Unveiling Novel Regulatory Mechanisms of miR-5195-3p in Pelvic Organ Prolapse Pathogenesis DOI
Hao Zhang, Xinlu Wang, Meng Dong

et al.

Biology of Reproduction, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Pelvic organ prolapse is a condition that significantly affects women’s quality of life. The pathological mechanism pelvic not yet fully understood, and its pathogenesis often caused by multiple factors, including the metabolic imbalance extracellular matrix. This study aims to investigate role miR-5195-3p, microRNA, in pathology regulatory mechanism. Using various molecular biology techniques such as real-time reverse transcription Polymerase Chain Reaction (PCR), fluorescence situ hybridization, immunohistochemistry, Western blot, miR-5195-3p expression was examined vaginal wall tissues obtained from patients. Results revealed an up-regulation these tissues, showing negative correlation with matrix–related proteins. Further analysis using bioinformatics tools identified Lipoxygenase (LOX) potential target prolapse. Dual luciferase reporter gene experiments confirmed LOX direct miR-5195-3p. Interestingly, regulating also influenced transforming growth factor β1 signaling pathway had impact on matrix metabolism. finding suggests controls metabolism targeting modulating TGF-β1 pathway. In conclusion, this unveils involvement through LOX/TGF-β1 axis. These findings reveal new mechanisms prolapse, providing theoretical foundation therapeutic targets for further research treatment.

Language: Английский

Circular RNA in cancer DOI
Vanessa M. Conn, Arul M. Chinnaiyan, Simon J. Conn

et al.

Nature reviews. Cancer, Journal Year: 2024, Volume and Issue: 24(9), P. 597 - 613

Published: July 29, 2024

Language: Английский

Citations

49
Current advances in circular RNA detection and investigation methods: Are we running in circles? DOI
Rareş Drulă, Cornelia Braicu, Ioana Berindan‐Neagoe

et al.

Wiley Interdisciplinary Reviews - RNA, Journal Year: 2024, Volume and Issue: 15(3)

Published: May 1, 2024

Abstract Circular RNAs (circRNAs), characterized by their closed‐loop structure, have emerged as significant transcriptomic regulators, with roles spanning from microRNA sponging to modulation of gene expression and potential peptide coding. The discovery functional analysis circRNAs been propelled advancements in both experimental bioinformatics tools, yet the field grapples challenges related detection, isoform diversity, accurate quantification. This review navigates through evolution circRNA research methodologies, early detection techniques current state‐of‐the‐art approaches that offer comprehensive insights into biology. We examine limitations existing methods, particularly difficulty differentiating isoforms distinguishing linear counterparts. A critical evaluation various tools novel strategies is presented, emphasizing need for integrated enhance our understanding interpretation functions. Our underscore dynamic rapidly advancing nature research, highlighting ongoing development analytical frameworks designed address complexity facilitate assessment clinical utility. As such, this overview aims catalyze further study, fostering a deeper cellular processes implications disease. article categorized under: RNA Methods > Nanotechnology Analyses Cells In Vitro Silico

Language: Английский

Citations

10
Research progress in the ethnopharmacology, phytochemistry, pharmacology, toxicology, and quality control of Valeriana jatamansi Jones DOI
Zhichao Wang, Yajun Shi, Xiaofei Zhang

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 332, P. 118403 - 118403

Published: May 29, 2024

Language: Английский

Citations

3
The Role of the Dysregulation of circRNAs Expression in Glioblastoma Multiforme DOI
Yafei Wang, Ying Yu, Jiahua Yu

et al.

Journal of Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 75(1)

Published: Jan. 22, 2025

Language: Английский

Citations

0
Exosomal circ_0001583 Drives Glioblastoma Cell Advancement Through the miR-647/CKAP2L Pathway DOI Creative Commons
Yuhao Zhang, Shiming Liu,

Cheng Wu

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Language: Английский

Citations

0
The Role of Endogenous Circular RNAs in Glioblastoma Treatment Resistance: A Literature Review DOI Creative Commons

Sejal Mohapatra

Undergraduate Research in Natural and Clinical Science and Technology (URNCST) Journal, Journal Year: 2024, Volume and Issue: 8(8), P. 1 - 11

