Carpal tunnel syndrome
Nature Reviews Disease Primers,
Journal Year:
2024,
Volume and Issue:
10(1)
Published: May 23, 2024
Language: Английский
Barriers and new opportunities in developing effective therapies for diabetic neuropathy: International expert consensus recommendations
Diabetes Research and Clinical Practice,
Journal Year:
2025,
Volume and Issue:
unknown, P. 112010 - 112010
Published: Jan. 1, 2025
Diabetic
neuropathy
(DN)
affects
up
to
half
of
individuals
with
type
1
and
2
diabetes.
Despite
evidence
that
improving
metabolic
cardiovascular
health
can
slow
its
progression,
DN
remains
a
significant
clinical
challenge
due
the
lack
disease-modifying
therapies
effective
pain
management
strategies.
This
consensus
aimed
identify
gaps
recommend
strategies
address
these
challenges.
A
workshop,
initiated
by
Steno
Diabetes
Centre
Copenhagen
Danish
Endocrinology
Academy,
conducted
gap
analysis
based
on
insights
from
studies,
observational
cohorts,
practice.
Online
invitations
targeted
experienced
clinicians,
researchers,
drug
developers
committed
treatment
through
innovative
trials.
Thirty-five
participants
six
countries
reached
via
Delphi
process
key
steps
advance
therapy.
Four
critical
barriers
needs
were
addressed:
(1)
Translating
bench
research
practice,
(2)
Enhancing
trial
design,
(3)
Improving
outcome
measures,
(4)
Identifying
treatments
for
painful
DN.
Successful
interventional
trials
require
robust
measures
capture
clinically
meaningful
changes
in
phenotypes,
providing
basis
developing
effective,
treatments.
Language: Английский
Refinement of Protein Extraction Protocols for Human Peripheral Nerve Tissue
ACS Omega,
Journal Year:
2025,
Volume and Issue:
10(5), P. 5111 - 5118
Published: Feb. 2, 2025
Our
aim
was
to
establish
an
effective
method
for
protein
extraction
from
freshly
frozen
human
peripheral
nerves,
determine
the
minimum
amount
required
consistent
outcomes,
and
assess
which
produced
highest
number
of
identities.
Five
methods
were
compared
using
8
M
urea
Ripa
buffer
either
Bullet
Blender
or
Bioruptor.
Out
total
2619
identified
proteins,
combined
with
Bioruptor
resulted
in
identification
1582
(60%)
1615
(62%)
respectively.
In
contrast,
1022
proteins
(39%),
whereas
employing
yielded
1446
(55%).
Sample
amounts,
ranging
0.6
10
mg,
prepared
outcome
obtained
samples
≥1.2
mg.
Combining
increased
2126
(81%).
Proteins
classified
by
their
cell
components,
molecular
functions,
biological
processes.
Furthermore,
a
subclassification
involved
extracellular
matrix
(ECM)
introduced.
We
recommend
use
buffer,
combination
extracting
fresh-frozen
nerves
weighing
Language: Английский
Lactoferrin-derived peptide PXL01 impacts nerve regeneration after sciatic nerve reconstruction in healthy and diabetic rats
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
13
Published: April 7, 2025
Although
advanced
surgical
techniques
are
available,
satisfactory
functional
outcomes
after
peripheral
nerve
injuries
uncommon.
Hence,
immune-modulating
factors
such
as
PXL01,
a
lactoferrin-derived
peptide
that
improves
axonal
outgrowth
in
injured
human
digital
nerves,
have
gained
attention.
We
previously
reported
short-term
immunosuppressive
effect
of
PXL01
the
repair
transected
rat
sciatic
but
it
had
no
on
regeneration.
Here,
we
investigated
potential
to
improve
regeneration
healthy
rats
and
model
type
2
diabetes
(Goto-Kakizaki
[GK]
rats).
A
10-mm
defect
was
created
(n
=
14)
diabetic
GK
reconstructed
using
autografts.
Immediately
surgery,
or
sodium
chloride
(control,
placebo)
7
for
each
treatment)
administered
around
autograft.
