Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration DOI
Wei-Cheng Chen, Dachuan Liu, Kai Lü

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Musculoskeletal diseases have emerged as the leading cause of disability worldwide, with their prevalence increasing annually. In light this escalating health challenge, organoids, an emerging technology in tissue engineering, offer promising solutions for disease modeling, drug screening, regeneration, and repair processes. The successful development musculoskeletal organoids represents a significant breakthrough, providing novel platform studying facilitating discovery new treatments. Moreover, serve valuable complements to traditional 2D culture methods animal models, offering rich insights into biology. This review provides overview organoid technology, outlining construction processes various highlighting similarities differences. Furthermore, challenges associated systems are discussed future perspectives offered.

Language: Английский

Clinical applications of human organoids DOI
Monique M.A. Verstegen, Robert P. Coppes, Anne Béghin

et al.

Nature Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Language: Английский

Citations

9
Targeting TDP-43 Proteinopathy in hiPSC-Derived Mutated hNPCs with Mitoxantrone Drugs and miRNAs DOI Creative Commons
Uzair Ahmad Ansari, Ankita Srivastava, Ankur Kumar Srivastava

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 410 - 410

Published: March 25, 2025

Background/Objectives: TDP-43 mutation-driven Amyotrophic Lateral Sclerosis (ALS) motor neuron disease is one of the most prominent forms (approximately 97%) in cases sporadic ALS. Dysfunctional autophagy and lysosomal function are prime mechanisms behind Mitoxantrone (Mito), a synthetic doxorubicin analog, an inhibitor DNA RNA synthesis/repair via intercalating with nitrogenous bases inhibiting topoisomerase II. The therapeutic potential miRNAs associated conditions has also been reported. This study explores Mito along against mutated protein-induced proteinopathy human-induced pluripotent stem cell (hiPSC)-derived human neural progenitor cells (hNPCs). Methods: HiPSCs for were differentiated into hNPCs used to explore at concentration 1 μM 24 h (the identified non-cytotoxic dose). effects on miRNA expression various cellular parameters such as mitochondrial dynamics, autophagy, stress granules assessed using high-throughput Open Array technique, immunocytochemistry, flow cytometry, immunoblotting, bioenergetic assay. Results: Mutated protein accumulation causes granule formation (G3BP1), dysfunction, SOD1 accumulation, hyperactivated ER hNPCs. show dysregulation six (miR-543, miR-34a, miR-200c, miR-22, miR-29b, miR-29c) A significant restoration mutation-induced alterations could be witnessed upon exposure Mito. Conclusions: Our indicates that miR-543, miR-34a have antisense alone combination Mitoxantrone.

Language: Английский

Citations

0
Intelligent Manufacturing for Osteoarthritis Organoids DOI Creative Commons
Xin Lyu, Jian Wang, Jiacan Su

et al.

Cell Proliferation, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

ABSTRACT Osteoarthritis (OA) is the most prevalent degenerative joint disease worldwide, imposing a substantial global burden. However, its pathogenesis remains incompletely understood, and effective treatment strategies are still lacking. Organoid technology, in which stem cells or progenitor self‐organise into miniature tissue structures under three‐dimensional (3D) culture conditions, provides promising vitro platform for simulating pathological microenvironment of OA. This approach can be employed to investigate mechanisms, carry out high‐throughput drug screening facilitate personalised therapies. review summarises structure, OA manifestations, thereby establishing context application organoid technology. It then examines components arthrosis system, specifically addressing cartilage, subchondral bone, synovium, skeletal muscle ligament organoids. Furthermore, it details various constructing organoids, including considerations cell selection, classification fabrication techniques. Notably, this introduces concept intelligent manufacturing organoids by incorporating emerging engineering technologies such as artificial intelligence (AI) process, forming an innovative software hardware cluster. Lastly, discusses challenges currently facing highlights future directions rapidly evolving field. By offering comprehensive overview state‐of‐the‐art methodologies challenges, anticipates that intelligent, automated will expedite fundamental research, discovery translational applications orthopaedic

Language: Английский

Citations

0
Exploring the Role of Circular RNA in Bone Biology: A Comprehensive Review DOI Creative Commons
Maria Teresa Valenti,

Roberta Zerlotin,

Mattia Cominacini

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(12), P. 999 - 999

Published: June 7, 2024

Circular RNAs (circRNAs) have emerged as pivotal regulators of gene expression with diverse roles in various biological processes. In recent years, research into circRNAs’ involvement bone biology has gained significant attention, unveiling their potential novel and biomarkers bone-related disorders diseases. CircRNAs, characterized by closed-loop structure, exhibit stability resistance to degradation, underscoring functional significance. tissue, circRNAs are involved critical processes such osteogenic differentiation, osteoclastogenesis, remodeling through intricate molecular mechanisms including microRNA regulation. Dysregulated associated disorders, suggesting diagnostic prognostic biomarkers. The therapeutic targeting these holds promise for addressing conditions, offering new perspectives precision medicine. Thus, constitute integral components regulatory networks, impacting both physiological homeostasis pathological conditions. This review provides a comprehensive overview biology, emphasizing mechanisms, implications, potential.