Published: Aug. 16, 2024

Introduction: Glioblastomas are a high-grade primary brain cancer of astrocytes, glial cell. The average survival upon diagnosis is 15 months. Researchers have been continuously attempting to develop new drugs and therapies that would exhibit greater efficiency against this cancer. However, tumor resistance often observed in glioblastomas. Circular RNA relatively novel finding non-coding highly stable compared linear RNA. Recent studies found variation circular expression levels resistant versus non-resistant cell lines. Thus, researchers exploring endogenous entity understand the role it may play chemo- radioresistant Methods: A comprehensive look into most recent findings on topic was done using Google Scholar PubMed search engines. Key words used include "circRNA," "glioblastoma," "chemo- radio-resistance". This review mainly stems from research published between 2017 2024. Results: Only few specific RNAs be implicated chemoresistance. Research has focused understanding temozolomide. certain also noted cisplatin histone deacytlase inhibitor for glioblastoma treatment. These inhibit cytotoxic effects chemotherapeutics primarily though microRNA sponging. Additionally, similar results were tumors, whereby block apoptosis by altering various protein mechanisms through sponging inhibiting translocation. Discussion: Developing methods tumor's ability resist otherwise effective an ideal method Many attempted modify cellular pathways facilitate cells. due complex interactions possible off-target effects, these inefficient. serve as improved therapeutic targets their tissue specificity increased stability. Conclusion: newly identified involved therapy increase susceptibility treatments. Knock-out overexpression analysis indicate molecules sufficient abilities Highly targeting techniques therefore viable solution radioresistance.

Language: Английский

Citations

1
The Road to Overcome Pancreatic Cancer: Where Are We? DOI Creative Commons
Alexandru Tirpe, Cristian Streianu, Ekaterina Isachesku

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38196 - e38196

Published: Sept. 20, 2024

Pancreatic cancer (PC) is an intricate malignancy with poor prognosis. In the present state of art review paper and clinical trial trend analysis, we explore current clinically employed pancreatic body knowledge future research directions. When considering PDACs' molecular biology, underline role PanIN in carcinogenesis mutational gain, as well distinctive tumor microenvironment characteristic dense fibrotic stroma. The landscape PC typically involves KRAS, TP53, SMAD4 CDKN2A genes, but other mutations can be identified depending on PDAC subtype. Due to various factors, there are currently limited therapeutic options - from a surgical-centered approach resectable stage, systemic more advanced stages, including potential applicability personalized medicine. Currently, numerous trials undergoing that study landscapes use newer or repurposed chemotherapeutics, introduction immunotherapeutic agents, antibody-drug conjugates, TCR-T cell therapy mDC3/8-KRAS vaccines, among others.

Language: Английский

Citations

0
Unraveling the mechanisms of glioblastoma’s resistance: investigating the influence of tumor suppressor p53 and non-coding RNAs DOI
Qamar A. Alshammari, Saud O. Alshammari, Abdullah S. Alshammari

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Language: Английский

Citations

0
Unveiling Novel Regulatory Mechanisms of miR-5195-3p in Pelvic Organ Prolapse Pathogenesis DOI
Hao Zhang, Xinlu Wang, Meng Dong

et al.

Biology of Reproduction, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Pelvic organ prolapse is a condition that significantly affects women’s quality of life. The pathological mechanism pelvic not yet fully understood, and its pathogenesis often caused by multiple factors, including the metabolic imbalance extracellular matrix. This study aims to investigate role miR-5195-3p, microRNA, in pathology regulatory mechanism. Using various molecular biology techniques such as real-time reverse transcription Polymerase Chain Reaction (PCR), fluorescence situ hybridization, immunohistochemistry, Western blot, miR-5195-3p expression was examined vaginal wall tissues obtained from patients. Results revealed an up-regulation these tissues, showing negative correlation with matrix–related proteins. Further analysis using bioinformatics tools identified Lipoxygenase (LOX) potential target prolapse. Dual luciferase reporter gene experiments confirmed LOX direct miR-5195-3p. Interestingly, regulating also influenced transforming growth factor β1 signaling pathway had impact on matrix metabolism. finding suggests controls metabolism targeting modulating TGF-β1 pathway. In conclusion, this unveils involvement through LOX/TGF-β1 axis. These findings reveal new mechanisms prolapse, providing theoretical foundation therapeutic targets for further research treatment.

Language: Английский

Citations

0