Genome-wide identification and comparative analysis of the AP2/ERF gene family in Prunus dulcis and Prunus tenella: expression of PdAP2/ERF genes under freezing stress during dormancy

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 31, 2025

The AP2/ERF (APETALA2/ethylene responsive factor) transcription factor family, one of the largest in plants, plays a crucial role regulating various biological processes, including plant growth and development, hormone signaling, stress response. This study identified 114 116 genes genomes 'Wanfeng' almond (Prunus dulcis) 'Yumin' wild dwarf tenella), respectively. These were categorized into five subfamilies: AP2, DREB, ERF, RAV, Soloist. PdAP2/ERF PtAP2/ERF members both demonstrated high conservation protein motifs gene structures. Members families unevenly distributed across eight chromosomes, with 30 27 pairs segmental duplications 15 18 tandem repeated genes, promoter regions family contained numerous important cis-elements related to regulation, Expression pattern analysis revealed that exhibited characteristics under freezing at different temperatures perennial dormant branches. Quantitative fluorescence indicated might be more intensely expressed phloem branches almond, opposite trend observed xylem. compared initially explored expression patterns xylem findings provide theoretical foundation for future research on improvement breeding, as well molecular mechanisms underlying resistance stress.

Language: Английский

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Exploring differentially expressed genes and associated biological processes across embryos with different seed dormancy in barley (Hordeum vulgare)

Crop Science, Journal Year: 2025, Volume and Issue: 65(1)

Published: Jan. 1, 2025

Abstract Barley ( Hordeum vulgare ) has a wide range of seed dormancy. Seed dormancy is crucial trait for plant survival and crop production. To explore genes functions associated with the difference in barley dormancy, we performed differentially expressed gene ontology (GO) enrichment analyses on embryos from wild accession H602 strong cultivated malting Kanto Nakate Gold (KNG) weak reciprocal crosses between two × KNG H602. RNA was extracted seeds harvested at physiological maturity dried 1 week. We also assessed expression levels known quantitative locus linked to In KNG, 417 showed significantly higher than H602, 14 GO terms enriched, including stress response sugar metabolism. Genes involved DNA repair were enriched highly KNG. 198 60 development auxin transport. translation Our findings indicated that abiotic response, metabolism, would be whereas barley.

Language: Английский

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Chromosome-Level Genome Assembly of Myricaria laxiflora and Identification of Candidate MylAP2/ERF Genes Involved in Flooding Stress

Forests, Journal Year: 2025, Volume and Issue: 16(2), P. 295 - 295

Published: Feb. 8, 2025

M. laxiflora is an endangered plant that grows in the Yangtze River floodplain of China and often suffers from flooding stress. Due to lack a reference genome for laxiflora, molecular regulatory mechanism waterlogging stress this remains unclear. In study, we report high-quality with size 1.29 Gb. A total 23,666 gene-encoding proteins 5457 ncRNAs were predicted genome. comparative analysis revealed 902 4299 gene families significantly expanded contracted, respectively, laxiflora. The expansions related “response stress”, abiotic stimulus”, oxygen-containing compounds” pathways. genome, 101 MylAP2/ERF genes identified divided into five subgroups. Several MeJA-, ABA-, hypoxia-responsive elements found promoter regions these genes. According transcriptome data analysis, 74 responded Moreover, three (MylAP2/ERF49/78/91) belong same branch as RAP2.2 exhibited different expression trends under Our results provide valuable information on

Language: Английский

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Genes determining drought resistance of common wheat (Triticum aestivum L.)

Visnik ukrains kogo tovaristva genetikiv i selekcioneriv, Journal Year: 2025, Volume and Issue: 22(1-2), P. 67 - 80

Published: Jan. 1, 2025

The aim of the work is to elucidate molecular mechanisms plant resistance abiotic stress and role specific genes involved in determining drought plants, particularly bread wheat. plants biotic environmental factors associated with activation a complex phosphorylation/dephosphorylation cascade mediated by protein kinases phosphatases. result this signaling activation/repression transcription that are able regulate expression certain directly related adaptation stress. Transcription can be classified into 60 families based on similarities primary and/or three-dimensional structure DNA binding domains, oligomerization patterns, post-translational modifications, but most studied date 8: AP2/ERF, MYB, bHLH, NAC, WRKY, bZIP, HSF HDZip. This paper discusses peculiarities responses, wheat, caused drought. It separately regulation factor activity during stress, within framework response osmotic which shaped multiple pathways. combination these pathways ensures temporal spatial patterns gene expression, play significant immediate wheat water deficit.

