Progress in Characterization of Lipopolysaccharides and Lipid A by Mass Spectrometry

Mass Spectrometry Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT Lipopolysaccharides (LPS) are complex molecules embedded in the outer membrane of Gram‐negative bacteria. LPS highly heterogeneous across species and strains, posing a significant analytical challenge. This review article explores recent advances identification characterization LPS, with particular focus on role mass spectrometry (MS) techniques. The highlights how MS, conjunction various separation methods spanning different ionization techniques dissociation modes, has enabled more precise sensitive determination composition, lipid A structure. Finally, emerging trends MS applications for research discussed its potential to provide deeper insights into development antibiotic resistance among pathogenic

Language: Английский

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LPS binding caspase activation and recruitment domains (CARDs) are bipartite lipid binding modules

Science Advances, Journal Year: 2025, Volume and Issue: 11(10)

Published: March 7, 2025

Caspase-11 is an innate immune pattern recognition receptor (PRR) that detects cytosolic bacterial lipopolysaccharides (LPS) through its caspase activation and recruitment domain (CARD). also eukaryotic (i.e., self) lipids. This observation raises the question of whether common or distinct mechanisms govern interactions with self- nonself-lipids. In this study, using biochemical, computational, cell-based assays, we report caspase-11 CARD functions as a bipartite lipid-binding module. Distinct regions within bind to phosphate groups long acyl chains Self-lipid binding capability conserved across numerous homologs orthologs. The symmetry in nonself-lipid detection enabled us engineer LPS-binding de novo, ancestral CARD-like present fish Amphilophus citrinellus . These findings offer insights into molecular basis LPS by highlight fundamental likely inseparable relationship between self nonself discrimination.

Language: Английский

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Impact on Human Health of Salmonella spp. and Their Lipopolysaccharides: Possible Therapeutic Role and Asymptomatic Presence Consequences

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11868 - 11868

Published: Nov. 5, 2024

Epidemiologically, one of the most important concerns associated with introducing

Language: Английский

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LPS binding caspase activation and recruitment domains (CARDs) are bipartite lipid binding modules

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Abstract Caspase-11 is an innate immune pattern recognition receptor (PRR) that detects cytosolic bacterial lipopolysaccharides (LPS) through its caspase activation and recruitment domain (CARD), triggering inflammatory cell death known as pyroptosis. also eukaryotic ( i.e. self) lipids. This observation raises the question of whether common or distinct mechanisms govern interactions with self nonself In this study, using biochemical, computational, cell-based assays, we report caspase-11 CARD functions a bipartite lipid-binding module. Distinct regions within bind to phosphate groups long acyl chains Self-lipid binding capability conserved across numerous homologs orthologs. The symmetry in lipid detection enabled us engineer LPS-binding de novo , ancestral CARD-like present fish Amphilophus citrinellus . These findings offer critical insights into molecular basis LPS by highlight fundamental likely inseparable relationship between discrimination.

Language: Английский

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The impact of LPS mutants on endotoxin masking in different detection systems

Biologicals, Journal Year: 2024, Volume and Issue: 89, P. 101808 - 101808

Published: Nov. 24, 2024

Language: Английский

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