Comparative transcriptomic and proteomic analyses of two salt-tolerant alfalfa (Medicago sativa L.) genotypes: investigation of the mechanisms underlying tolerance to salt DOI Creative Commons

J Hang,

Ting Song,

Ling Zhang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 13, 2024

Abiotic stressors such as salt stress restrict plant development and output, which lowers agricultural profitability. In this study, alfalfa ( Medicago sativa L.) varieties with different levels of tolerance were examined using high-throughput RNA sequencing (RNA-Seq) Tandem Mass Tags (TMT) technologies to study the reactions root systems stress, from transcriptomics proteomics perspectives. The Atlantic (AT) Zhongmu-1 (ZM-1) selected evaluated after 2 h 6 treatment 150 mM NaCl. results showed that under for h, 1810 differentially expressed genes (DEGs) 160 proteins (DEPs) in AT screened, while 9341 DEGs 193 DEPs screened ZM-1. Under 7536 118 AT, 11,754 190 Functional annotation pathway enrichment analyses indicated DEGS mainly involved glutathione metabolism, biosynthesis secondary metabolites, glycolysis/gluconeogenesis, carbon fixation photosynthetic organisms, photosynthesis pathways. A series related also identified, including GSTL3 GSTU3 GST gene family, PER5 PER10 , PER APR COMT are metabolites. This provides insights into resistance mechanisms plants, metabolic pathways identified may be helpful breeding future.

Language: Английский

Caffeic acid O-methyltransferase from Ligusticum chuanxiong alleviates drought stress, and improves lignin and melatonin biosynthesis DOI Creative Commons

Enxia Huang,

Jie Tang,

Simin Song

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 18, 2024

Drought stress is a major constraint on plant growth and agricultural productivity. Caffeic acid O-methyltransferase (COMT), an enzyme involved in the methylation of various substrates, plays pivotal role responses to abiotic stress. The involvement COMTs drought response, particularly through enhancement lignin melatonin biosynthesis, remains poorly understood. In this study, LcCOMT was firstly proposed be associated with biosynthesis both melatonin, as demonstrated sequence comparison, phylogenetic analysis, conserved motif identification. vitro enzymatic assays revealed that effectively methylates N-acetylserotonin albeit higher K m value compared caffeic acid. Site-directed mutagenesis residues Phe171 Asp269 resulted significant reduction catalytic activity for acid, minimal impact N-acetylserotonin, underscoring specificity these substrate binding catalysis. Under conditions, expression significantly upregulated. Overexpression gene Arabidopsis plants conferred enhanced tolerance, characterized by elevated levels, increased chlorophyll carotenoid content, heightened activities antioxidant enzymes peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), reduced malondialdehyde (MDA) hydrogen peroxide (H 2 O ) accumulation. This study among few demonstrate COMT-mediated tolerance achieved simultaneous promotion biosynthesis. represents first functionally COMT Apiaceae family, it holds potential target genetic future crop improvement strategies.

Language: Английский

Citations

4
Molecular signatures that translate across omics layers and crops under high aluminium and low phosphorus stress facilitate the identification of reliable molecular targets for genotyping in lentil. DOI

Kavya Kadiyala,

Noren Singh Konjengbam,

M James

et al.

PubMed, Journal Year: 2025, Volume and Issue: 25(1), P. 52 - 52

Published: March 5, 2025

Language: Английский

Citations

0
Transmembrane protein, MiRPH1, enhancing plant immunity through SA accumulation and antioxidants DOI
Lei Chen,

Ruixiong Luo,

Huiliang Wang

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102691 - 102691

Published: April 1, 2025

Language: Английский

Citations

0
Transcriptomics–proteomics analysis reveals StCOMT1 regulates drought, alkali and combined stresses in potato DOI
Ren-Zhi ZHANG, Yong Wang, Yichen Kang

et al.

Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(5)

Published: April 29, 2025

Language: Английский

Citations

0
Comparative transcriptomic and proteomic analyses of two salt-tolerant alfalfa (Medicago sativa L.) genotypes: investigation of the mechanisms underlying tolerance to salt DOI Creative Commons

J Hang,

Ting Song,

Ling Zhang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 13, 2024

Abiotic stressors such as salt stress restrict plant development and output, which lowers agricultural profitability. In this study, alfalfa ( Medicago sativa L.) varieties with different levels of tolerance were examined using high-throughput RNA sequencing (RNA-Seq) Tandem Mass Tags (TMT) technologies to study the reactions root systems stress, from transcriptomics proteomics perspectives. The Atlantic (AT) Zhongmu-1 (ZM-1) selected evaluated after 2 h 6 treatment 150 mM NaCl. results showed that under for h, 1810 differentially expressed genes (DEGs) 160 proteins (DEPs) in AT screened, while 9341 DEGs 193 DEPs screened ZM-1. Under 7536 118 AT, 11,754 190 Functional annotation pathway enrichment analyses indicated DEGS mainly involved glutathione metabolism, biosynthesis secondary metabolites, glycolysis/gluconeogenesis, carbon fixation photosynthetic organisms, photosynthesis pathways. A series related also identified, including GSTL3 GSTU3 GST gene family, PER5 PER10 , PER APR COMT are metabolites. This provides insights into resistance mechanisms plants, metabolic pathways identified may be helpful breeding future.

Language: Английский

Citations

0