Journal of Genetic Engineering and Biotechnology, Journal Year: 2024, Volume and Issue: 22(4), P. 100437 - 100437
Published: Nov. 13, 2024
Language: Английский
Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(8)
Published: July 5, 2024
Language: Английский
Citations
9
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 21, 2025
Potatoes are a critical staple crop worldwide, yet their yield is significantly constrained by salt stress. Understanding and enhancing tolerance in potatoes crucial for ensuring food security. This study evaluated the of 17 diverse potato varieties using principal component analysis, membership function cluster stepwise regression analysis. Comprehensive evaluation based on morphological, physiological, biochemical indicators divided into three categories, identifying Z1264-1, Z700-1, Z943-1, Z1266-1, Z510-1, Z1076-1 as having strong tolerance. Regression equations established stem thickness, root length, catalase activity rapid identification markers tetraploid potatoes. Transcriptome analysis highly tolerant variety identified 68 differentially expressed miRNAs (DE miRNAs). qRT-PCR validation eight randomly selected DE confirmed consistent expression trends with transcriptome data. Predicted target genes these involved calcium channel signaling, osmotic regulation, plant hormone reactive oxygen species clearance. Our findings provide valuable insights screening salt-tolerant germplasms. The also lay foundation studying molecular mechanisms advancing genetic breeding efforts to cultivate more resilient varieties.
Language: Английский
Citations
0
Theoretical and Applied Genetics, Journal Year: 2025, Volume and Issue: 138(3)
Published: Feb. 24, 2025
Language: Английский
Citations
0
Forestry Research, Journal Year: 2025, Volume and Issue: 5(1), P. 0 - 0
Published: Jan. 1, 2025
Soil salinity significantly inhibits plant productivity by adversely affecting photosynthesis and growth. Nitraria sibirica, a typical halophyte, exhibits strong salt tolerance. In this study, salt-treated sibirica seedlings demonstrated more vigorous growth higher photosynthetic rate than untreated control seedlings. Transcriptome analysis revealed that the upregulated differentially expressed genes including ribose 5-phosphate isomerase A, ribulose-bisphosphate carboxylase large chain, malate dehydrogenase in leaves of treated with 500 mM NaCl were enriched 'Carbon fixation organisms' pathway according to Kyoto Encyclopedia Genes Genomes database. The promoters these three predicted contain cis-regulatory elements responsive light, abscisic acid, ethylene. Notably, encoding 1-aminocyclopropane-1-carboxylate synthase, key enzyme ethylene biosynthesis, ethylene-responsive transcription factors under treatment. Furthermore, quantitative real-time PCR confirmed expression was ethephon for 1 h. contrast, salt-induced downregulated μM aminoethoxyvinylglycine, an biosynthesis inhibitor, combination These findings suggest enhanced observed stress is likely mediated signaling, which regulates involved carbon fixation, thereby promoting
Language: Английский
Citations
0
Revista Brasileira de Botânica, Journal Year: 2025, Volume and Issue: 48(1)
Published: March 15, 2025
Language: Английский
Citations
0
BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)
Published: March 27, 2025
The AP2/ERF (APETALA 2/ethylene-responsive element binding factors) is a class of superfamily plant-specific transcription factor that play an important regulatory role in many physiological and biochemical processes plants. In this study, overexpression the StERF79 gene increased drought tolerance potato plants, whereas RNA interference expression (RNAi) lines decreased addition, superoxide dismutase (SOD), peroxide (POD), catalase (CAT) activities, as well proline (Pro) content transgenic lines, showed significantly higher results than those wild type (WT) plants under natural stress conditions, while malondialdehyde (MDA) was lower. can respond to by interacting with DRE cis-acting promoter region downstream target (StDHN-2), activating its expression, result validated using yeast one hybrid (Y1H), Dual-Luciferase β-glucuronidase (GUS) staining assays both vivo vitro. StDHN-2 member dehydrin (DHN) subfamily plant late embryonic developmentally abundant (LEA) protein family. LEA, hydrophilic proteins found serve cellular dewatering protectants prevent desiccation during various stresses. could provide novel knowledge into functional analysis positive regulation response possible mechanisms Not applicable.
