Topical nanoencapsulated cannabidiol cream as an innovative strategy combating UV-A–induced nuclear and mitochondrial DNA injury: A pilot randomized clinical study DOI Creative Commons

Erika McCormick,

Haowei Han, Sara Abdel Azim

et al.

Journal of the American Academy of Dermatology, Journal Year: 2024, Volume and Issue: 91(5), P. 855 - 862

Published: July 25, 2024

BackgroundUV-A radiation contributes to photoaging/photocarcinogenesis by generating inflammation and oxidative damage. Current photoprotective strategies are limited the availability/utilization of UV-A filters, highlighting an unmet need. Cannabidiol (CBD), having anti-inflammatory/antioxidant properties via regulation nuclear erythroid 2–related factor, heme oxygenase 1, peroxisome proliferator-activated receptor gamma, could potentially mitigate damage from exposure.Objective/MethodsThis is a prospective, single-center, pilot clinical trial (NCT05279495). Nineteen participants applied nano-CBD (nCBD) or vehicle (VC) cream randomized, blinded buttock sites twice daily for 14 days; then, treated were irradiated with ≤3× minimal erythema dose. After 24 hours, punch biopsies obtained histology, immunohistochemistry, real-time polymerase chain reaction.ResultsAt 21% had less observed on CBD-treated skin than VC skin. Histologically, nCBD-treated reduced UV-A–induced epidermal hyperplasia (P = .01). Immunohistochemistry detected cytoplasmic/nuclear 8-oxoguanine glycosylase 1 staining in compared < Quantitative mtDNA reaction demonstrated that deletion ND4 (proxy:4977 bp deletion; P .003) ND1 (proxy:3895 .002) was significantly vivo nCBD treatment VC.LimitationsSmall sample size this study's limitation.ConclusionTopically formation frequent mutagenic DNA base lesion protected against mutations associated aging. To our knowledge, first identify UV-protective capacity CBD-containing topicals humans. exposure. This reaction. At VC. Small limitation. Topically

Language: Английский

Synthesis of 7,8-dihydroxy-4-phenylbenzo[g]coumarins as potential multitarget anti-skin-aging candidates DOI
Yasser Fakri Mustafa

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1321, P. 139806 - 139806

Published: Aug. 26, 2024

Language: Английский

Citations

10
Hair Growth-Promoting Effects of Astragalus sinicus Extracts in Human Follicle Dermal Papilla Cells DOI Creative Commons

Chae Young Jeon,

Min Young Go,

In-Kyung Kim

et al.

Cosmetics, Journal Year: 2025, Volume and Issue: 12(1), P. 6 - 6

Published: Jan. 4, 2025

Hair loss is influenced by various stresses and disruptions in the hair growth cycle within follicles. The activation of these follicles primarily driven dermal papilla cells, which play a central role progression formation. Many studies are being carried out on natural plants to improve loss. In this study, we assessed potential Astragalus sinicus, known as pharmacological herb, mitigating damage human follicle cells (HFDPCs) caused dihydrotestosterone (DHT). We found that sinicus extracts (ASEs) facilitated wound healing elevated alkaline phosphatase expression DHT-damaged HFDPCs. ASEs also significantly reduced reactive oxygen species (ROS) generated DHT increased ATP levels restoring mitochondrial membrane damaged cells. Furthermore, demonstrated substantially enhanced phosphorylation AKT/ERK pathway activated Wnt signaling Taken together, suggest may be ingredient for enhancing treatment.

Language: Английский

Citations

0
NLRX1 deficiency exacerbates skin inflammation in atopic dermatitis by disrupting mitophagy DOI

Lixin Yue,

Pei Qiao, Xia Li

et al.

