Genome-wide identification, characterization and expression of C2H2 zinc finger gene family in Opisthopappus species under salt stress

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: April 19, 2024

Abstract Background The C2H2 zinc finger protein family plays important roles in plants. However, precisely how C2H2s function Opisthopappus ( taihangensis and longilobus ) remains unclear. Results In this study, a total of 69 OpC2H2 genes were identified clustered into five Groups. Seven tandem ten fragment repeats found OpC2H2s, which underwent robust purifying selection. Of the motifs, motif 1 was present all OpC2H2s conserved at binding sites. Most possessed few introns exons that could rapidly activate react when faced with stress. promoter sequences mainly contained diverse regulatory elements, such as ARE, ABRE, LTR. Under salt stress, two up-regulated (OpC2H2-1 OpC2H2-14) one down-regulated gene (OpC2H2-7) might serve key transcription factors through ABA JA signaling pathways to regulate growth development species. Conclusion above results not only help understand but also drive progress genetic improvement for tolerance

Language: Английский

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MCTASmRNA: A deep learning framework for alternative splicing events classification

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139941 - 139941

Published: Jan. 1, 2025

Language: Английский

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Transcriptome analysis revealed alternative splicing landscape in response to gray-blight disease in tea plant

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120571 - 120571

Published: Jan. 21, 2025

Language: Английский

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Identification and expression responses of TCP gene family in Opisthopappus taihangensis under abiotic stress

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 6, 2025

The TCP gene family plays pivotal roles in the development and abiotic stress responses of plants; however, no data has been provided for this Opisthopappus taihangensis. Based on O. taihangensis genome, 14 genes were identified divided into two classes (I II). After tandem segmental duplication/whole-genome duplication (WGD), more loss less gain events OtTCPs occurred, which might be related with underwent purifying selection during evolution. conserved motifs structures OtTCP contained light response, growth development, hormone stress-related cis-acting elements. Different genes, even duplicated pairs, could expressed different tissues, implied that had diverse function. Among OtTCPs, OtTCP4, 9 11 CYC clade (Class II) presented a relative wide expression pattern or one intron. three regarded as important candidate factors growth, response. These results some clues references further in-depth exploration resistance mechanisms, well those other unique eco-environment plants.

Language: Английский

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Cliff-dwelling plants: rare and precious species in harsh habitats

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 5, 2025

Cliffs represent unique ecosystems that harbor diverse rare cliff-dwelling plants (CDPs), which play critical roles in enhancing biodiversity and maintaining ecological stability. Despite substantial advances CDP research enabled by modern biological technologies, particularly diversity assessment, taxonomic classification, conservation strategies, the field lacks comprehensive syntheses of recent progress, impeding efficient identification emerging directions. This review systematically examines five key domains: (1) The potential application value CDPs; (2) cliff habitat ecology, focusing on abiotic factors such as microclimate, edaphic properties, water conditions, well biotic components; (3) adaptive strategies CDPs, encompassing morphological specializations, physiological resilience mechanisms, early growth stage, reproductive characteristics, molecular-level evolutionary adaptations, genetic patterns; (4) technological advancements methodologies; (5) conducting a comparative analysis between situ protection measures ex preservation approaches. Our reveals three gaps demanding immediate attention: need for long-term monitoring habitats their microenvironmental dynamics, imperative to understand CDPs’ adaptations extreme environments, necessity elucidate molecular mechanisms governing plant-environment interactions within these ecosystems. Future should prioritize development innovative tools, advanced interdisciplinary methodologies fill existing knowledge field. Such efforts will not only complex adaptation CDPs but also provide scientific foundation effective ecosystem management.

Language: Английский

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The Regulatory Roles of RNA-Binding Proteins in Plant Salt Stress Response

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1402 - 1402

Published: May 7, 2025

Salt stress is one of the most prominent abiotic stresses. Behind intricate adaptive responses plants to salt stress, regulation gene expression assumes a pivotal role. Complementing transcriptional mechanisms, post-transcriptional performed by RNA-binding proteins provides an additional layer control through sophisticated molecular machinery. RBPs interact with both RNA molecules and protein partners coordinate metabolism and, thus, fine-tune salt-responsive genes, enabling rapidly adapt ionic challenges. This review systematically evaluates functional roles localized in distinct subcellular compartments, including nuclear, cytoplasmic, chloroplastic, mitochondrial systems, mediating regulatory networks under salinity Specific classes are discussed detail, glycine-rich (GR-RBPs), serine/arginine-rich splicing factors (SR proteins), zinc finger domain-containing proteins, DEAD-box helicases (DBRHs), KH Pumilio (PUMs), pentatricopeptide repeat (PPRs), involved cytoplasmic granule formation. By integrating their localization current mechanistic insights, this concludes summarizing knowledge highlighting potential future research directions, aiming inspire further investigations into complex network modulating plant facilitating development strategies enhance tolerance.

