Dimethyl phthalate exposure induces cognitive impairment through COX2-mediated microglial activation DOI Creative Commons
Genghuan Wang, Jian Shen,

Yingcong Lin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Abstract AIM We investigated the mechanism of action whereby exposure to dimethyl phthalate (DMP), an environmental pollutant, causes cognitive impairment. METHODS Network pharmacology was used analyze targets and regulatory networks associated with DMP-brain injury-cognitive The intake DMP simulated by exposure, Morris water maze adopted examine tissue levels inflammatory cytokines were detected. Brain damage assayed H&E Nissl staining, protein expression identified Western blotting. effects observed after separate application siRNA-COX2 celecoxib-COX2 inhibitors. In in vitro experiment, on microglia activation investigated. RESULTS revealed that PTGS2 (COX2) significantly correlated DMP, which could act through COX2. After mice showed significant brain injury impairment, along upregulation cytokines. inhibit ameliorate impairment mice. results activate cause neuroinflammation. CONCLUSION can induce neuroinflammation COX2-mediated microglia, resulting COX2 is important target for action.

Language: Английский

Astragalin improves cognitive disorder in Alzheimer's disease: Based on network pharmacology and molecular docking simulation DOI Creative Commons
Rui Du, Hongyan Pei, Zhongmei He

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(8)

Published: Aug. 1, 2024

We investigate the mechanism of action astragalin (AST) in treatment Alzheimer's disease (AD). Network pharmacology was conducted to analyze relationships among AST, AD, and neuroinflammation, The APP/PS1 transgenic mice with AD were used experiments; be specific, influence AST on behavior analyzed by Morris water maze eight-arm radial tests, tissue inflammatory factor levels detected ELISA, pathological changes H&E immunohistochemical staining. Analysis results network suggested that exerted multi-target effect neuroinflammation AD. Through molecular docking dynamics analyses, COX2 might target AST. Moreover, animal experimental demonstrated improved mice, enhanced motor memory abilities, meanwhile, it suppressed expression factors tissues activation microglial cells. this study discovers can suppress cell via improve

Language: Английский

Citations

5
A Review of Advances in Molecular Imaging of Rheumatoid Arthritis: From In Vitro to Clinic Applications Using Radiolabeled Targeting Vectors with Technetium-99m DOI Creative Commons
Muhammad Ali, Viviana Benfante, Domenico Di Raimondo

et al.

Life, Journal Year: 2024, Volume and Issue: 14(6), P. 751 - 751

Published: June 12, 2024

Rheumatoid arthritis (RA) is a systemic autoimmune disorder caused by inflammation of cartilaginous diarthrodial joints that destroys and cartilage, resulting in synovitis pannus formation. Timely detection effective management RA are pivotal for mitigating inflammatory consequences, potentially influencing disease progression. Nuclear medicine using radiolabeled targeted vectors presents promising avenue diagnosis response to treatment assessment. Radiopharmaceutical such as technetium-99m (

Language: Английский

Citations

4
Effects of Cigarette Smoking and 3‐Day Smoking Abstinence on Translocator Protein 18 kDa Availability: A [18F]FEPPA Positron Emission Tomography Study DOI Creative Commons
Arthur L. Brody,

Andre Y. Sanavi,

Renee Beverly‐Aylwin

et al.

Addiction Biology, Journal Year: 2025, Volume and Issue: 30(4)

