Transcriptome Profiling and Weighted Gene Correlation Network Analysis Reveal Hub Genes and Pathways Involved in the Response to Polyethylene-Glycol-Induced Drought Stress of Two Citrus Rootstocks DOI Creative Commons
Emanuele Scialò, Angelo Sicilia, Alberto Continella

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 595 - 595

Published: Aug. 7, 2024

Agriculture faces the dual challenge of increasing food production and safeguarding environment. Climate change exacerbates this challenge, reducing crop yield biomass due to drought stress, especially in semi-arid regions where Citrus plants are cultivated. Understanding molecular mechanisms underlying tolerance is crucial for developing adaptive strategies. Plants two citrus rootstocks, Carrizo Citrange Bitters (C22), were grown aerated half-strength Hoagland’s nutrient solution. Post-acclimation, exposed a solution containing 0% (control) or 15% PEG-8000 10 days. Leaf malonyl dialdehyde (MDA) hydrogen peroxide (H2O2) content measured assess reached oxidative stress level. Total RNA was extracted, sequenced, de novo-assembled. Weighted Gene Correlation Network Analysis (WGCNA) conducted examine relationship between gene expression patterns levels MDA H2O2 used as indicators. Plant visual inspection contents clearly indicate that more tolerant than towards PEG-induced stress. RNA-Seq analysis revealed significantly higher number differentially expressed genes (DEGs) (6092) (320), with most being associated sensing, ROS scavenging, osmolyte biosynthesis, cell wall metabolism. Moreover, WGCNA identified transcription factors correlated levels, thus providing insights into drought-coping strategies offering candidate enhancing tolerance.

Language: Английский

Identification of Key Pathways and Candidate Genes Controlling Organ Size Through Transcriptome and Weighted Gene Co-Expression Network Analyses in Navel Orange Plants (Citrus sinensis) DOI Open Access

Yi Lin,

Jinghao Zheng, Quan Wan

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 259 - 259

Published: Feb. 23, 2025

Background/Objectives: Organ size is a critical target trait in fruit-tree breeding programs, as it significantly impacts the economic value of plants by influencing their biomass, yield, and quality. Understanding molecular mechanisms underlying organ citrus essential for new cultivars with superior fruit Methods: In this study, we investigated regulatory network involved using Citrus sinensis ‘Newhall’ navel orange variety its large-organ mutant, ‘M25’. Results: Ploidy analysis indicated that enlargement observed ‘M25’ was not attributable to changes chromosome ploidy. Furthermore, RNA sequencing tender leaves young fruits from both oranges identified 1817 1605 differentially expressed genes (DEGs), respectively. Functional enrichment revealed these DEGs were enriched pathways associated regulation, including those related cell division, DNA replication, protein biosynthesis, plant hormone signal transduction, wall metabolism. Weighted gene co-expression grey 60 modules key enlargement; modules, 51 35 hub genes, Combined homologous function annotation expression four transcription-factor-encoding (Cs_ont_6g005380, Cs_ont_8g025330, Cs_ont_9g019400, Cs_ont_9g008010) candidate potentially size. Conclusions: Among these, Cs_ont_8g025330 (CsMYB73) inferred be through auxin cytokinin regulation. These findings lay foundation further investigations mechanism varieties.

Language: Английский

Citations

0
Integration of RNAseq and WGCNA to study phenotypic plasticity in wine grape cultivars grown in central-southern Italy DOI
Angelo Sicilia,

C.S. Taranto,

Filippo Ferlito

et al.

Acta Horticulturae, Journal Year: 2025, Volume and Issue: 1418, P. 173 - 184

Published: Feb. 1, 2025

Language: Английский

Citations

0
Biocontrol activity of novel and known bioproducts based on Bacillus strains and basic products against Botrytis cinerea in tomato fruit DOI Creative Commons
S. Panebianco,

S. Americo,

Angela Roberta Lo Piero

et al.

Biological Control, Journal Year: 2025, Volume and Issue: unknown, P. 105780 - 105780

Published: April 1, 2025

Language: Английский

Citations

0
Biocontrol efficacy of Pseudomonas mediterranea PVCT 3C against Plenodomus tracheiphilus: In vitro and in planta mechanisms at early disease stages DOI Creative Commons
Giulio Dimaria, Angelo Sicilia, Francesco Modica

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 287, P. 127833 - 127833

Published: July 16, 2024

In this study, we investigated the biocontrol activity of P. mediterranea strain PVCT 3C against Mal secco, a severe disease citrus caused by vascular fungus Plenodomus tracheiphilus. vitro, bacterial diffusible compounds, volatile organic compounds and culture filtrates produced reduced mycelial growth conidial germination tracheiphilus, also affecting pigmentation. The application suspensions leaf-spraying before inoculation with pathogen on plants highly susceptible species sour orange lemon led to an overall reduction in incidence index, above all during early stage. genome was subjected whole-genome shotgun sequencing study molecular mechanisms action strain. silico annotation biosynthetic gene clusters for secondary metabolites revealed presence numerous encoding antimicrobial (e.g. cyclic lipopeptides, hydrogen cyanide, siderophores) candidate novel products. During asymptomatic phase (seven days post-inoculation), treatments interfered expression different fungal genes, as assessed NGS de novo assembly RNA-seq approach. These results suggest that or its may offer potential effective sustainable alternative contain tracheiphilus infections via integrated management.

Language: Английский

Citations

2
Study of red vine phenotypic plasticity across central-southern Italy sites: an integrated analysis of the transcriptome and weather indices through WGCNA DOI Creative Commons
Angelo Sicilia, Clizia Villano, Riccardo Aversano

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 11, 2024

The grapevine (

Language: Английский

Citations

2
Transcriptome Profiling and Weighted Gene Correlation Network Analysis Reveal Hub Genes and Pathways Involved in the Response to Polyethylene-Glycol-Induced Drought Stress of Two Citrus Rootstocks DOI Creative Commons
Emanuele Scialò, Angelo Sicilia, Alberto Continella

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 595 - 595

Published: Aug. 7, 2024

Agriculture faces the dual challenge of increasing food production and safeguarding environment. Climate change exacerbates this challenge, reducing crop yield biomass due to drought stress, especially in semi-arid regions where Citrus plants are cultivated. Understanding molecular mechanisms underlying tolerance is crucial for developing adaptive strategies. Plants two citrus rootstocks, Carrizo Citrange Bitters (C22), were grown aerated half-strength Hoagland’s nutrient solution. Post-acclimation, exposed a solution containing 0% (control) or 15% PEG-8000 10 days. Leaf malonyl dialdehyde (MDA) hydrogen peroxide (H2O2) content measured assess reached oxidative stress level. Total RNA was extracted, sequenced, de novo-assembled. Weighted Gene Correlation Network Analysis (WGCNA) conducted examine relationship between gene expression patterns levels MDA H2O2 used as indicators. Plant visual inspection contents clearly indicate that more tolerant than towards PEG-induced stress. RNA-Seq analysis revealed significantly higher number differentially expressed genes (DEGs) (6092) (320), with most being associated sensing, ROS scavenging, osmolyte biosynthesis, cell wall metabolism. Moreover, WGCNA identified transcription factors correlated levels, thus providing insights into drought-coping strategies offering candidate enhancing tolerance.

Language: Английский

Citations

1