Transcriptome Profiles Reveal Key Regulatory Networks during Single and Multifactorial Stresses Coupled with Melatonin Treatment in Pitaya (Selenicereus undatus L.) DOI Open Access

Aamir Ali Khokhar,

Hui Liu,

Darya Khan

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8901 - 8901

Published: Aug. 15, 2024

In response to evolving climatic conditions, plants frequently confront multiple abiotic stresses, necessitating robust adaptive mechanisms. This study focuses on the responses of Selenicereus undatus L. both individual stresses (cadmium; Cd, salt; S, and drought; D) their combined applications, with an emphasis evaluating mitigating effects (M) melatonin. Through transcriptome analysis, this identifies significant gene expression changes regulatory network activations. The results show that stress decreases pitaya growth rates by 30%, reduces stem cladode development 40%, increases Cd uptake under single 50% 70%, respectively. Under enhanced activities H2O2, POD, CAT, APX, SOD elevated proline content indicate strong antioxidant defenses. We identified 141 common DEGs related tolerance, most which were AtCBP, ALA, CBP pathways. Interestingly, production genes signal transduction hormones, including abscisic acid auxin, was also significantly induced. Several calcium-dependent protein kinase regulated during M treatments. Functional enrichment analysis showed enriched metabolism, MAPK signaling, photosynthesis. addition, weighted co-expression (WGCNA) critical transcription factors (WRKYs, MYBs, bZIPs, bHLHs, NACs) associated activities, particularly within salmon module. provides morpho-physiological insights into pitaya’s suggests molecular breeding techniques enhance plant resistance.

Language: Английский

Melatonin: Discovery, Biosynthesis, Phytohormones Crosstalk, and Roles in Agricultural Crops under Abiotic Stress Conditions DOI

Parnika Jindal,

Krishan Kant, Navneet Kaur

et al.

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 226, P. 105942 - 105942

Published: Aug. 13, 2024

Language: Английский

Citations

16
Transcriptomic and metabolomic analysis reveals the molecular mechanism of exogenous melatonin improves salt tolerance in eggplants DOI Creative Commons
Handong Wang, Yu Zhang,

Haikun Jiang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Melatonin significantly enhances the tolerance of plants to biotic and abiotic stress, plays an important role in plant resistance salt stress. However, its molecular mechanisms eggplant stress have been rarely reported. In previous studies, we experimentally demonstrated that melatonin can enhance eggplants. this study, treated salt-stressed with a control treatment water, then conducted physiological biochemical tests, transcriptomic metabolomic sequencing, RT-qPCR validation at different stages after treatment. The results showed exogenous alleviate adverse effects on by increasing activity antioxidant enzymes, reducing content reactive oxygen species plants, organic osmoprotectants. Transcriptomic data, as well combined analysis, indicate activate metabolic pathways Compared genes α-linolenic acid metabolism pathway promote accumulation metabolites pathway, significant observed 48 hours treatment, activates expression such SmePLA2, SmeLOXs SmeOPR et al. α-Linolenic acid, (9R,13R)-12-oxophytodienoic 9(S)-HpOTrE (+)-7-iso-Jasmonic acid. validated activating effect candidate a-linolenic pathway. This study analyzed mechanism alleviating providing theoretical foundation for application enhancing production.

Language: Английский

Citations

2
Melatonin-Regulated Chaperone Binding Protein Plays a Key Role in Cadmium Stress Tolerance in Rice, Revealed by the Functional Characterization of a Novel Serotonin N-Acetyltransferase 3 (SNAT3) in Rice DOI Open Access

Hyoung-Yool Lee,

Kyoungwhan Back

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5952 - 5952

Published: May 29, 2024

The study of the mechanisms by which melatonin protects against cadmium (Cd) toxicity in plants is still its infancy, particularly at molecular level. In this study, gene encoding a novel serotonin N-acetyltransferase 3 (SNAT3) rice, pivotal enzyme biosynthetic pathway, was cloned. Rice (Oryza sativa) OsSNAT3 first identified plant ortholog archaeon Thermoplasma volcanium SNAT. purified recombinant catalyzed conversion and 5-methoxytryptamine to N-acetylserotonin melatonin, respectively. suppression RNAi led decline endogenous levels followed reduction Cd tolerance transgenic rice lines. addition, expression genes endoplasmic reticulum (ER) chaperones BiP3, BiP4, BiP5 were much lower lines than wild type. overexpressing (SNAT3-OE), however, higher wild-type plants. SNAT3-OE also tolerated stress, as indicated seedling growth, malondialdehyde, chlorophyll levels. BiP4 These results indicate that engineering could help crops withstand resulting high yields Cd-contaminated fields.