Language: Английский

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Potential Response Patterns of Endogenous Hormones in Cliff Species Opisthopappus taihangensis and Opisthopappus longilobus under Salt Stress

Plants, Journal Year: 2024, Volume and Issue: 13(4), P. 557 - 557

Published: Feb. 19, 2024

When plants are exposed to salt stress, endogenous hormones essential for their responses through biosynthesis and signal transduction pathways. However, the roles of in two cliff species (Opisthopappus taihangensis Opisthopappus longilobus genus)) Taihang Mountains under stress have not been investigated date. Following different time treatments 500 mM concentrations, 239 differentially expressed gene (DEG)-related were identified that exhibited four change trends, which Profile 47 upregulated both species. The C-DEG genes AUX, GA, JA, BR, ETH, ABA significantly enriched (O. taihangensis) longilobus). During responsive process, mainly JA triggered Subsequently, crosstalk further influenced EHT, ABA, MAPK pathways improve resistance Within protein–protein interactions (PPI), seven proteins highest interactions, primarily involved downregulated (SAUR GA3ox) eight (ACX, MFP2, JAZ, BRI1, BAK1, ETR, EIN2, SNRK2) above more expression ZEP (in pathway), DELLA GA signaling ABF ERF1 ETH pathway) O. revealed it had a relatively higher than longilobus. This patterns between similarities differences. results this investigation shed light on potential adaptive mechanisms environments, while laying foundation study other Mountains.

Language: Английский

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The Genome-Wide Identification, Characterization, and Expression Profiles of the NADPH Oxidase (NOX) Gene Family under Drought and Salt Stress in Opisthopappus taihangensis (Asteraceae)

Agronomy, Journal Year: 2024, Volume and Issue: 14(4), P. 653 - 653

Published: March 23, 2024

Plant NAPDH oxidase (NOX) gene family members are critical producers of ROS and play crucial roles in plant signaling, development, stress responses. Opisthopappus taihangensis is a pivotal wild genetic resource Asteraceae. To date, little knowledge exists about the functions NOXs O. taihangensis. In this study, seven typical ferric reduction oxidases (FROs) were identified Dispersed duplication might role expansion NOX/FRO taihangensis, most homologous pairs have undergone purifying selection. Although results collinearity analysis show that these genes relatively conserved, gain loss occurred frequently during evolution Phylogenetic indicated FROs ancient, earliest diverged branch was revealed by maximum likelihood (ML) tree. The Asteraceae divided into six subgroups according to their relationship with Arabidopsis rice members. transcriptome profile unveiled tissue-specific expression patterns complex response modes under drought salt stresses. OtNOX6 OtNOX7 could be recognized as important candidates respond stress. Our provide clues references for further in-depth exploration function other horticulture plants.

Language: Английский

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RNA-seq analysis reveals transcriptome reprogramming and alternative splicing during early response to salt stress in tomato root

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 20, 2024

Salt stress is one of the dominant abiotic conditions that cause severe damage to plant growth and, in turn, limiting crop productivity. It therefore crucial understand molecular mechanism underlying root responses high salinity as such knowledge will aid efforts develop salt-tolerant crops. Alternative splicing (AS) precursor RNA important processing steps regulate gene expression and proteome diversity, consequently, many physiological biochemical processes plants, including stresses like salt stress. In current study, we utilized high-throughput RNA-sequencing analyze changes transcriptome characterize AS landscape during early response tomato Under conditions, 10,588 genes were found be differentially expressed, those involved hormone signaling transduction, amino acid metabolism, cell cycle regulation. More than 700 transcription factors (TFs), members MYB, bHLH, WRKY families, potentially regulated events greatly enhanced under stress, where exon skipping was most prevalent event. There 3709 identified alternatively spliced (DAS), prominent which serine/threonine protein kinase, pentatricopeptide repeat (PPR)-containing protein, E3 ubiquitin-protein ligase. 100 DEGs implicated spliceosome assembly, may salt-responsive roots. This study uncovers stimulation provides a valuable resource for future studies improve tolerance.

Language: Английский

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Exogenous γ-Aminobutyric Acid (GABA) Regulates the Response of Sugar Beet Seedlings to Salt Stress Through GABA Branched Metabolism

Journal of Plant Growth Regulation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Language: Английский

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