On
day
8,
immunohistochemical
staining
dorsal
root
ganglia
(DRGs)
performed
analyze
(neurofilament
staining);
inflammation
(CD68
CD206
macrophage
nerve);
Schwann
cell
sensory
neuron
activation
(transcription
factor
ATF3
DRGs)
apoptosis
(cleaved
caspase
3
neuroprotection
(heat
shock
protein
[HSP27]
DRGs).
impact
response
autografts
increased
HSP27
expression
DRGs
rats,
despite
lower
number
activated
cells
Diabetes
affected
outgrowth,
responses,
expression.
These
effects
were
observed
well
DRG.
Application
having
macrophages,
may
affects
autograft-reconstructed
conveys
(HSP27
expression)
rats.
influenced
Therefore,
is
promising
candidate
Language: Английский
DA.Vra1-congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
13
Published: April 28, 2025
The
rat
Vra1
locus,
containing
glutathione
S-transferase
alpha
4
(Gsta4),
regulates
the
degeneration
of
central
nervous
system
(CNS)
neurons
in
toxin-,
protein-,
and
injury-based
models.
We
hypothesize
that
Piebald
Virol
Glaxo.1AV1
(PVG)
alleles
confer
protection
increased
axonal
outgrowth
after
peripheral
nerve
injury
repair.
DA
rats
(n
=
14)
with
PVG
locus
(DA.Vra1,
n
were
subjected
to
sciatic
transection
immediate
After
6
days,
protein
gene
expression
analyzed
injured
uninjured
nerves
dorsal
root
ganglia
(DRG).
No
differences
observed
between
strains,
but
number
apoptotic
Schwann
cells
distal
end
was
higher
DA.Vra1
than
(p
0.003).
In
both
gene-
activating
transcription
factor
3
(ATF3)
27-kDa
heat
shock
(HSP27,
i.e.,
Hspb1)
vs.
DRG.
rats,
Gsta4
lower
DRG
0.043)
0.008)
0.008).
had
Atf3
0.016)
caspase
0.032)
rats.
Results
highlight
complexity
repair,
supporting
further
investigation
regeneration.
Language: Английский
Serum levels of biomarkers related to severity staging of Raynaud’s phenomenon, neurosensory manifestations, and vibration exposure in patients with hand-arm vibration injury
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Aug. 5, 2024
Abstract
Our
aim
was
to
explore
possible
relationships
between
serum
levels
of
biomarkers
in
patients
with
hand-arm
vibration
injury
relation
the
severity
vascular,
i.e.,
Raynaud’s
phenomenon
(RP),
and
neurosensory
manifestations,
current
exposure
level,
duration
exposure.
This
study
case
series
design
involved
92
diagnosed
injury.
Jonckheere’s
trend
test
used
assess
any
association
RP
as
well
graded
by
International
Consensus
Criteria.
Generalized
linear
models
adjustment
for
confounders
were
also
associations
and;
(1)
recorded
extent
finger
blanching
calculated
Griffin
score,
(2)
perception
thresholds,
(3)
magnitude
[A(8);
(m/s
2
)]
value,
(4)
years.
Serum
thrombomodulin,
von
Willebrand
factor,
calcitonin
gene
related
peptide
(CGRP),
heat
shock
protein
27,
caspase-3
positively
associated
RP.
CGRP
component.
No
shown
these
.
For
Intercellular
adhesion
molecule
1
monocyte
chemoattractant
1,
no
found
neither
nor
Levels
endothelial
or
dysfunction,
inflammation,
vasodilation,
neuroprotection,
apoptosis
Language: Английский
Autophagy-targeting modulation to promote peripheral nerve regeneration
Neural Regeneration Research,
Journal Year:
2024,
Volume and Issue:
20(7), P. 1864 - 1882
Published: May 10, 2024
Nerve
regeneration
following
traumatic
peripheral
nerve
injuries
and
neuropathies
is
a
complex
process
modulated
by
diverse
factors
intricate
molecular
mechanisms.
Past
studies
have
focused
on
that
stimulate
axonal
outgrowth
myelin
regeneration.
However,
recent
highlighted
the
pivotal
role
of
autophagy
in
regeneration,
particularly
context
injuries.
Consequently,
autophagy-targeting
modulation
has
emerged
as
promising
therapeutic
approach
to
enhancing
Our
current
understanding
suggests
activating
facilitates
rapid
clearance
damaged
axons
sheaths,
thereby
neuronal
survival
mitigating
injury-induced
oxidative
stress
inflammation.
