiomycota-derived,
secondary
metabolite
pleurotin
shown
to
be
effective
against
Gram-positive
bacteria,
while
bacteriophages
could
the
ultimate
nonantibiotic
alternative.
In
this
study,
combination
of
phage
K
targeting
S.
was
examined.
Pleurotin
isolated
from
basidiomycota
fungus
Hohenbuehelia
grisea.
cytotoxicity
assessed
two
human
cell
lines
comparison
pleuromutilin,
vancomycin,
K.
antibiotics
were
then
tested
independently
or
with
strains.
Cytotoxicity
cells
comparable
vancomycin
pleuromutilin.
Results
suggest
that
adding
synergistic
effect
can
lower
MIC
pleurotin,
vancomycin.
This
demonstrates
viable
antistaphylococcal
drug.