Language: Английский

Citations

2
Genomic Precision: Unveiling the Transformative Role of Genome Editing in Advancing Genomics Research and Applications DOI
Gholamreza Abdi,

Nil Patil,

Prachi Mishra

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

1
Molecular diagnostic approach to rare neurological diseases from a clinician viewpoint DOI Creative Commons

Jin Sook Lee

Genomics & Informatics, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 10, 2024

Advancements in sequencing technology have significantly enhanced diagnostic capabilities for rare neurological diseases. This progress molecular diagnostics can greatly impact clinical management and facilitate the development of personalized treatments patients with Neurologists expertise should raise awareness, as phenotyping remains crucial making a diagnosis, even genomics era. They prioritize different types genomic tests, considering both benefits limitations inherent to each test. Notably, long-read is being utilized cases suspected involve repeat expansion disorders or complex structural variants. Repeat are highly prevalent diseases, particularly within ataxia group. Significant efforts, including periodic reanalysis, data sharing, integration multi-omics studies, be directed toward that remain undiagnosed after standard next-generation sequencing.

Language: Английский

Citations

1
Aberrant evoked calcium signaling and nAChR cluster morphology in a SOD1 D90A hiPSC-derived neuromuscular model DOI Creative Commons

Nathalie Couturier,

Sarah Janice Hörner, Elina Nürnberg

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: June 20, 2024

Familial amyotrophic lateral sclerosis (ALS) is a progressive neuromuscular disorder that due to mutations in one of several target genes, including SOD1 . So far, clinical records, rodent studies, and vitro models have yielded arguments for either primary motor neuron disease, or pleiotropic pathogenesis ALS. While mouse lack the human origin, using induced pluripotent stem cells (hiPSC) been recently developed addressing ALS pathogenesis. In spite improvements regarding generation muscle from hiPSC, degree maturation resulting these protocols has remained limited. To fill shortcomings, we here present new protocol an enhanced myotube differentiation hiPSC with option further upon coculture hiPSC-derived neurons. The described model first yield combination key myogenic features are consistent sarcomeric organization association complex nAChR clusters myotubes derived control hiPSC. this model, carrying D90A mutation had reduced expression markers, sarcomeres, morphologically different clusters, altered nAChR-dependent Ca 2+ response compared myotubes. Notably, trophic support provided by neurons cluster differences between summary, novel yields evidence both muscle-intrinsic nerve-dependent aspects dysfunction -based

Language: Английский

Citations

0
MeHA: A Computational Framework in Revealing the Genetic Basis of Animal Mental Health Traits Under an Intensive Farming System—A Case Study in Pigs DOI Creative Commons

J. Z. Jiang,

Lili Xu,

Zhuang Yue

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(10), P. 843 - 843

Published: Oct. 21, 2024

Intensively farmed animals such as pigs inevitably experience a certain degree of psychological stress, which leads to reduction in production performance. Mental health traits are currently difficult measure, resulting gap understanding their genetic basis. To address this challenge, we propose computational framework called mental (MeHA), capable revealing genes related animal traits. Using MeHA, identified 109 candidate associated with pig and discovered intricate connections critical functions, memory, cognition, neural development, essential components cognitive Importantly, our findings provide evidence the potential impact these on economically important traits, including meat quality piglet survival. This research underscores importance studies enhancing behavior well promoting agricultural practices. By applying approach study basis case, confirmed that is an effective way reveal factors affecting contributes welfare has implications for human disorders.

Language: Английский

Citations

0
Brain Organoids and the Law DOI

A. Iyer

Published: Jan. 1, 2024

Language: Английский

Citations

0
Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration DOI
Wei-Cheng Chen, Dachuan Liu, Kai Lü

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Musculoskeletal diseases have emerged as the leading cause of disability worldwide, with their prevalence increasing annually. In light this escalating health challenge, organoids, an emerging technology in tissue engineering, offer promising solutions for disease modeling, drug screening, regeneration, and repair processes. The successful development musculoskeletal organoids represents a significant breakthrough, providing novel platform studying facilitating discovery new treatments. Moreover, serve valuable complements to traditional 2D culture methods animal models, offering rich insights into biology. This review provides overview organoid technology, outlining construction processes various highlighting similarities differences. Furthermore, challenges associated systems are discussed future perspectives offered.

Language: Английский

Citations

0