Clinical Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110442 - 110442

Published: Jan. 1, 2025

Language: Английский

Citations

0
Enhanced Antioxidant and Protective Effects of Fermented Solanum melongena L. Peel Extracts Against Ultraviolet B-Induced Skin Damage DOI Open Access

Joo Hwa Lee,

Jinsick Kim,

Yu Chang Jo

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(5), P. 847 - 847

Published: Feb. 28, 2025

Background/Objectives: The skin, being the body's outermost organ, plays a vital role in protecting against various external stimuli. Ultraviolet generates reactive oxygen species (ROS), promoting secretion of matrix metalloproteinases (MMPs) and inducing collagen degradation. Many studies have been conducted to identify natural substances that can prevent or delay harmful effects UV. Methods: A wound healing assay, DCF-DA (ROS) JC-1 assay were performed assess bio-converted eggplant peels (BEPs) on human dermal fibroblasts (HDFs). Western blot analysis was also understand underlying mechanisms for their effects. Finally, hematoxylin-eosin staining immunohistochemistry animal studies. Results: Our study evaluated antioxidant efficacy BEPs fermented with Lactobacillus plantarum hydrogen peroxide (H2O2)-HDFs UVB-induced skin damage hairless mice. We demonstrated exhibited enhanced properties compared non-fermented (EPs). facilitated H2O2-damaged HDFs, reduced ROS levels, restored mitochondrial membrane potential. suppressed phosphorylation ERK, p38, JNK as mechanism. further dietary supplementation downregulated metalloproteinase 1 (MMP1) expression upregulated I (COL1) UVB-damaged mice, indicating more effective EPs. Conclusions: suggest hold significant potential protective agent mitigating health.

Language: Английский

Citations

0
Identification and Validation of the Potential Key Biomarkers for Atopic Dermatitis Mitochondrion by Learning Algorithms DOI Creative Commons

Junhao Xu,

Xinyu Pan, Miao Zhang

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 4291 - 4306

Published: March 1, 2025

Atopic dermatitis (AD) is a common inflammatory skin condition characterized by erythema and pruritus. Its precise pathogenesis remains unclear, though factors such as genetic predisposition, autoantigen response, allergen exposure, infections, barrier dysfunction are involved. Research suggests correlation between AD mitochondrial dysfunction, well oxidative stress in tissues. Skin sample datasets related to (GSE36842, GSE120721, GSE16161, GSE121212) were retrieved from the GEO database. Differential gene analysis identified differentially expressed genes (DEGs) AD. Three potential biomarkers-COX17, ACOX2, ADH1B-were using LASSO Support Vector Machine (SVM) algorithms. These biomarkers validated through ROC curve analysis, nomogram modeling, calibration curves, real-time PCR. Immune infiltration assessed correlations of biomarkers. Additionally, single-cell GSE153760 dataset nine cell clusters confirmed expression patterns three hub genes. 150 upregulated 367 downregulated Enrichment revealed significant pathways function, stress, energy metabolism samples patients. Area under (AUC) values for COX17, ADH1B 1.000, 0.928, 0.895, respectively, indicating strong predictive capacity. qPCR results showed COX17 was highly lesions, while ACOX2 higher normal skin, consistent with previous findings. Correlation indicated positively correlated resting mast cells but negatively activated T NK cells, positive negative cells. This study that may serve findings could provide insights treatment prognosis conditions.

Language: Английский

Citations

0
Insomnia Associated With Increased Risk of Atopic Dermatitis: A Two‐Sample Mendelian Randomization Study DOI Creative Commons
Xiuqin Ni, Xing Li, Jiayan Li

et al.

Brain and Behavior, Journal Year: 2025, Volume and Issue: 15(5)

Published: May 1, 2025

ABSTRACT Background The causal relationships between sleep traits and allergic diseases remain unclear. This study sought to explore their associations using Mendelian randomization (MR) analysis. Methods utilized summary‐level data from genome‐wide association studies (GWAS) selected genetic variants associated with as instrumental variables (IVs). For the primary analysis, inverse‐variance weighted (IVW) method was utilized. To further evaluate effects, we applied median, mode, MR‐Egger regression. Sensitivity analyses, such linkage disequilibrium score (LDSC) regression, Cochran's Q test, leave‐one‐out MR‐PRESSO, were carried out confirm result robustness. Results IVW analysis revealed that genetically predicted insomnia causally a higher risk of atopic dermatitis (OR = 1.79, 95% CI: 1.17‐2.74, P 0.01), preferring an evening chronotype lower rhinitis (IVW: OR 0.99, 0.99‐1.00, 0.02). LDSC identified significant correlation (r g 0.107, 0.039), but not ‐0.036, 0.339). No connections other diseases. intercept test did indicate pleiotropy, except for asthma. Conclusion Chronotype efficacy sleep‐based interventions in disease management.

