Exploring the Impact of Apelin and Reactive Oxygen Species on Autophagy and Cell Senescence in Pre-eclampsia DOI
Xue Peng,

Xi Tan,

Li Dai

et al.

Free Radical Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 26

Published: Dec. 23, 2024

This research investigates the interplay between Reactive Oxygen Species (ROS) and Apelin (APLN) in regulating autophagy, with implications for placental cell senescence apoptosis pre-eclampsia (PE). We manipulated APLN expression using sgRNA to study its effects on ROS levels subsequent cellular responses. Our findings reveal that overexpression elevates production, accelerating apoptosis. In contrast, silencing enhances thereby diminishing aging These outcomes were confirmed vitro vivo experiments, establishing a causative relationship ROS-mediated modulation altered dynamics PE. The results suggest potential therapeutic targets within pathways alleviate detrimental changes placenta, offering new strategies clinical management of emphasizes crucial role autophagy health sets stage future investigations into interventions pregnancy-related complications.

Language: Английский

Quercetin suppresses ROS production and migration by specifically targeting Rac1 activation in gliomas DOI Creative Commons

Rafia A. Baba,

Hilal Ahmad Mir,

Taseem A. Mokhdomi

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 31, 2024

P66Shc and Rac1 proteins are responsible for tumor-associated inflammation, particularly in brain tumors characterized by elevated oxidative stress increased reactive oxygen species (ROS) production. Quercetin, a natural polyphenolic flavonoid, is well-known redox modulator with anticancer properties. It has the capacity to cross blood–brain barrier and, thus, could be possible drug against tumors. In this study, we explored effect of quercetin on Rac1/p66Shc-mediated tumor cell which principal pathway generation ROS cells. Glioma cells transfected Rac1, p66Shc, or both were treated varying concentrations different time points. Quercetin significantly reduced viability migration an ROS-dependent manner concomitant inhibition Rac1/p66Shc expression production naïve Rac1/p66Shc-transfected lines, suggestive preventing activation. Through molecular docking simulations, observed that showed best binding compared other known inhibitors specifically blocked GTP-binding site A-loop prevent GTP We conclude exerts its effects via modulation Rac1-p66Shc signaling inhibiting activation, thus restraining growth.

Language: Английский

Citations

7
Autophagy regulates apoptosis of colorectal cancer cells based on signaling pathways DOI Creative Commons
Yuwei Yan, Wenyan Yu, Min Guo

et al.

Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 25, 2024

Colorectal cancer is a common malignant tumor of the digestive system. Its morbidity and mortality rank among highest in world. Cancer development associated with aberrant signaling pathways. Autophagy process cell self-digestion that maintains intracellular environment has bidirectional regulatory role cancer. Apoptosis one important death programs cells able to inhibit development. Studies have shown variety substances can regulate autophagy apoptosis colorectal through pathways, participate regulation on apoptosis. In this paper, we focus relevant research based involvement related pathways order provide new ideas therapeutic directions for treatment

Language: Английский

Citations

4
A sesquiterpene lactone, tomentosin, as a novel anticancer agent: orchestrating apoptosis, autophagy, and ER stress in colorectal cancer DOI Creative Commons
Sümeyra Çetinkaya, Ebru Güçlü, İlknur Çınar Ayan

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Abstract Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide. Natural compounds with anticancer potential, such as tomentosin, sesquiterpene lactone derived from Inula viscosa , are under investigation alternative therapeutic agents. However, its potential effects on CRC remain unexplored. This study aimed to evaluate the tomentosin in cells and elucidate underlying molecular mechanisms. HCT 116 HT- 29 were treated cell viability, colony formation, invasion, apoptosis, mitochondrial membrane (MMP), reactive oxygen species (ROS) production, autophagy, endoplasmic reticulum (ER) stress evaluated. Various assays, including XTT, Matrigel invasion used assess proliferation, invasion. Tomentosin markedly reduced viability formation dose-dependent manner. It suppressed induced evidenced by an increased apoptotic index upregulation CASP3 CASP7 CASP8 CASP9 BAX . disrupted MMP elevated ROS levels, contributing signaling. Autophagic activity was significantly upregulated, expression BECLIN1 ATG5 ATG7 MAP1LC3 A ER markers GRP78 ATF6 CHOP XBP1 also suggesting role death. has inducing modulating triggering stress. These findings underscore tomentosin’s novel candidate for CRC, warranting further vivo clinical investigations.

Language: Английский

Citations

0
Exploring the anti-cancer and Antimetastatic effect of Silymarin Against Lung Cancer. DOI Creative Commons

Srithika Srinivasan,

Aruchamy Mohanprasanth, Ahmed Nadeem

et al.

Toxicology Reports, Journal Year: 2024, Volume and Issue: 13, P. 101746 - 101746

Published: Sept. 28, 2024

Language: Английский

Citations

3
Effect of Acacia concinna Extract on Apoptosis Induction Associated with Endoplasmic Reticulum Stress and Modulated Intracellular Signaling Pathway in Human Colon HCT116 Cancer Cells DOI Open Access

Pornnapa Sitthisuk,

Sukanda Innajak, Watcharaporn Poorahong

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3764 - 3764

Published: Nov. 1, 2024

Colorectal cancer (CRC) stands as one of the most prevalent types and among frequent causes cancer-related death globally.

Language: Английский

Citations

2
Network pharmacology and in vitro experiments reveal sophoridine‐induced apoptosis and G1 phase arrest via ROS‐dependent PI3K/Akt/FoxO3a pathway activation in human bladder cancer cells DOI
Yao Su, Lin Chen, Jin Yang

et al.