These
actions
collectively
contribute
creating
favorable
microenvironment
for
structural
functional
A
range
autophagy-inducing
drugs
interventions
demonstrated
beneficial
effects
alleviating
neuropathy
promoting
preclinical
models
This
review
delves
into
regulation
cell
types
involved
summarizing
potential
can
be
harnessed
promote
this
process.
We
hope
our
will
offer
novel
insights
perspectives
exploitation
pathways
treatment
neuropathies.
Language: Английский
Elucidation of the role of microglia‐macrophage‐based neuroinflammation in neurological diseases
Neurology and Clinical Neuroscience,
Journal Year:
2024,
Volume and Issue:
12(6), P. 329 - 339
Published: May 22, 2024
Abstract
Objective
To
elucidate
the
roles
of
microglia
and
macrophages
in
neuroinflammation
their
dual
impact
on
progression
neurological
diseases.
Background
Microglia
are
integral
components
central
peripheral
nervous
systems,
where
they
play
important
maintaining
homeostasis,
influencing
disease
progression,
facilitating
repair
mechanisms.
Methods
The
review
integrates
recent
advancements
single‐cell
RNA
sequencing
that
highlight
extensive
heterogeneity
macrophages,
which
helps
understanding
wide‐ranging
different
conditions.
Results
analysis
reveals
have
a
nature,
capable
both
exacerbating
mitigating
processes
across
various
conditions,
including
Alzheimer’s
disease,
multiple
sclerosis,
stroke,
nerve
disorders,
brain
tumors.
Conclusion
Modulating
activity
offers
promising
avenue
for
therapeutic
interventions
disorders.
There
is
critical
need
further
research
to
fully
leverage
potential
targeting
enhance
patient
outcomes.
Language: Английский
PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats
Regenerative Medicine,
Journal Year:
2024,
Volume and Issue:
19(6), P. 327 - 343
Published: June 2, 2024
Background:
Adjunctive
pharmacological
treatment
may
improve
nerve
regeneration.
We
investigated
regeneration
processes
of
PXL01
–
a
lactoferrin-derived
peptide
after
repair
the
sciatic
in
healthy
Wistar
rats.
Materials
&
methods:
PXL01,
sodium
hyaluronate
(carrier)
or
chloride
was
administered
around
repair.
After
6
days
axonal
outgrowth,
Schwann
cell
response,
pan-
(CD68)
and
pro-healing
(CD206)
macrophages
nerve,
sensory
neuronal
response
dorsal
root
ganglia
(DRG)
expression
heat
shock
protein
27
(HSP27)
nerves
DRGs
were
analyzed.
Results:
Despite
lower
number
pan-macrophages,
other
variables
did
not
differ
between
groups.
Conclusion:
PLX01
applied
locally
inhibits
inflammation
through
pan-macrophages
repaired
without
any
impact
on
macrophages.
Language: Английский
Quantitative mass spectrometry analysis of the injured proximal and distal human digital nerve ends
Frontiers in Molecular Neuroscience,
Journal Year:
2024,
Volume and Issue:
17
Published: July 2, 2024
Introduction
Proteomic
analysis
of
injured
human
peripheral
nerves,
particularly
focusing
on
events
occurring
in
the
proximal
and
distal
nerve
ends,
remains
relatively
underexplored.
This
study
aimed
to
investigate
molecular
patterns
underlying
a
digital
injury,
differences
protein
expression
between
ends.
Methods
A
total
26
samples
(24
men;
2
women;
median
age
47
[30–66]
years),
harvested
during
primary
repair
within
48
h
post-injury
from
were
analyzed
using
mass
spectrometry.
Results
3,914
proteins
identified,
with
127
showing
significant
abundance
The
downregulation
end
was
associated
synaptic
transmission,
autophagy,
neurotransmitter
regulation,
cell
adhesion
migration.
Conversely,
upregulated
implicated
cellular
stress
response,
neuromuscular
junction
stability
muscle
contraction,
neuronal
excitability
release,
vesicle
recycling
axon
guidance
angiogenesis.
Discussion
Investigation
proteins,
functional
annotations
analysis,
ends
revealed
dynamic
responses
at
promoting
tissue
degeneration
restoration,
while
suppressing
non-essential
processes.
Language: Английский