Language: Английский

Citations

0
Metallic Nanoparticles and Cosmetics: The Role of Mitochondria and Premature Aging DOI
Veronica Montesinos‐Cruz, Justin Olmanson

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2025, Volume and Issue: 17(3)

Published: May 1, 2025

ABSTRACT The cosmetic industry has developed and commercialized numerous products using new technologies, making them increasingly appealing to the public. use of metallic nanoparticles (MtNPs) as key ingredients in cosmetics become more widespread due their demonstrated benefits. However, these remains controversial, some studies have shown that MtNPs can penetrate deeper layers skin disrupt homeostatic balance. It also been interaction between keratinocytes increases generation reactive oxygen species (ROS), which lead oxidative stress, a condition associated with premature aging. Mitochondria, main organelles responsible for energy production, provide maintain regulatory mechanisms necessary sustain strong protective barrier against environmental threats including MtNPs. To date, relationship redox imbalance, aging, central role mitochondria under investigation fact mitochondrial metabolism, linked increased is development various defense mechanisms, antioxidant enzymes. In this review, we focus on stress generated function enzymes detoxifying metal ions from contained cosmetics. We examine effects potential

Language: Английский

Citations

0
L-Theanine Prolongs the Lifespan by Activating Multiple Molecular Pathways in Ultraviolet C-Exposed Caenorhabditis elegans DOI Creative Commons
Liangwen Chen, Guijie Chen,

Tingting Gai

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2691 - 2691

Published: June 6, 2024

L-theanine, a unique non-protein amino acid, is an important bioactive component of green tea. Previous studies have shown that L-theanine has many potent health benefits, such as anti-anxiety effects, regulation the immune response, relaxing neural tension, and reducing oxidative damage. However, little known concerning whether can improve clearance mitochondrial DNA (mtDNA) damage in organisms. Here, we reported treatment increased ATP production improved morphology to extend lifespan UVC-exposed nematodes. Mechanistic investigations showed enhanced removal mtDNA extended by activating autophagy, mitophagy, dynamics, unfolded protein response (UPRmt) In addition, also upregulated expression genes related energy metabolism Our study provides theoretical basis for possibility tea drinking may prevent mitochondrial-related diseases.

Language: Английский

Citations

2
Tailoring biomaterials for skin anti-aging DOI Creative Commons

Xin Dan,

Songjie Li, Han Chen

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101210 - 101210

Published: Aug. 28, 2024

Skin aging is the phenomenon of degenerative changes in structure and function skin tissues over time manifested by a gradual loss elasticity firmness, an increased number wrinkles, hyperpigmentation. anti-aging refers to reduction through medical cosmetic technologies. In recent years, new biomaterials have been continuously developed for improving appearance mechanical tissue filling, regulating collagen synthesis degradation, inhibiting pigmentation, repairing barrier. This review summarizes mechanisms associated with aging, describes that are commonly used aesthetics their possible modes action, discusses application strategies this area. Moreover, synergistic effects such other active ingredients, as stem cells, exosomes, growth factors, antioxidants, on regeneration evaluated. Finally, challenges development prospects field discussed, novel ideas future innovations area summarized.

Language: Английский

Citations

2
L-theanine Prolongs the Lifespan by Activating Multiple Molecular Pathways in UVC Exposed <em>C. elegans</em> DOI Open Access

Liang wen Chen,

Gui jie Chen,

Ting ting Gai

et al.

Published: April 3, 2024

L-Theanine, a unique non-protein amino acid, is an important bioactive component of green tea. Previous studies have shown that L-theanine has many potent health benefits, such as anti-anxiety effect, regulation immune response, relaxing neural tension, reducing oxidative damage. However, little known concerning whether can improve the clearance mitochondrial DNA (mtDNA) damage in organisms. Here, we reported treatment increased ATP production and improved morphology to extend lifespan UVC-exposed nematodes. Mechanistic investigations showed enhanced removal mtDNA extended by activating autophagy, mitophagy, dynamics, unfolded protein response (UPRmt) In addition, also up-regulated expression genes related energy metabolism Our study provided theoretical basis for possibility tea drinking may prevent diseases.

Language: Английский

Citations

1