Language: Английский

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Compost and biochar application mitigates salt stress on barley: monitoring of agronomic, physiological, biochemical and molecular parameters

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

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A novel trait to reduce the mechanical damage of peach fruits at harvest: The first genetic dissection study for peduncle length

Molecular Breeding, Journal Year: 2025, Volume and Issue: 45(3)

Published: Feb. 24, 2025

Abstract In peach, a long peduncle can help minimize mechanical damages/physical injuries in the fruit at harvest and also be useful postharvest handling transportation. view of genetically dissecting length (PL) we have performed Quantitative Trait Locus (QTL) mapping study for PL using F 2 progeny 117 individuals from cross ‘PI 91459 [NJ Weeping]’ x ‘Bounty’ (WxBy). The was phenotyped three years (2011, 2012 2014) QTL analysis four methods: Kruskall-Wallis, Interval Mapping, Multiple Mapping Genome-Wide Composite Mapping. led to identification 9 QTLs distributed on linkage groups (LG) 1, 2, 4, 5, 6 7. A stable identified LG6 (22,978,897 24,666,094 bp) explained up 63% phenotypic variance. Within genetic interval potential candidate genes with functional annotation encompassing cellular expansion, hormone regulation, transcriptional developmental processes such as meristem development, responses environmental cues were found. results reported this represent first insight into basis step forward towards introduction novel traits peach commercial breeding order problems related damage/injuries fruits that commonly might occur during post-harvest processes.

Language: Английский

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Evolutionary and functional analysis of the DIR gene family in Moso bamboo: insights into rapid shoot growth and stress responses

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 25, 2025

Dirigent (DIR) proteins are key regulators of lignin and lignan biosynthesis play critical roles in plant hormone responses, abiotic stress tolerance, growth development. This study identified characterized 47 PeDIR genes Moso bamboo, classifying them into three groups. Phylogenetic comparative analyses revealed strong evolutionary conservation, with the bamboo being most closely related to those rice maize. DIR within each subfamily exhibited high conservation motif composition, domain structure, 3D configuration. Subcellular localization protein interaction studies further elucidated gene functions. Specifically, PeDIR02 primarily localized cell membrane was shown be unable form homodimers yeast two-hybrid (Y2H) assays. Transcriptome expression involvement rapid shoot growth, indicating wall modification. qRT-PCR data also demonstrated responsiveness these hormones stresses, such as drought salinity. constructed first comprehensive regulatory network between transcription factors (TFs) genes, identifying ERF, DOF, MYB TFs synergistic expression.

Language: Английский

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Overexpression of a Fragaria × ananassa AP2/ERF Transcription Factor Gene (FaTINY2) Increases Cold and Salt Tolerance in Arabidopsis thaliana

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2109 - 2109

Published: Feb. 27, 2025

The AP2/ERF family of transcription factors is one the most conserved and important factor families, it ubiquitous in plants. It plays an essential role plant morphogenesis, molecular mechanisms stress responses, hormone signaling pathways, synthesis secondary metabolites. FaTINY2 was cloned from octaploid strawberry Fragaria × ananassa for this investigation. Bioinformatics revealed that protein possesses a AP2 domain localized nucleus. When expressed plants, quantitative analysis gene tissue-specific. There are lower contents reactive oxygen species (ROS) malondialdehyde (MDA), higher proline, chlorophyll, activities catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) transgenic Arabidopsis thaliana than wild type (WT) unload line (UL) plants under cold salt stress. enhancing tolerance by regulating few genes linked to response. findings study were more tolerant WT UL In addition offering theoretical reference production stress, research established groundwork exploration into which strawberries respond high

Language: Английский

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An ERF Gene DcERF3 of Dendrobium catenatum Improves Salt Tolerance in Arabidopsis

Molecular Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Language: Английский

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OsERF7 Negatively Regulates Resistance to Sheath Blight Disease by Inhibiting Phytoalexin Biosynthesis

Rice Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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