Topical Delivery of Artificial Lipidated Antifungal Proteins for the Treatment of Subcutaneous Fungal Infections Using a Biocompatible Ionic Liquid-Based Microemulsion DOI
Muhammad Safaat, Hendra Saputra, Pugoh Santoso

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: 17(2), P. 3062 - 3071

Published: Jan. 6, 2025

The rising incidence of fungal infections, compounded by the emergence severe antifungal resistance, has resulted in an urgent need for innovative therapies. We developed protein-based formulation as a topical agent combining artificial lipidated chitin-binding domain chitinase (LysM-lipid) with recently ionic liquid-in-oil microemulsion formulations (MEFs). Our findings demonstrated that lipid moieties attached to LysM and MEFs effectively disrupted integrity stratum corneum mouse skin model, thereby enhancing permeability LysM-lipids. Among incorporating modified lauric (C12), myristic (C14), palmitic (C16) acids, LysM-C14-loaded MEF emerged most promising candidate, exhibiting potent activity against Trichoderma viride growing actively beneath skin. stability was investigated after 28 day storage period at room temperature, both LysM-C14- LysM-C16-loaded retained comparable freshly prepared MEFs. These results highlight considerable potential LysM-lipid-loaded effective agents.

Language: Английский

Topical Delivery of Artificial Lipidated Antifungal Proteins for the Treatment of Subcutaneous Fungal Infections Using a Biocompatible Ionic Liquid-Based Microemulsion DOI
Muhammad Safaat, Hendra Saputra, Pugoh Santoso

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: 17(2), P. 3062 - 3071

Published: Jan. 6, 2025

The rising incidence of fungal infections, compounded by the emergence severe antifungal resistance, has resulted in an urgent need for innovative therapies. We developed protein-based formulation as a topical agent combining artificial lipidated chitin-binding domain chitinase (LysM-lipid) with recently ionic liquid-in-oil microemulsion formulations (MEFs). Our findings demonstrated that lipid moieties attached to LysM and MEFs effectively disrupted integrity stratum corneum mouse skin model, thereby enhancing permeability LysM-lipids. Among incorporating modified lauric (C12), myristic (C14), palmitic (C16) acids, LysM-C14-loaded MEF emerged most promising candidate, exhibiting potent activity against Trichoderma viride growing actively beneath skin. stability was investigated after 28 day storage period at room temperature, both LysM-C14- LysM-C16-loaded retained comparable freshly prepared MEFs. These results highlight considerable potential LysM-lipid-loaded effective agents.

Language: Английский

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