α-Chitosan and β-Oligochitosan Mixtures-Based Formula for In Vitro Assessment of Melanocyte Cells Response

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6768 - 6768

Published: June 20, 2024

Chitosan is a natural polymer with numerous biomedical applications. The cellular activity of chitosan has been studied in various types cancer, including melanoma, and indicates that these molecules can open new perspectives on antiproliferative action anticancer therapy. This study analyzes how different conformations, such as α-chitosan (CH) or β-oligochitosan (CO), degrees deacetylation (DDA) molar mass (MM), both concentrations CH–CO mixtures, influence the processes SK-MEL-28 melanocytes, to estimate reactivity cells applied treatments. vitro evaluation was carried out, aiming at metabolism (MTT assay), morphology, chitinase-like glycoprotein YKL-40 expression. effect mixture application melanocytes obvious low α-chitosan/β-oligochitosan (1:2 ratio), cell’s response supporting hypothesis β-oligo-chitosan amplifies effect. oligochitosan mixture, favored by β conformation its small size, penetrates faster into cells, being more reactive when interacting some components. Morphological effects expressed loss cell adhesion depletion synthesis are significant responses melanocytes. (1.5 kDa) induces an extension cytophysiological limits viability compared (400–900 kDa). Statistical analysis using multivariate techniques showed differences between CH samples mixtures.

Language: Английский

Exploring the Multifaceted Effects of Silymarin on Melanoma: Focusing on the Role of Lipid-Based Nanocarriers

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 99, P. 105950 - 105950

Published: Sept. 1, 2024

Language: Английский