Development of an immunoassay for the detection and diagnosis of microbially-influenced corrosion caused by methanogenic Archaea DOI
Sven Lahme,

Jorge Mantilla Aguas

CORROSION, Journal Year: 2024, Volume and Issue: 81(1), P. 21 - 26

Published: Dec. 13, 2024

Microbially-influenced corrosion (MIC) is a costly problem across several industries. The steadily rising use of advanced molecular biological methods to investigate MIC allowed ever deeper insights into the underlying microbial community structure and function. However, currently available technologies do not allow accurate detection diagnosis in field. recent discovery special hydrogenase certain corrosive methanogenic Archaea for development first-ever biomarker termed here micH. micH gene encodes large subunit [NiFe] involved MIC. Here we describe recombinant antibody that enables specific sensitive MicH protein Western blot immunoassays. Using determined lower limit per assay be around 0.3 fg MicH. immunoassay was able detect strong signal micH-positive pure cultures Methanobacterium-like. strain IM1 cultivated on iron granules, over 500 times micH-negative Methanococcus maripaludis S2 culture. To further evaluate ability differentiate from noncorrosive communities, tested ten oil field enrichment showed wide range rates (0.02 mm/y 0.48 mm/y). We detected planktonic (36.5 pg/mL 1473.5 pg/mL) carbon steel biofilm samples (41.0 pg/cm2 7971.3 pg/cm2) enrichments (0.17 mm/y) but did any tests (<0.08 despite activity. results indicate likely caused by expressing newly developed MicH-specific can monitor their immunoassays allows scientists practitioners activity Archaea.

Language: Английский

Recent advances in Surface-Enhanced Raman Scattering (SERS) for the diagnosis, treatment, and prognosis of ovarian cancer DOI

Biqing Chen,

Xiaohong Qiu,

Yang Li

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112787 - 112787

Published: Jan. 1, 2025

Language: Английский

Citations

1
Glycoconjugate Vaccine Quantification: An Overview on Present and Future Trends in Analytical Development DOI

Giovanni Santostefano,

Alessio Corrado,

Carmine Malzone

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Language: Английский

Citations

0
Precision oncology through SERS: emerging approaches for improved cancer diagnosis and prognosis DOI
Eduardo García‐Rico, Miguel A. Correa‐Duarte, Ramón A. Álvarez‐Puebla

et al.

Nanomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 4

Published: April 28, 2025

Language: Английский

Citations

0
Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 DOI Creative Commons
Mengmeng Zhao, Lv Chen,

Jiankun Pang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 18, 2025

The non-structural protein 4 (NSP4) of porcine reproductive and respiratory syndrome virus (PRRSV) is equipped with 3C-like serine protease (3CLSP) activity, influencing crucial aspects such as replication, host IFN-β suppression, cell apoptosis induction, PRRSV detection facilitation. In response to wild or attenuated strains, antibodies against proteins are generated, while inactivated vaccines fail elicit responses. Employing the Enzyme-Linked Immunosorbent Assay (ELISA) method targeting helps discern immune effects versus vaccine strains. study focused on NSP4 from XH-GD strain (GenBank No. EU624117.1), which was cloned, expressed, leveraged a coating for establishing an indirect enzyme-linked immunosorbent assay method. This showcased outstanding specificity, repeatability, sensitivity, exhibiting notable agreement rate 91.74% IDEXX ELISA kit. successful development not only supports but also provides robust platform ongoing antibody monitoring in pig farming. Utilizing offers more sustainable approach continuous surveillance. high between this commercial kits lays solid groundwork effectively differentiating vaccines, enhancing management populations.

Language: Английский

Citations

0
Evaluation of a multiplexed assay for six urinary proteins based on liquid microarray analysis technology DOI Creative Commons
Peng Zhang, Yi Liu, Xin Zhou

et al.

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115820 - 115820

Published: Feb. 1, 2025

Language: Английский

Citations

0
CRISPR/Cas‐SERS Sensing Platforms: A Frontier Technology for Next‐Generation Fast, Low‐Cost, Ultra‐Micro Biosample Detection DOI
Chengxin Bao, Xiangguo Liu, Chongyang Liang

et al.

Journal of Raman Spectroscopy, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

ABSTRACT Researchers have long been interested in nucleic acid detection technology. Surface‐enhanced Raman spectroscopy (SERS) is distinguished by its high sensitivity, minimal sample volume, resistance to fluorescence interference, cost‐effectiveness, and rapidity compared conventional analysis methods. The clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas), a novel gene editing tool, has garnered significant interest due precise identification isothermal advantages. Integrating the CRISPR/Cas system's specific capabilities with high‐sensitivity fingerprinting properties of SERS offers sensitive, ultra‐low rapid, straightforward method for detecting new modalities. This review delineates components characteristics system, encompassing three Cas proteins (Cas9, Cas12, Cas13) technologies derived from CRISPR/Cas, namely, enzymatic reporter unlocking (SHERLOCK) DNA endonuclease‐targeted CRISPR trans reporters (DETECTR). Advancements CRISPR/Cas‐SERS‐based assays were emphasized, traditional non‐nucleic tests. Examples encompass microfluidics/microdroplet‐based CRISPR/Cas‐SERS non‐amplified based on CRISPR/Cas‐SERS, so on.

