Plasma Proteins Associated with COVID-19 Severity in Puerto Rico

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5426 - 5426

Published: May 16, 2024

Viral strains, age, and host factors are associated with variable immune responses against SARS-CoV-2 disease severity. Puerto Ricans have a genetic mixture of races: European, African, Native American. We hypothesized that unique proteins/pathways COVID-19 severity in Rico. Following IRB approval, total 95 unvaccinated men women aged 21–71 years old were recruited Rico from 2020–2021. Plasma samples collected COVID-19-positive subjects (n = 39) COVID-19-negative individuals 56) during acute disease. stratified based on symptomatology as follows: mild 18), moderate 13), severe 8). Quantitative proteomics was performed plasma using tandem mass tag (TMT) labeling. Labeled peptides subjected to LC/MS/MS analyzed by Proteome Discoverer (version 2.5), Limma software 3.41.15), Ingenuity Pathways Analysis (IPA, version 22.0.2). Cytokines quantified human cytokine array. Proteomics analyses severely affected revealed 58 differentially expressed proteins. Cadherin-13, which participates synaptogenesis, downregulated patients validated ELISA. Cytokine immunoassay showed TNF-α levels decreased This study uncovers potential predictors new avenues for treatment Ricans.

Language: Английский

Analysis of Sigma-1 Receptor Antagonist BD1047 Effect on Upregulating Proteins in HIV-1-Infected Macrophages Exposed to Cocaine Using Quantitative Proteomics

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 1934 - 1934

Published: Aug. 23, 2024

HIV-1 infects monocyte-derived macrophages (MDM) that migrate into the brain and secrete virus neurotoxic molecules, including cathepsin B (CATB), causing cognitive dysfunction. Cocaine potentiates CATB secretion neurotoxicity in HIV-infected MDM. Pretreatment with BD1047, a sigma-1 receptor antagonist, before cocaine exposure reduces HIV-1, secretion, neuronal apoptosis. We aimed to elucidate intracellular pathways modulated by BD1047 MDM exposed cocaine. hypothesized Sig1R antagonist prior cocaine, significantly deregulates proteins involved replication lead neurotoxicity. culture lysates from HIV-1-infected women treated were compared untreated controls using TMT quantitative proteomics, bioinformatics, Lima statistics, pathway analyses. Results demonstrate pretreatment dysregulated eighty (80) when infected group. found fifteen (15) related infection, CATB, mitochondrial function. Upregulated oxidative phosphorylation (SLC25A-31), mitochondria (ATP5PD), ion transport (VDAC2-3), endoplasmic reticulum (PHB, TMED10, CANX), cytoskeleton remodeling (TUB1A-C, ANXA1). treatment protects upregulating reduce damage, ER transport, exocytosis associated CATB-induced

Language: Английский

ENNG-Mix: Enhanced Semi-Supervised Anomaly Detection with Pseudo-Anomaly Generation

2022 7th International Conference on Intelligent Computing and Signal Processing (ICSP), Journal Year: 2024, Volume and Issue: unknown, P. 421 - 425

Published: April 19, 2024

Language: Английский