Fascial Manual Medicine: The Concept of Fascial Continuum

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Fascial tissue ubiquitously pervades the body system, becoming target of many disciplines that use manual techniques for patient treatment. It is a much-debated topic as there currently no univocal definition among different authors. Due to non-discontinuity fascia, we can speak fascial continuum; this principle basis osteopathic perspective. This vision, which seems banal, not always applied in medicine, where, often, it conditioned by reductionist (layers) and mechanistic (compartments) approach, forgetting machine but an organism. continuity teaches treatment does only reverberate area where operator's hands rest creates series local systemic adaptations. narrative review revises concept continuum highlighting fascia system (different tissues working harmony), multi-organ (capable behaving like organ), whose macroscopic functional expression (movement) microscopic (with cellular adaptations) derives from nanoscopic coherence (electromagnetic behaviors). means acts unit, makes approach never systemic. The aim article highlight fact single biological entity (solid fluid), medicine segment, entire person.

Language: Английский

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Establishment of a patient-derived 3D in vitro meningioma model in xeno-free hydrogel for clinical applications

Acta Neuropathologica Communications, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 24, 2025

Meningiomas exhibit a complex biology that, despite notable successes in preclinical studies, contributes to the failures of pharmaceutical clinical trials. Animal models using patient tumor cells closely mimic vivo conditions but are labor-intensive, costly, and unsuitable for high-throughput testing. In comparison, monolayer cell (two-dimensional, 2D) cost-efficient lack primary cell-cell interactions, potentially overestimating treatment effects. Three-dimensional (3D) offer an alternative through more precise mimicking morphology physiology than 2D less costly methods. Here, we aimed establish 3D model solid xeno-free medium patient-derived tumors, thus creating bench-to-clinic pathway personalized Four WHO grade 1 one 2 (third-passage, fresh) 12 meningioma (sixth-passage, frozen) malignant IOMM-Lee line were used models. The was developed medium. After 3 months 13 days line, extracted assessed histology, immunohistochemistry, epigenetic analyses (EPICv2 array) on five pairs evaluate their structural fidelity, cellular composition, landscape compared original tumor. None frozen samples successfully generated Models from fresh immunohistochemically similar tumors models, particularly regarding proliferation. displayed loss fibrous tissue. All had copy number variation profiles, visually. Genome-wide DNA methylation level patterns between tumors. Correlation plots CpG levels showed high congruency meningiomas corresponding all (R > 0.95). Our mimicked terms morphology, immunohistochemical markers genome-wide patterns, providing cost-effective accessible This approach has potential facilitate strategies patients requiring additional therapy beyond surgery.

Language: Английский

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Conductive biological materials for in vitro models: properties and sustainability implications

In vitro models, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract The integration of conductive biological materials into in vitro models represents a transformative approach to advancing biomedical research while addressing critical sustainability challenges. Traditional used tissue engineering and disease modeling are often environmentally detrimental, derived from non-renewable resources, limited their ability replicate the dynamic properties native tissues. Conductive bridge this gap by offering unique combination biodegradability, sustainability, functional properties, such as bioelectricity biocompatibility, that essential for mimicking physiological environments. Herein, development current applications biodegradable materials, including advanced polymers polyaniline polypyrrole, carbon-based nanocomposites, renewable biopolymers lignin cellulose, overviewed. These not only reduce ecological footprint but also enable precise simulation electrical signaling tissues, cardiac, neural, muscular systems, thereby enhancing relevance models. Their three-dimensional (3D) constructs, organ-on-chip platforms, bioprinting technologies facilitates patient-specific models, paving way personalized therapeutic diagnostic applications. In addition precision, these align with global efforts implement circular economy principles research, promoting resource efficiency waste reduction. By combining environmental responsibility state-of-the-art functionality, redefining future 3D accelerating innovation regenerative medicine, drug development, fostering sustainable framework scientific discovery.

Language: Английский

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Recent Advances in Graphene Oxide-Based on Organoid Culture as Disease Model and Cell Behavior – A Systematic Literature Review

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 6201 - 6228

Published: June 1, 2024

Abstract: Due to their ability replicate the in vivo microenvironment through cell interaction and induce cells stimulate function, three-dimensional culture models can overcome limitations of two-dimensional models. Organoids are 3D that demonstrate natural structure an organ. In most organoid tissue cultures, matrigel made a mouse tumor extracellular matrix protein mixture is essential ingredient. However, its tumor-derived origin, batch-to-batch variation, high cost, safety concerns have limited usefulness drug development regenerative medicine. Its clinical application has also been hindered by fact generation dependent on use poorly defined matrices. Therefore, optimization crucial step developing introduces alternatives as different materials. Recently, variety substitute materials reportedly replaced matrigel. The purpose this study review significance latest advances for applications how they enhance build network systems generating proper behavior. Excellence behavior evaluated from characteristics, proliferation, differentiation, even gene expression. As result, graphene oxide demonstrated potency Graphene promote good well known having biocompatibility. Hence, provide opportunities future advanced Keywords: organoid, culture, graphene, matrigel, alternative behavior, host-pathogen

Language: Английский

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Spheroid Model of Mammary Tumor Cells: Epithelial–Mesenchymal Transition and Doxorubicin Response

Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 463 - 463

Published: June 21, 2024

Breast cancer is the most prevalent among women worldwide. Therapeutic strategies to control tumors and metastasis are still challenging. Three-dimensional (3D) spheroid-type systems more accurately replicate features of in vivo, working as a better platform for performing therapeutic response analysis. This work aimed characterize epithelial-mesenchymal transition doxorubicin (dox) mammary tumor spheroid (MTS) model. We evaluated treatment effect on MCF-7 diameter, cell viability, death, migration proteins involved (EMT) process. Spheroids were also produced from formed 4T1 67NR lines. MTSs mimicked avascular characteristics, exhibited adherens junction independently their own extracellular matrix. Our model supports 3D culturing cells isolated mice tumors. Through assay, we verified reduction E-cadherin expression an increase vimentin became distant spheroids. Dox promoted cytotoxicity inhibited EMT These results suggest, first time, that this reproduces aspects process describes potential dox inhibiting metastatic process, which can be further explored.

Language: Английский

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