Vitamin D Receptor and CYP450 Enzyme Dysregulation May Mediate Oral Cancer Responsiveness

Targets, Journal Year: 2025, Volume and Issue: 3(1), P. 6 - 6

Published: Feb. 8, 2025

Many health benefits are associated with Vitamin D (VitD), although deficiency is poor outcomes and the increased risk of cancer development. For example, many tissue-specific enzymes involved in VitD metabolism, mutations or deletions within receptor (VDR) genes known to increase by altering their functions bioavailability, less about these phenomena oral cancers. Using well-characterized, commercially available cell lines (OKF4, HGF-1, SCC4, SCC9, SCC15, SCC25, CAL27), mRNA expression P450 cytochrome metabolic qPCR revealed differential results. One line (SCC15) did not express either FOK1 polymorphism was also least affected VitD3 administration growth assays. In contrast, most cancers were missing one more hydrolase (CYP2R1 CYP24A1) hydrolate (CYP27A1 CYP27B1) enzymes. SCC25 both inhibited assays, while SCC4 hydroxylase VitD2. These associations between (or lack thereof) VitD2 responsiveness can be used identify molecular targets, which may lead effective screening tools for VitD-related, complementary alternative therapies.

Language: Английский

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Comprehension of PTEN-Regulated MicroRNA Profiling in Oral Premalignant Lesions: A Critical Link to Early Detection of Oral Squamous Cell Carcinoma

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Oral premalignant lesions represent the most prevalent observed within oral cavity. These generally remain asymptomatic until they advance to more severe stages, at which point their progression squamous cell carcinoma (OSCC) becomes evident. Addressing this transformation necessitates further investigative efforts. The PTEN (phosphatase and tensin homolog) gene functions as a crucial tumor suppressor, with its expression regulated by variety of complex mechanisms, including transcriptional, post-transcriptional, post-translational processes. MicroRNAs (miRNAs) are significant modulators play an integral role in transition from OSCC. This review evaluates impact miRNA dysregulation on examines continuum model suppression. It posits that loss functionality may transpire without alterations DNA sequence, particularly through mechanisms associated regulation. Furthermore, discourse elucidates structural interactions between miRNAs, context lesions, influence rate Such insights for informing treatment strategies. also explores potential targeting specific miRNAs restore functionality, intending improve clinical outcomes patients diagnosed By elucidating these regulatory interactions, analysis aims identify pathways conducive development targeted therapeutic

Language: Английский

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Downstream Target Analysis for miR-365 among Oral Squamous Cell Carcinomas Reveals Differential Associations with Chemoresistance

Life, Journal Year: 2024, Volume and Issue: 14(6), P. 741 - 741

Published: June 10, 2024

Expression of microRNAs, such as miR-365, is known to be dysregulated in many tumors, including oral cancers, although little about their role or functions. The objective this project evaluate the downstream targets miR-365 determine any potential pathways effects. Downstream for (miRdatabase target scores > 90) were used qPCR screening cancer cell lines (SCC4, SCC9, SCC15, SCC25, CAL27). Each line expressed molybdenum cofactor synthesis-2 (MOCS2), erythropoietin receptor (EPOR), IQ motif containing-K (IQCK), carboxypeptidase A3 (CPA3), solute carrier family 24 member-3 (SLC24A3), and coiled-coil domain containing 47 (CCDC47)-although expression levels varied somewhat. However, differential results observed with ubiquitin protein ligase E3 component n-recognin-3 (UBR3), nudix hydrolase-12 (NUDT12), zinc finger CCHC-type containing-14 (ZCCHC14), homeobox leucine zipper encoding (HOMEZ). These data suggest that are cancers screened, UBR3, ZCCHC14, HOMEZ, NUDT12, which may correlated chemoresistance among two specific (SCC25, SCC9). signal patient treatment tumors exhibiting chemotherapeutic resistance.

Language: Английский

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Differential Expression of DNA Methyltransferase (DNMT1 and DNMT3), Histone Deacetylase (HDAC1 and HDAC2), and Upstream Target Regulators MiR-145 and Mir-152 among Oral Cancers

Targets, Journal Year: 2024, Volume and Issue: 2(3), P. 224 - 236

Published: Aug. 18, 2024

Epigenetic modulation of DNA and histones facilitated by histone deacetylases (HDAC) is associated with the development progression many cancers, although less known about methyltransferase (DNMT) in oral cancers regulation these targets. Using commercially available cell lines, squamous carcinomas (SCC4, SCC9, SCC15, SCC25, CAL27), normal gingival fibroblasts (HGF-1), growth assays mRNA expression were evaluated using ANOVA. These results revealed homeostasis enzyme DNMT1 was significantly higher among slow-growing HGF-1 cells than fast-growing p < 0.05. In contrast, DNMT3A DNMT3B compared cells, However, differential HDAC1 HDAC2 observed SCC4, CAL27 cells. Further analysis miR-152 (regulation control DNMT expression) miR-21, miR-221, miR-145 HDAC all produced but none miR-221. (SCC15) (SCC25) suggested alternative epigenetic pathways mechanisms may be responsible for some observations this study.

Language: Английский

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Synergistic Therapeutic Effects of Stimuli Responsive Nanocomposites for Eculizumab Delivery on Membranous Glomerulonephritis

Journal of Inorganic and Organometallic Polymers and Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Language: Английский

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