Synergistic Proliferation Effects of Xanthohumol and Niflumic Acid on Merkel and Glioblastoma Cancer Cells: Role of Cell Membrane Interactions DOI Open Access
Monika Stompor, Aleksandra Włoch, P. Sengupta

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11015 - 11015

Published: Oct. 13, 2024

The objective of our research was to determine the effects xanthohumol (XN), a flavonoid isolated from hops (Humulus lupulus), and anti-inflammatory drug niflumic acid (NA), separately in combination with each other, on proliferation human cancer cells. Additionally, so as understand mechanism underlying anticancer properties tested compounds, their biophysical parameters model membrane were assessed. cells incubated XN NA at various concentrations, either individually or other. Cell quantified using sulforodamine B (SRB) assay. In addition, IC50 values for applied determined by cell tests following lines: 5637 (urinary bladder carcinoma), A-431 (epidermoid UM-SCC-17A (head neck squamous SK-MEL-3 (melanoma), MCC13 (Merkel cancer), A172 (glioblastoma), comparison mouse normal fibroblasts (BALB/3T3 clone A31). results show that two-compound combinations significantly decreased dose-dependent manner, stronger than additive responses individually. studies revealed synergistic effect rigidity when mixture NA, which may explain observed increase activity combined NA. Our suggest NSAIDs, such acid, be promising strategy co-application drugs.

Language: Английский

Development of a Bioprocess to Improve the Phenolic Compounds Content and Antioxidant Capacity in Blue Corn Grains DOI Creative Commons
Ulises Ramírez-Esparza,

Andrés Javier Ordoñez-Cano,

Emilio Ochoa-Reyes

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(3), P. 122 - 122

Published: March 4, 2025

Corn, a widely cultivated cereal, is rich source of several antioxidant compounds, including phenolic compounds. Some these compounds are bound within the components corn cell wall, requiring specific treatments to release them. Solid-state fermentation (SSF) using Rhizopus oryzae enhanced capacity and content in legumes grains. However, there limited information regarding its application blue corn. This study aimed evaluate effect R. growth on kernels. The results demonstrated that used kernels for growth, which led an increase free reaching their highest concentration at 60 h culture. Compared unfermented control (0 h), SSF increased by up 161% total 94% capacity. main identified extracts RP-HPLC-ESI-MS included caffeic acid 4-O-glucoside, rosmanol, p-coumaroyl tartaric glucosidic ester, 5-heptadecylresorcinol, caffeoyl acid. represents eco-friendly method enhancing grain.

Language: Английский

Citations

0
Arecoline-Induced Hepatotoxicity in Rats: Screening of Abnormal Metabolic Markers and Potential Mechanisms DOI Creative Commons
Jing Sun, Kai Zhang,

Yihui Yin

et al.

Toxics, Journal Year: 2023, Volume and Issue: 11(12), P. 984 - 984

Published: Dec. 4, 2023

Arecoline is a pyridine alkaloid derived from areca nut in the Arecaceae family. It has extensive medicinal activity, such as analgesic, anti-inflammatory, and anti-allergic. However, toxicity of limits its application. Most current studies on mainly focus immunotoxicity, carcinogenesis, cancer promotion. there are few systematic hepatotoxicity mechanisms. Therefore, this research explored mechanism induced by rats analyzed endogenous metabolite changes rat plasma combining network toxicology with metabolomics. The differential metabolites after exposure, D-Lysine, N4-Acetylaminobutanal, L-Arginine, were obtained metabolomics study, these involved regulation lipid metabolism, amino acid vitamin metabolism. Based strategy toxicology, can affect HIF-1 signaling pathway, MAPK PI3K-Akt other concerning pathways regulating critical targets, ALB, CASP3, EGFR, MMP9. Integration results further analyzed, it was concluded that may induce mediating oxidative stress, inflammatory response, energy cell apoptosis.

Language: Английский

Citations

7
Prenanthes purpurea and 3,5-DiCQA Alleviate Cellular Stress in H2O2-Induced Neurotoxicity: An In Vitro Comparative Study DOI Open Access
Rositsa Mihaylova, Dimitrina Zheleva‐Dimitrova,

Viktoria Elincheva

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9805 - 9805

Published: Sept. 11, 2024

Oxidative stress exerts multiple disruptive effects on cellular morphology and function is a major detriment to age-related pathological neurodegenerative processes. The present study introduces an evaluative comparative investigation of the antioxidant cytoprotective properties Prenanthes purpurea extract its constituent 3,5-dicaffeoylquinic acid (DiCQA) in vitro model H2O2-induced neurotoxicity. Using validated silico approaches, we established presence concentration dynamics protection 24 h pretreatment regimen with natural products. conducted cytotoxicity studies automated Chou–Talalay analysis for studying drug interactions demonstrated strong antagonistic effect tested substances against oxidative stimuli “on demand” manner, prevailing at higher end range. These findings were further supported by proteomic characterization treatment samples, accounting more distinct neuroprotection provided pure polyphenol 3,5-DiCQA.

Language: Английский

Citations

1
Synergistic Proliferation Effects of Xanthohumol and Niflumic Acid on Merkel and Glioblastoma Cancer Cells: Role of Cell Membrane Interactions DOI Open Access
Monika Stompor, Aleksandra Włoch, P. Sengupta

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11015 - 11015

Published: Oct. 13, 2024

The objective of our research was to determine the effects xanthohumol (XN), a flavonoid isolated from hops (Humulus lupulus), and anti-inflammatory drug niflumic acid (NA), separately in combination with each other, on proliferation human cancer cells. Additionally, so as understand mechanism underlying anticancer properties tested compounds, their biophysical parameters model membrane were assessed. cells incubated XN NA at various concentrations, either individually or other. Cell quantified using sulforodamine B (SRB) assay. In addition, IC50 values for applied determined by cell tests following lines: 5637 (urinary bladder carcinoma), A-431 (epidermoid UM-SCC-17A (head neck squamous SK-MEL-3 (melanoma), MCC13 (Merkel cancer), A172 (glioblastoma), comparison mouse normal fibroblasts (BALB/3T3 clone A31). results show that two-compound combinations significantly decreased dose-dependent manner, stronger than additive responses individually. studies revealed synergistic effect rigidity when mixture NA, which may explain observed increase activity combined NA. Our suggest NSAIDs, such acid, be promising strategy co-application drugs.

Language: Английский

Citations

1