Language: Английский

Citations

0
Electrochemical Biosensors for Blood‐borne Viruses Detection: A review DOI

Kiana Ketabi,

Arash Mohammadinejad, Hoorieh Soleimanjahi

et al.

ChemNanoMat, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

Abstract This review article discusses the advancements in electrochemical biosensors for detecting blood‐borne viruses. Infections like HBV, HCV, HIV, and HTLV transmitted through blood can be life‐threatening have significant impact on both health social life. Therefore, timely diagnosis of these infections is crucial. Various effective diagnostic methods been developed to detect infections. The combination nanotechnology biology has led creation new techniques using electrodes microorganisms. aims summarize recent studies performance viruses last five years, comparing their characteristics, limits detection, other relevant parameters.

Language: Английский

Citations

2
Design of novel electrochemical sensor functionalized with green nanoparticles for tetracycline monitoring DOI

Manahil Babiker Elamin

Polymers for Advanced Technologies, Journal Year: 2024, Volume and Issue: 35(8)

Published: Aug. 1, 2024

Abstract Increasing the release of antibiotics in particular tetracycline environment is become one most concerns that threat human and animals' health. Regarding their low cost, sensitivity, portability, electrochemical sensors have received attention for monitoring. With a nanostructure, diverse active functional sites, good conductivity, green nanoparticles are considered now as an attractive material design. The objective present paper then design sensor functionalized with based on gum Arabic detection. synthesized by direct reduction silver nanoparticle polymer. intrinsic properties obtained examined using different techniques namely UV–Vis absorption (UV), X‐ray diffraction (XRD), transmission electron microscopy (TEM), thermogravimetric TGA. To follow up, modification well detection, cyclic voltammetry (CV) square wave carried out. Correlation between results demonstrated dispersion homogeneity nanoparticles, applicability measurements. In fact, has wide concentrations range from 0.1 to 1250 nM limit detection order 0.056 nM, lower than those reported literature. selectivity test, investigated against various interfering similar structure tetracycline, proven discrimination all molecules. addition, was successfully applied real samples, found recovery rates ranging 90.4% 106.9%. confirm could commercially be viable next generation also variety pharmaceutical pollutants environment.

Language: Английский

Citations

2
Immuno‐Rolling Circle Amplification (Immuno‐RCA): Biosensing Strategies, Practical Applications, and Future Perspectives DOI
Limei Zhang, Hao Bai, Jie Zou

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract In the rapidly evolving field of life sciences and biomedicine, detecting low‐abundance biomolecules, ultraweak biosignals presents significant challenges. This has spurred a rapid development analytical techniques aiming for increased sensitivity specificity. These advancements, including signal amplification strategies integration biorecognition events, mark transformative era in bioanalytical precision accuracy. A prominent method among these innovations is immuno‐rolling circle (immuno‐RCA) technology, which effectively combines immunoassays with via RCA. process starts when targeted biomolecule, such as protein or cell, binds to an immobilized antibody probe on substrate. The introduction circular DNA template triggers RCA, leading exponential significantly enhanced intensity, thus target molecule detectable quantifiable even at single‐molecule level. review provides overview biosensing strategy extensive practical applications immuno‐RCA biomarkers. Furthermore, it scrutinizes limitations inherent sensors sets forth expectations their future trajectory. serves valuable reference advancing various domains, diagnostics, biomarker discovery, molecular imaging.

Language: Английский

Citations

1
Evaluation and Limitations of the Novel Chemiluminescent Enzyme Immunoassay Technique for Measuring Total Tau Protein in the Cerebrospinal Fluid of Patients with Human Prion Disease: A 10-Year Prospective Study (2011–2020) DOI Creative Commons

Kong Weijie,

Toshiaki Nonaka,

Katsuya Satoh

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(14), P. 1520 - 1520

Published: July 15, 2024

Recently, the investigation of cerebrospinal fluid (CSF) biomarkers for diagnosing human prion diseases (HPD) has garnered significant attention. Reproducibility and accuracy are paramount in biomarker research, particularly measurement total tau (T-tau) protein, which is a crucial diagnostic marker. Given global impact coronavirus disease pandemic, frequency measuring this protein using one world's fully automated assays, chemiluminescent enzyme immunoassay (CLEA), increased. At present, diagnosis monitoring neurological mainly rely on traditional methods, but their responsiveness limited. There limited knowledge CLEA measurements. We aimed to measure T-tau elucidate its merits limitations.

Language: Английский

Citations

1