Chemical Biology & Drug Design, Journal Year: 2024, Volume and Issue: 103(2)

Published: Feb. 1, 2024

Abstract Bladder cancer (BLCA), a common primary malignancy, exhibits resistance to conventional chemotherapeutic agents. Sophoridine (SR) is quinoline alkaloid derived from the traditional Chinese herb Sophora alopecuroides L., which belongs legume family Sophoraceae. SR reported exert growth‐inhibitory effects against several cancers. However, mechanisms underlying of on BLCA have not been elucidated. This study performed molecular and cellular experiments verify mechanisms. inhibited cell proliferation promoted apoptosis G1‐phase arrest through PI3K/AKT/FoxO3a signaling pathway. More interestingly, can be attributed accumulation reactive oxygen species (ROS) in vivo. ROS may upstream factor this Additionally, migration invasion cells concentration‐dependent or time‐dependent manner. first demonstrate ROS‐dependent pathway‐mediated anticancer effect mechanism BLCA. The correlation between SR‐induced inhibition, apoptosis, cycle arrest, suggests that promising novel therapeutic for

Language: Английский

Citations

1
Design, synthesis, and antiproliferative activity evaluation of novel α-mangostin derivatives by ROS/MAPK signaling pathway DOI

Mangaladoss Fredimoses,

Li Pan, Yunqing Zhang

et al.

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 153, P. 107968 - 107968

Published: Nov. 16, 2024

Language: Английский

Citations

1
A Zeolitic Imidazolate Framework-Based Antimicrobial Peptide Delivery System with Enhanced Anticancer Activity and Low Systemic Toxicity DOI Creative Commons
Jingwen Jiang,

Kaderya Kaysar,

Yanzhu Pan

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1591 - 1591

Published: Dec. 13, 2024

Background: The clinical efficacies of anticancer drugs are limited by non-selective toxic effects on healthy tissues and low bioavailability in tumor tissue. Therefore, the development vehicles that can selectively deliver release at site is critical for further improvements patient survival. Methods: We prepared a CEC nano-drug delivery system, CEC@ZIF-8, with zeolite imidazole framework-8 (ZIF-8) as carrier, which achieve response folate receptor (FR). characterized this system terms morphology, particle size, zeta potential, infrared (IR), x-ray diffraction (XRD), transcriptome analysis, examined vitro cytotoxicity cellular uptake properties CEC@ZIF-8 using cervical cancer cells. Lastly, we established TC-1 tumor-bearing mouse model evaluated its vivo anti-cervical activity. Results: nano-delivery had favorable biocompatibility, heat stability, pH responsiveness, loading efficiency 12%, hydrated size 174 ± 5.8 nm, potential 20.57 mV, slow massive drug an acidic environment (pH 5.5), whereas was 6% neutral 7.4). At same time, confocal imaging cell viability assays demonstrated greater intracellular accumulation more potent against cells compared to free CEC. mechanism analyzed series analyses, revealed NPs differentially regulate expression levels 1057 genes cells, indicated enriched pathways were mainly cycle apoptosis-related via enrichment analysis differential genes. Flow cytometry showed inhibited growth HeLa arresting G0/G1 phase. also induced apoptosis rates than CEC, while unloaded ZIF-8 little inherent pro-apoptotic Furthermore, reactive oxygen species (ROS) upregulated ROS inhibitors caspase reversed NPs-induced apoptosis. Finally, reduced rate xenograft tumors mice without systemic toxicity observed cisplatin treatment. Conclusions: significantly enhanced effect both vitro, providing promising applications management. work demonstrates CEC-loaded nanoparticles selective destruction tissues.

Language: Английский

Citations

1
Bruceantinol works as a CDK2/4/6 inhibitor to inhibit the growth of breast cancer cells DOI Creative Commons
Li Sun, Yumeng Wang, Jia Li

et al.

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 395, P. 110999 - 110999

Published: April 10, 2024

Bruceantinol (BOL), isolated from the dried fruit of Brucea javanica (L.) Merr., exhibits cytotoxic effects on breast cancer cells. However, underlying mechanism remains to be fully addressed. In this paper, MCF-7 and MDA-MB-231 human cell lines were used as experimental models uncover how BOL inhibits growth. The growth, proliferation, cycle, apoptosis investigated using MTT assays, EdU incorporation flow cytometry, respectively. Bioinformatics techniques applied predict key targets in cancer. Subsequent validation these anti-breast was conducted through Western blotting, RT-PCR, siRNA transfection, molecular docking analysis. results demonstrated that dose- time-dependently reduced growth both lines, impeded disrupted induced necrosis cells Furthermore, CDK2/4/6 identified targets, their knockdown sensitivity BOL. found potentially bind with facilitate protein degradation proteasome pathway. Additionally, activated ERK cells, activation required for BOL's functions Collectively, may act an inhibitor exert effects. Its expression also depend HRs

Language: Английский

Citations

0
Exploring the Impact of Apelin and Reactive Oxygen Species on Autophagy and Cell Senescence in Pre-eclampsia DOI
Xue Peng,

Xi Tan,

Li Dai

et al.

Free Radical Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 26

Published: Dec. 23, 2024

This research investigates the interplay between Reactive Oxygen Species (ROS) and Apelin (APLN) in regulating autophagy, with implications for placental cell senescence apoptosis pre-eclampsia (PE). We manipulated APLN expression using sgRNA to study its effects on ROS levels subsequent cellular responses. Our findings reveal that overexpression elevates production, accelerating apoptosis. In contrast, silencing enhances thereby diminishing aging These outcomes were confirmed vitro vivo experiments, establishing a causative relationship ROS-mediated modulation altered dynamics PE. The results suggest potential therapeutic targets within pathways alleviate detrimental changes placenta, offering new strategies clinical management of emphasizes crucial role autophagy health sets stage future investigations into interventions pregnancy-related complications.

Language: Английский

Citations

0