Impact of porcine brush border membrane enzymes on INFOGEST in vitro digestion model: A step forward to mimic the small intestinal phase DOI Creative Commons
Luigia Di Stasio, Salvatore De, Serena Marulo

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 197, P. 115300 - 115300

Published: Nov. 5, 2024

Brush border membrane (BBM) enzymes greatly affect the bioaccessibility and bioavailability of food nutrients. Despite their physiological importance, a step simulating final stage intestinal digestion has not yet been included in harmonized protocols for vitro digestion, primarily due to challenges replicating dynamics degradation. Herein, we propose an advancement toward more physiologically relevant method, complementing static gastric-duodenal INFOGEST model with missing small phase. BBM hydrolase activity, incubation time, at pH 7.2 were established reproduce conditions. Skim milk powder, as protein food, was subjected digestion. Immediately after duodenal phase, digesta supplemented vesicles purified from pig jejunum. To comply dynamic nature balance spontaneous inactivation hydrolases, supplements added every two hours throughout 6 h time. Peptide degradation monitored each by amino acid analysis, free α-amino group assay, HPLC, LC-MS/MS. Hydrolysis peptidases led significant increase acids, reflecting known level adsorption (>90 %) humans eating proteins. LC-MS/MS analysis demonstrated that hydrolases erode progressively peptides released gastro-duodenal processing up stable sequence motifs. The approach described is particularly when endpoint identifying peptide sequences cannot be further hydrolysed digestive or determine bio-accessibility.

Language: Английский

Optimization, validation, and greenness assessment of a capillary zone electrophoresis method for analysis of inorganic and organic anions in Human Milk Oligosaccharides DOI
Minoo Afshar,

Dóra Molnár-Gábor

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127607 - 127607

Published: Jan. 16, 2025

Language: Английский

Citations

0
Bovine Milk Fat Globule Membrane Supplementation and Neurocognitive Development: A Systematic Review and Meta-Analysis DOI Open Access
Therdpong Thongseiratch, Kulnipa Kittisakmontri,

Nutthaporn Chandeying

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(14), P. 2374 - 2374

Published: July 22, 2024

Given the limited evidence, there is no conclusive proof of neurocognitive benefits bovine milk fat globule membrane supplementation in infant formula. This study evaluates formula, comparing it to standard formula and assessing its noninferiority breast milk. Data were sourced from studies published between January 2000 March 2024 PubMed, Cochrane Library, Web Science, Embase. Eight randomized controlled trials involving 1352 healthy term neonates, infants, children up 2 years old included. Bovine was significantly associated with improved cognitive development (mean difference: 3.29, 95% CI: 1.65 4.93, p < 0.001) demonstrated minimal heterogeneity (I2 = 0%, 0.564). It showed significant improvement executive function but not language, motor, or social-emotional development. In non-inferiority analysis, difference compared regarding These findings support as a valuable addition for benefits.

Language: Английский

Citations

2
Impact of porcine brush border membrane enzymes on INFOGEST in vitro digestion model: A step forward to mimic the small intestinal phase DOI Creative Commons
Luigia Di Stasio, Salvatore De, Serena Marulo

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 197, P. 115300 - 115300

Published: Nov. 5, 2024

Brush border membrane (BBM) enzymes greatly affect the bioaccessibility and bioavailability of food nutrients. Despite their physiological importance, a step simulating final stage intestinal digestion has not yet been included in harmonized protocols for vitro digestion, primarily due to challenges replicating dynamics degradation. Herein, we propose an advancement toward more physiologically relevant method, complementing static gastric-duodenal INFOGEST model with missing small phase. BBM hydrolase activity, incubation time, at pH 7.2 were established reproduce conditions. Skim milk powder, as protein food, was subjected digestion. Immediately after duodenal phase, digesta supplemented vesicles purified from pig jejunum. To comply dynamic nature balance spontaneous inactivation hydrolases, supplements added every two hours throughout 6 h time. Peptide degradation monitored each by amino acid analysis, free α-amino group assay, HPLC, LC-MS/MS. Hydrolysis peptidases led significant increase acids, reflecting known level adsorption (>90 %) humans eating proteins. LC-MS/MS analysis demonstrated that hydrolases erode progressively peptides released gastro-duodenal processing up stable sequence motifs. The approach described is particularly when endpoint identifying peptide sequences cannot be further hydrolysed digestive or determine bio-accessibility.

Language: Английский

Citations

1