SOS1 gene family in mangrove (Kandelia obovata): Genome-wide identification, characterization, and expression analyses under salt and copper stress

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 27, 2024

Abstract Background Salt Overly Sensitive 1 ( SOS1 ), a plasma membrane Na + /H exchanger, is essential for plant salt tolerance. damage significant abiotic stress that impacts species globally. All living organisms require copper (Cu), necessary micronutrient and protein cofactor many biological physiological processes. High Cu concentrations, however, may result in pollution inhibits the growth development of plants. The function production mangrove ecosystems are significantly impacted by rising salinity contamination. Results A genome-wide analysis bioinformatics techniques were used this study to identify 20 genes genome Kandelia obovata . Most found on dispersed over 11 18 chromosomes. Based phylogenetic analysis, KoSOS1s can be categorized into four groups, similar Solanum tuberosum obovata's gene family expanded due tandem segmental duplication. These homologs shared structures, according results conserved motif analysis. coding regions KoSOS1 consist amino acids ranging from 466 1221, while exons include 3 23. In addition, we 2.0 kb upstream promoter region contains several cis-elements associated with phytohormones responses. According expression experiments, seven randomly chosen experienced up- down-regulation their levels response (CuCl 2 ) stressors. Conclusions For first time, work systematically identified Our investigations also encompassed physicochemical properties, evolution, patterns, thereby furnishing theoretical framework subsequent research endeavours aimed at functionally characterizing throughout life cycle

Language: Английский

Genome-wide analysis and expression profiling of the polyamine oxidase gene family in Solanum tuberosum L.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Dec. 2, 2024

Polyamine oxidase (PAO) is a crucial enzyme involved in the breakdown of polyamines (PAs) plants. It not only regulates levels PAs, but also plays role oxidative decomposition PAs and release stress-related signals, contributing to plant's response resistance various adversities. While there have been numerous studies on PAO stress other crops, lack research this topic potatoes, major food crop. In study, we aimed explore biological function StPAO gene potato growth development, as well its expression patterns under stress. Using bioinformatics methods, identified 14 genes genome. Protein sequence comparisons revealed high similarity between proteins Arabidopsis. Chromosomal mapping structure analysis showed that were evenly distributed chromosome all contained an amino-oxidase domain. Furthermore, promoters these presence abiotic cis-acting elements, indicating their potential responding different stresses. To investigate stress, used qRT-PCR study temperature, drought, ABA Our results StPAO6 StPAO10 significantly up-regulated temperature they process temperatures. Similarly, StPAO1, StPAO3, StPAO4 drought potatoes' responses drought. After treatment, StPAO4, StPAO5, StPAO7, StPAO14 up-regulated, suggesting involvement chemical defense mechanisms. Interestingly, StPAO11–13 was inhibited by three conclusion, our highlights multifunctional nature family which coping with This deepens understanding provides reference for future function. serves theoretical basis breeding stress-resistant varieties future.

Language: Английский