Isoeugenol Inhibits Adipogenesis in 3T3-L1 Preadipocytes with Impaired Mitotic Clonal Expansion

Nutrients, Journal Year: 2024, Volume and Issue: 16(9), P. 1262 - 1262

Published: April 24, 2024

Isoeugenol (IEG), a natural component of clove oil, possesses antioxidant, anti-inflammatory, and antibacterial properties. However, the effects IEG on adipogenesis have not yet been elucidated. Here, we showed that blocks in 3T3-L1 cells at an early stage. inhibits lipid accumulation adipocytes concentration-dependent manner reduces expression mature adipocyte-related factors including PPARγ, C/EBPα, FABP4. treatment different stages inhibited adipocyte differentiation by suppressing stage, as confirmed biomarkers. The stage stimulates growth-arrested preadipocytes to enter mitotic clonal expansion (MCE) initiates their into regulating cell cycle-related factors. arrested G0/G1 phase cycle attenuated cyclinD1, CDK6, CDK2, cyclinB1 during MCE Furthermore, suppresses reactive oxygen species (ROS) production ROS-related antioxidant enzymes, superoxide dismutase1 (SOD1) catalase. proliferation-related biomarkers, pAKT pERK1/2, was preadipocytes. These findings suggest it is potential therapeutic agent for obesity.

Language: Английский

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Targeting lipid droplets and lipid droplet-associated proteins: a new perspective on natural compounds against metabolic diseases

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Sept. 4, 2024

Language: Английский

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14-3-3ζ regulates adipogenesis by modulating chromatin accessibility during the early stages of adipocyte differentiation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 19, 2024

ABSTRACT We previously established the scaffold protein 14-3-3ζ as a critical regulator of adipogenesis and adiposity, but temporal specificity its action during adipocyte differentiation remains unclear. To decipher if exerts regulatory functions on mature adipocytes or adipose precursor cells (APCs), we generated Adipoq 14-3-3ζKO Pdgfra mouse models. Our findings revealed pivotal role for in APC sex-dependent manner, whereby male female mice display impaired potentiated weight gain, respectively, well fat mass. better understand how regulates adipogenic transcriptional program APCs, CRISPR-Cas9 was used to generate TAP-tagged 14-3-3ζ-expressing 3T3-L1 preadipocytes. Using these cells, examined nuclear interactome is enriched with regulators differentiation. Regulators chromatin remodeling, such DNMT1 HDAC1, were 14-3-3ζ, their activities impacted upon depletion. The interactions between chromatin-modifying enzymes suggested that may control remodeling adipogenesis, this confirmed by ATAC-seq, which depletion accessibility up 1,244 regions corresponding part genes, promoters, enhancers initial stages adipogenesis. Moreover, 14-3-3ζ-dependent found directly correlate expression key genes. Altogether, our study establishes crucial epigenetic highlights usefulness deciphering identify novel pro-adipogenic factors pathways.

Language: Английский

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Inhibition of Differentiation of 3T3-L1 Cells by Increasing Glioma-Associated Oncogene Expression in Chrysanthemum indicum L. Using Lactococcus lactis KCTC 3115

Preventive Nutrition and Food Science, Journal Year: 2024, Volume and Issue: 29(4), P. 533 - 545

Published: Dec. 30, 2024

The inhibitory effect of

Language: Английский

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