Construction of a miRNA Panel for Differentiating Lung Adenocarcinoma Brain Metastases and Glioblastoma

Cancers, Journal Year: 2025, Volume and Issue: 17(4), P. 581 - 581

Published: Feb. 8, 2025

Brain metastases (BM) are the most common type of intracranial malignant tumor and associated with high mortality. More than 50% BM cases originate from lung cancer, adenocarcinoma (LUAD) is commonly development (25%). The differential diagnosis solitary glioblastoma (GBM)-one aggressive fatal brain tumors-remains a considerable challenge. Given major role microRNAs (miRNAs) in regulating gene expression, their clinical potential as biomarkers for prognosis offers significant promise. Next-generation RNA Sequencing (RNA-seq) was used to assess miRNA expression profiles 6 LUAD-BM, GBM, control (non-tumoral tissue samples) human samples. miRNAs exhibiting LUAD-BM patients comparison both subjects GBM were selected validation through RT-qPCR. analysis RNA-seq data revealed presence 229 differentially expressed between samples 46 LU-AD-BM Eight further analysis, four which upregulated downregulated, based on differences levels observed other two groups, confirmed Mann-Whitney U test. Functional enrichment also conducted miRNA-centered target performed using miRNet tool. To diagnostic these miRNAs, we receiver operating characteristic (ROC) curve analysis. A panel eight identified samples, accuracy distinguishing

Language: Английский

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The PACT Network: PRL, ARL, CNNM, and TRPM Proteins in Magnesium Transport and Disease

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1528 - 1528

Published: Feb. 12, 2025

Magnesium, the most abundant divalent metal within cell, is essential for physiological function and critical in cellular signaling. To maintain homeostasis, intracellular magnesium levels are tightly regulated, as dysregulation linked to numerous diseases, including cancer, diabetes, cardiovascular disorders, neurological conditions. Over past two decades, extensive research on magnesium-regulating proteins has provided valuable insight into their pathogenic therapeutic potential. This review explores an emerging mechanism of homeostasis involving PRL (phosphatase regenerating liver), ARL (ADP ribosylation factor-like GTPase family), CNNM (cyclin cystathionine β-synthase domain transport mediator), TRPM (transient receptor potential melastatin) families, collectively termed herein PACT network. While each protein been studied its individual signaling disease contexts, interactions suggest a broader regulatory network with consolidates current knowledge proteins' structure, function, identifies gaps encourage future investigation. As field continues advance, understanding offers new opportunities basic development targeting magnesium-related disorders.

Language: Английский

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Crosstalk between Calcium and Reactive Oxygen Species Signaling in Cancer Revisited

Cell Calcium, Journal Year: 2025, Volume and Issue: unknown, P. 103014 - 103014

Published: March 1, 2025

Language: Английский

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Targeting eukaryotic elongation factor 2 kinase (eEF2K) with small-molecule inhibitors for cancer therapy

Drug Discovery Today, Journal Year: 2024, Volume and Issue: 29(10), P. 104155 - 104155

Published: Aug. 28, 2024

Language: Английский

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S‐acylation of Ca²⁺ transport proteins in cancer

Chronic Diseases and Translational Medicine, Journal Year: 2024, Volume and Issue: 10(4), P. 263 - 280

Published: Aug. 14, 2024

Abstract Alterations in cellular calcium (Ca 2+ ) signals have been causally associated with the development and progression of human cancers. Cellular Ca are generated by channels, pumps, exchangers that move ions across membranes decoded effector proteins cytosol or organelles. S‐acylation, reversible addition 16‐carbon fatty acids to proteins, modulates activity transporters altering their affinity for lipids, enzymes mediating this post‐translational modification also linked several types Here, we compile studies reporting an association between S‐acylation specific cancers, as well predicting transporters. We then discuss potential role oncogenic a subset transport involved cancer.

Language: Английский

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Expression Profiling Identified TRPM7 and HER2 as Potential Targets for the Combined Treatment of Cancer Cells

Cells, Journal Year: 2024, Volume and Issue: 13(21), P. 1801 - 1801

Published: Oct. 31, 2024

TRPM7 is a divalent cation-permeable channel that highly active in cancer cells. The pharmacological inhibitors of have been shown to suppress the proliferation tumor cells, highlighting as new anticancer drug target. However, potential benefit combining with conventional therapies remains unexplored. Here, we used genome-wide transcriptome profiling human leukemia HAP1 cells examine cellular responses caused by application NS8593, potent inhibitor channel, comparison two independent knockout mutations

Language: Английский

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Calcium channels as therapeutic targets in head and neck squamous cell carcinoma: current evidence and clinical trials

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Dec. 20, 2024

Head and neck squamous cell carcinoma (HNSCC) originates from the mucosal epithelium of oral cavity, pharynx, larynx, is marked by high rates recurrence metastasis. Calcium signaling associated with progression HNSCC development drug resistance. Changes in calcium ion flow can trigger severe pathophysiological processes, including malignant transformation, tumor proliferation, epithelial-mesenchymal transition, apoptosis evasion. channels regulate facilitate these processes. Remodeling has become one most prevalent adaptive mechanisms cancer cells. Preclinical clinical evidence indicates that alterations are crucial for HNSCC. This review examines role evaluates current trials targeting to assess feasibility signaling-based therapies

Language: Английский

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