Published: April 1, 2025

ABSTRACT With the many negative health consequences of cigarette smoking, quitting is known to improve in multiple domains. Using positron emission tomography/computed tomography (PET/CT) scanning, our group previously demonstrated that smokers have lower levels than nonsmokers translocator protein binding both acutely and following overnight abstinence. Here, we sought determine effects longer smoking abstinence on this marker gliosis for microglia astroglia, as well explore associations between smoking‐related symptoms. This observational study was performed an academic VA medical centre. Fifty‐nine generally healthy Veterans who were either ( n = 15) or 44) participated study. Participants completed intake visit evaluate inclusion/exclusion criteria, [ 18 F]FEPPA PET/CT scanning a structural magnetic resonance imaging scan. Smokers alternately assigned smoke satiety 24) before undergo three nights continuous prior using contingency management 20 protocol scanning). The smoker had significantly mean whole brain (WB) standardized uptake value (SUV) nonsmoking (−15.3%) abstinent (−12.3%) groups. control groups WB SUVs not different from one another (3.0% difference). In exploratory analysis, significant inverse relationship found mood ratings smokers, indicating higher TSPO associated with worse mood. central findings here support previous studies demonstrating satiated imply normalization elimination constituents body, although other explanations results (e.g., alterations radioligand delivery clearance by constituents) are possible. These may represent unknown benefit smoking.

Language: Английский

Citations

0
Assessment of basal TSPO expression and [18F]DPA-714 biodistribution in healthy mice and post-ischemic brain using PET imaging DOI
Brígida Gomes de Almeida Schirmer, Juliana de Oliveira Silva,

D. Navarro

et al.

Brazilian Journal of Radiation Sciences, Journal Year: 2025, Volume and Issue: 12(4A (Suppl.)), P. e2812 - e2812

Published: April 28, 2025

Positron emission tomography (PET) is an important tool in preclinical studies small animals, providing real-time insights into biochemical, metabolic, physiological, and functional processes. PET imaging also enables the assessment of biological responses biodistribution novel radiolabeled compounds within a single animal, minimizing need for larger animal groups. In particular, with [18F]DPA-714, Translocator Protein (TSPO) ligand, has shown high predictive prognostic value diseases associated neuroinflammation correlates well outcomes. this study, basal expression TSPO was investigated vivo C57BL/6 mice proposed as method to track new molecules. Male aged 6–9 weeks weighing 20–30 g were divided healthy ischemic The group subjected transient global cerebral ischemia induced by 25 min bilateral common carotid artery occlusion (BCCAO) followed reperfusion. Baseline [18F]DPA-714 performed static whole-body scans at 0-20, 20-40, 40-60, 60-80 post-injection intervals. After ischemia, used examine uptake [18F]DPA-714. results confirm that effective, non-invasive technique studies. Analysis SUVmean, SUVmax, SUVpeak metrics showed increased sensitivity brain following highlighting its importance models. Furthermore, baseline observed multiple organs, reflecting metabolic clearance pathways. comparable muscle underscores potential reliable marker studying TSPO-related inflammatory

Language: Английский

Citations

0
Dimethyl phthalate exposure induces cognitive impairment via COX2-mediated neuroinflammation DOI Creative Commons
Haoyuan Yin, Jian Shen,

Xiaoying Qian

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 284, P. 117039 - 117039

Published: Sept. 12, 2024

Language: Английский

Citations

2
Dimethyl phthalate exposure induces cognitive impairment through COX2-mediated microglial activation DOI Creative Commons
Genghuan Wang, Jian Shen,

Yingcong Lin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Abstract AIM We investigated the mechanism of action whereby exposure to dimethyl phthalate (DMP), an environmental pollutant, causes cognitive impairment. METHODS Network pharmacology was used analyze targets and regulatory networks associated with DMP-brain injury-cognitive The intake DMP simulated by exposure, Morris water maze adopted examine tissue levels inflammatory cytokines were detected. Brain damage assayed H&E Nissl staining, protein expression identified Western blotting. effects observed after separate application siRNA-COX2 celecoxib-COX2 inhibitors. In in vitro experiment, on microglia activation investigated. RESULTS revealed that PTGS2 (COX2) significantly correlated DMP, which could act through COX2. After mice showed significant brain injury impairment, along upregulation cytokines. inhibit ameliorate impairment mice. results activate cause neuroinflammation. CONCLUSION can induce neuroinflammation COX2-mediated microglia, resulting COX2 is important target for action.

Language: Английский

Citations

1