Ultrastructural Study and Immunohistochemical Characteristics of Mesencephalic Tegmentum in Juvenile Chum Salmon (Oncorhynchus keta) Brain After Acute Traumatic Injury

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 644 - 644

Published: Jan. 14, 2025

The ultrastructural organization of the nuclei tegmental region in juvenile chum salmon (Oncorhynchus keta) was examined using transmission electron microscopy (TEM). dorsal (DTN), nucleus fasciculus longitudinalis medialis (NFLM), and oculomotor nerve (NIII) were studied. examination provided detailed characteristics neurons forming showed neuro-glial relationships them. Neurons three size types with a high metabolic rate, characterized by presence numerous mitochondria, polyribosomes, Golgi apparatus, cytoplasmic inclusions (vacuoles, lipid droplets, dense bodies), distinguished. It found that large interneurons NFLM formed contacts protoplasmic astrocytes. Excitatory synaptic structures identified tegmentum their characteristic are for first time. Microglia-like cells NIII. neurogenic zones also determined In tegmentum, adult-type neural stem progenitor (aNSPCs) corresponding to III IVa Danio rerio. neuroepithelial-like (NECs) previously described from zebrafish cerebellum characterized. salmon, patterns paracrine neurosecretion observed recorded. Patterns apoptosis TEM. Using immunohistochemical (IHC) labeling brain lipid-binding protein (BLBP) aromatase B (AroB), expression intact animals post-traumatic period after acute injury medulla oblongata response brainstem activate multiple signaling pathways, which significantly increases BLBP AroB various regions valvula cerebelli. However, localizations not same. addition general increase parenchyma, overexpression rostro-lateral zone (RLTNZ), while RLTNZ completely absent. Another difference peripheral formation reactive clusters ventro-medial tegmentum. Thus, period, pathways activated whose components putative candidates inducers "astrocyte-like" similar those present mammalian brain. this case, acted as factor enhancing differentiation both radial glia neurons. Estradiol AroB+ astrocytes exerted neuroprotective effects through potential inhibition inflammatory processes. These results indicate new role neuronal aromatization mechanism preventing development neuroinflammation. Moreover, our findings support hypothesis is glial

Language: Английский

KDM2B and its peptides promote the stem cells from apical papilla mediated nerve injury repair in rats by intervening EZH2 function

Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Abstract How to improve the neurogenic potential of mesenchymal stem cells (MSCs) and develop biological agent based on underlying epigenetic mechanism remains a challenge. Here, we investigated effect histone demethylase Lysine (K)‐specific 2B (KDM2B) differentiation nerve injury repair by using MSCs from dental apical papilla (SCAP). We found that KDM2B promoted indicators expression neural spheres formation in SCAP, modified Histone H3K4 trimethylation (H3K4me3) methylation neurogenesis‐related genes. improved SCAP mediated recovery motor ability at early healing stage spinal cord rats. Meanwhile, acted as negative regulator its partner EZH2 during differentiation, enhancer zeste homologue 2 (EZH2) suppressed SCAP. Further, protein interaction between was identified which decreased differentiation. On this basis, revealed seven key binding sequences EZH2, synthesized KDM2B‐peptides these sequences. By usage KDM2B‐peptides, function effectively intervened promoted. More, significantly functional SCI phase. Our study promotor through negatively regulating provided candidate agents for improving repair.

Language: Английский

Differential impact of artificial light at night on cognitive flexibility in visual and spatial reversal learning tasks

Animal Behaviour, Journal Year: 2024, Volume and Issue: 218, P. 173 - 183

Published: Nov. 2, 2024

Language: Английский