Language: Английский
Citations
0
BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 16, 2025
Abstract Background The MAP Kinase cascade system is a conserved signaling mechanism essential for plant development, growth, and stress tolerance. Thus far, genes from the MAPK have been identified in several species but remain uncharacterized polyploid Saccharum spp. Hybrid R570 genome. Results This study 89 ScMAPK , 24 ScMAPKK 107 ScMAPKKK through genome-wide analysis. Phylogenetic classification revealed that four subgroups were present each family, three sub-families (ZIK-like, RAF-like, MEKK-like) presented family. Conserved motif gene structure analysis supported evolutionary relationships of families inferred phylogenetic All mapped on scaffolds (Scaffold_88/89/91/92) nine chromosomes (1–8, 10). Collinearity duplication 169 pairs allelic non-allelic segmentally duplicated genes, contributing to their expansion. Additionally, 13 putative ‘ss-miRNAs’ predicted target 87 with ‘ssp-miR168a’ alone regulating 45 genes. qRT-PCR differential expression under abiotic stressors. ScMAPK07 ScMAPK66 ScRAF43 down-regulated acted as negative regulators. Conversely, ScMAPKK13 ScRAF10 ScZIK18 up-regulated at specific time points drought, exhibiting strong defense. Under NaCl stress, most down-regulated, except slight increases suggesting positive role salt response. CaCl 2 five significantly while remained unchanged, reflecting roles adaptation resource conservation. Conclusion provides insights into evolution function sugarcane, highlighting distinct regulatory responses. Interestingly, some regulators, serving balance responses prevent overactivation. In contrast, others contributed defense mechanisms, offering potential targets resilience improvement. Clinical Trail Number contains no clinical trials. Not applicable.
Language: Английский
Citations
0
Plants, Journal Year: 2024, Volume and Issue: 13(20), P. 2922 - 2922
Published: Oct. 18, 2024
Mitogen-activated protein kinase (MAPK/MPK) cascades are pivotal and highly conserved signaling modules widely distributed in eukaryotes; they play essential roles plant growth development, as well biotic abiotic stress responses. With the development of sequencing technology, complete genome assembly rice without gaps, T2T (Telomere-to-Telomere)-NIP (version AGIS-1.0), has recently been released. In this study, we used bioinformatic approaches to identify analyze MPK kinases (MKKs) based on genome. A total seven OsMKKs were identified, their physical chemical properties, chromosome localization, gene structure, subcellular phylogeny, family evolution, cis-acting elements evaluated. can be divided into four subgroups phylogenetic relationships, members located same evolutionary branch have relatively similar structures domains. Quantitative real-time PCR (qRT-PCR) revealed that all
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4569 - 4569
Published: April 22, 2024
As global climate change continues, drought episodes have become increasingly frequent. Studying plant stress tolerance is urgently needed to ensure food security. The common ice one of the model halophyte plants for biology research. This study aimed investigate functions a newly discovered transcription factor, Homeobox 7 (HB7), from in response stress. An efficient Agrobacterium-mediated transformation method was established plant, where ectopic McHB7 expression may be sustained four weeks. overexpression (OE) displayed tolerance, and activities redox enzymes chlorophyll content OE were higher than wild type. Quantitative proteomics revealed 1910 495 proteins significantly changed leaves compared type under control conditions, respectively. Most increased involved tricarboxylic acid cycle, photosynthesis, glycolysis, pyruvate metabolism, oxidative phosphorylation pathways. Some found participate abscisic signaling or response. Furthermore, levels with bind promoter motifs Early Responsive Dehydration genes acid-responsive genes, protein–protein interaction analysis candidate responsive stresses hormones (e.g., acid). To conclude, contribute enhance through signaling.
Language: Английский
Citations
1
Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15
Published: May 16, 2024
Backgrounds As a conserved signaling pathway, mitogen-activated protein kinase (MAPK) cascade regulates cellular in response to abiotic stress. High temperature may contribute significant decrease economic yield. However, research into the expression patterns of StMAPKK family genes under high is limited and lacks experimental validation regarding their role supporting potato plant growth. Methods To trigger heat stress responses, plants were grown at 35°C. qRT-PCR was conducted analyze pattern plants. Plant with StMAPKK5 loss-of-function gain-of-function developed. Potato growth morphological features assessed through measures height, dry weight, fresh weight. The antioxidant ability indicated by enzyme activity H 2 O content. Cell membrane integrity permeability suggested relative electrical conductivity (REC), contents MDA proline. Photosynthetic capacity next determined. Further, mRNA stress-responsive examined. Results In reaction stress, profiles changed. located nucleus, cytoplasm cytomembrane, playing controlling height weight conditions. over-expression promoted photosynthesis maintained cell integrity, while inhibited transpiration stomatal conductance Overexpression triggered biochemical defenses against modulating levels , proline, as well activities CAT, SOD POD. elicited genetic responses affecting encoding enzymes. Conclusion can improve resilience stress-induced damage, offering promising approach for engineering potatoes enhanced adaptability challenging
Language: Английский
Citations
1