Integrated Metabolomic and Transcriptomic Analyses Reveal the Regulatory Mechanism Underlying the Accumulation of Anthocyanins in Cornus officinalis pericarp DOI Creative Commons
Yue Qin,

Xuanmeng Chen,

Jiahui Yang

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(6), P. 651 - 651

Published: June 19, 2024

The mature flesh of Cornus officinalis exhibits a vibrant red color, attributed to its rich anthocyanin content, imparting significant edible and medicinal value. Vibrant colors not only enhance the visual allure materials but also maintain close association with their intrinsic quality. However, intricate process pigment formation governing accumulation in pericarp remains poorly understood. In this study, we conducted comprehensive sampling analysis tissues at three distinct developmental stages, employing morphological-structure observation metabolomic transcriptomic techniques. Our findings reveal substantial increase during transition stage fruit maturation. Metabolomic profiling identified highest expression levels Cyanidin-3-O-glucoside Pelargonidin-3-O-rutinoside stage, suggesting coloration fruit. Through RNA sequencing, 25,740 differentially expressed genes (DEGs), including 41 DEGs associated biosynthesis. correlation between DEG content was explored, further elucidating regulatory network. Additionally, validated pivotal role candidate gene BZ1 synthesis through qRT-PCR, confirming crucial impact on accumulation. This study provides preliminary insights into officinalis, laying foundation for future development new cultivars enhanced contents.

Language: Английский

Comprehensive transcriptome and metabolomic analyses reveal the browning mechanism of walnut explants DOI Creative Commons
Hui Wang, Zhuo Chen, Xiuhong An

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120447 - 120447

Published: Jan. 8, 2025

Language: Английский

Citations

0
Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Lagerstroemia indica petals DOI Creative Commons

Xie Xian,

Tiantian Cheng, Yu Yan

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 12, 2025

Lagerstroemia indica is an ornamental plant with high economic and ecological values. However, the mechanism underlying regulation of L. petal color variation remains unclear. We used LC-MS/MS RNA-seq to uncover genetic basis flower colour help metabolome transcriptome three varieties different colored petal, 'Huahai', 'Zhuimeng', 'Zixian'. A total 54 anthocyanidins were identified, 11 peonidin derivatives, 10 cyanidin 9 delphinidin 5 petunidin malvidin 4 pelargonidin 2 procyanidin 8 other flavonoids. Delphinidin-3-O-glucoside, Malvidin-3-O-glucoside, Petunidin-3-O-glucoside abundant in petals all varieties. 28 differentially accumulated metabolites, including peonidin, cyanidins flavonoids, common 'Zixian' vs. 'Huahai' 'Zhuimeng' 'Huahai'. Peonidin-3-O-glucoside varied greatly among 9923 genes found be expressed between red petals. The upregulation expression ANS (LOC116213234), CHI (LOC116194939), (LOC116196656), F3H (LOC116211316), F3'H (LOC116198721), UGT (LOC104445830) (LOC116210262) was consistent contents kaempferol naringenin, derivatives derivatives. Regarding transcription factors associated anthocyanin pthway, MYB gene family had highest number DEGs, followed by bHLH family, bZIP WRKY MADS box family. Malvidin-3-O-glucoside may contribute ANS, CHI, F3H, involved biosynthesis pigmentation variety. Expression twelve factor has a strongly positive correlation structural genes. Collectively, our results facilitate breeding cultivation enhanced pigmentation.

Language: Английский

Citations

0
Transcriptomic and metabolomic insights reveal the mechanisms underlying varied flower colors on a single wintersweet tree DOI Creative Commons

Huafeng Wu,

Bin Liu, Siqi He

et al.

Scientia Horticulturae, Journal Year: 2025, Volume and Issue: 343, P. 114076 - 114076

Published: March 1, 2025

Language: Английский

Citations

0
Integrated metabolomics and transcriptomics unravel the biosynthZaesis mechanism of anthocyanin in postharvest red raspberry (Rubus idaeus L.) DOI Creative Commons
Huajun Sun, F.Z. Cui, Ying Liu

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

Introduction Anthocyanins are crucial secondary metabolites that responsible for pigment deposition in fruits. Raspberry fruit color shifts from white to red during natural or postharvest ripening. However, the precise mechanisms and biosynthetic pathways of anthocyanins raspberries remain unclear. Methods This study used metabolomic transcriptomic analyses explore anthocyanin biosynthesis at various stages: (RBT-1), white-to-pink (RBT-2), pink (RBT-3), (RBT-4), deep (RBT-5). Results We identified 43 key metabolites, 13,239 DEGs linked raspberry colour development, including cyanidin-3-O-sophoroside cyanidin-3-O-glucoside. The DAMs colored were gentiobioside, pelargonidin-3,5-O-diglucoside, cyanidin-3-O-sambubioside, pelargonidin-3-O-sambubioside. Transcriptome analysis revealed 32 differentially expressed structural genes flavonoid synthesis, with significant upregulation PAL, CHS, F3H, C4H, F3'H, DFR, ANS, CHI , UFGT genes, which promote synthesis accumulation. Integrated showed was correlated 9 involved biosynthesis, 19 transcription factors (TFs), 14 hormone signaling-related genes. Discussion explored regulatory MYB, WRKY, bHLH, NAC factors, as well phytohormone-related modulating metabolism changes raspberries. findings provide valuable insights optimizing storage conditions enhancing quality.

Language: Английский

Citations

0
Integrated Metabolomic and Transcriptomic Analyses Reveal the Regulatory Mechanism Underlying the Accumulation of Anthocyanins in Cornus officinalis pericarp DOI Creative Commons
Yue Qin,

Xuanmeng Chen,

Jiahui Yang

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(6), P. 651 - 651

Published: June 19, 2024

The mature flesh of Cornus officinalis exhibits a vibrant red color, attributed to its rich anthocyanin content, imparting significant edible and medicinal value. Vibrant colors not only enhance the visual allure materials but also maintain close association with their intrinsic quality. However, intricate process pigment formation governing accumulation in pericarp remains poorly understood. In this study, we conducted comprehensive sampling analysis tissues at three distinct developmental stages, employing morphological-structure observation metabolomic transcriptomic techniques. Our findings reveal substantial increase during transition stage fruit maturation. Metabolomic profiling identified highest expression levels Cyanidin-3-O-glucoside Pelargonidin-3-O-rutinoside stage, suggesting coloration fruit. Through RNA sequencing, 25,740 differentially expressed genes (DEGs), including 41 DEGs associated biosynthesis. correlation between DEG content was explored, further elucidating regulatory network. Additionally, validated pivotal role candidate gene BZ1 synthesis through qRT-PCR, confirming crucial impact on accumulation. This study provides preliminary insights into officinalis, laying foundation for future development new cultivars enhanced contents.

Language: Английский

Citations

0