Language: Английский

Citations

4
Influence of Exogenous Melatonin on the Physiological Traits of Camellia hainanica Seedlings Under Polyethylene Glycol-Induced Drought Stress DOI Creative Commons
Liang Ge, Muhammad Zeeshan Ul Haq,

Yanqiang Yao

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 676 - 676

Published: Feb. 22, 2025

This study investigated the effects of exogenous melatonin (MT) on physiological responses Camellia hainanica seedlings under drought stress, using drought-tolerant variety “Hai Da 1” and drought-sensitive “Wan Hai as test materials. Seedlings were treated with MT at concentrations 0, 50, 100, 150, 200, 250 μmol/L through irrigation, followed by stress induced polyethylene glycol (PEG-6000). The results revealed that alleviated growth damage caused PEG-simulated leaf relative conductivity malondialdehyde (MDA) content showing an initial decrease increase concentration rose. In contrast, water content, chlorophyll antioxidant enzyme activity, secondary metabolite levels, carbohydrate initially increased then declined increasing concentration. Treatment 200 notably reduced MDA 40–50%, enhanced activity 20–30%, levels 11–42% in variety. These findings identified optimal for mitigating C. seedlings, providing a foundation its practical application production further research into resistance mechanisms mediated MT.

Language: Английский

Citations

0
The CGA1-SNAT regulatory module potentially contributes to cytokinin-mediated melatonin biosynthesis and drought tolerance in wheat DOI Creative Commons
Roohollah Shamloo‐Dashtpagerdi, Angelica Lindlöf,

Massume Aliakbari

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 7, 2025

Melatonin plays a pivotal role in alleviating abiotic stresses, yet its biosynthesis regulation crops, particularly wheat, remains unclear. This study explores regulatory components of melatonin under drought stress using bioinformatic, physiochemical, and molecular approaches contrasting wheat genotypes. Bioinformatic analysis identified SNAT, key gene, 88 transcription factors (TFs) from 26 families as potential regulators. The network for SNAT highlighted CYTOKININ-RESPONSIVE GATA FACTOR 1 (CGA1) significant TF. Under stress, genotypes exhibited distinct CGA1-SNAT module expression, cytokinin levels, photosynthetic activity, oxidative damage. Cytokinin treatments regulated the module, altering content, SPAD values, chloroplast numbers, drought-susceptible uncovers interaction with pathway regulating during stress. These findings enhance our understanding mechanisms underpinning tolerance offer promising targets genetic biochemical interventions to improve crop resilience.

Language: Английский

Citations

0
Molecular Mechanisms of Plant Abiotic Stress Tolerance DOI Open Access
Michael Moustakas

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2731 - 2731

Published: March 18, 2025

Global climate change compromises sufficient food production, and it is estimated that may be reduced by 11–25% at the end of this century [...]

Language: Английский

Citations

0
Effects of exogenous melatonin on growth and photosynthesis of maize (Zea mays L. ) seedlings under low nitrogen stress DOI
Xianliang Lv, Qing Zhang, Jian He

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 223, P. 109810 - 109810

Published: March 20, 2025

Language: Английский

Citations

0
Melatonin-Induced Chromium Tolerance Requires Hydrogen Sulfide Signaling in Maize DOI Creative Commons
Xiaoxiao Yang, Qifeng Shi, Xinru Wang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1763 - 1763

Published: June 26, 2024

Both melatonin and hydrogen sulfide (H2S) mitigate chromium (Cr) toxicity in plants, but the specific interaction between H2S Cr detoxification remains unclear. In this study, was elucidated by measuring cell wall polysaccharide metabolism antioxidant enzyme activity maize. The findings revealed that exposure to stress (100 μM K2Cr2O7) resulted upregulation of L-/D-cysteine desulfhydrase (LCD/DCD) gene expression, leading a 77.8% 27.3% increase endogenous levels maize leaves roots, respectively. Similarly, system is activated response stress. We found had significant impact on relative expression LCD/DCD, 103.3% 116.7% contrast, NaHS minimal effects mRNA serotonin-Nacetyltransferase (SNAT) levels. production induced accompanied an tolerance, as evidenced elevated content, increased pectin methylesterase activity, improved activity. scavenging decreases melatonin-induced while inhibitor synthesis, p-chlorophenylalanine (p-CPA), has H2S-induced tolerance. conclusion, our suggest serves downstream signaling molecule involved tolerance

Language: Английский

Citations

3
Salicylic Acid and Melatonin Synergy Enhances Boron Toxicity Tolerance via AsA–GSH Cycle and Glyoxalase System Regulation in Fragrant Rice DOI Creative Commons
Muhammad Imran, Émilie Widemann, Sarfraz Shafiq

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(10), P. 520 - 520

Published: Sept. 26, 2024

Boron is an essential micronutrient for plant growth and productivity, yet excessive boron leads to toxicity, posing significant challenges agriculture. Fragrant rice popular among consumers, but the impact of toxicity on qualitative traits fragrant rice, especially aroma, remains largely unexplored. The individual potentials melatonin salicylic acid in reducing are less known, while their synergistic effects mechanisms remain unclear.

Language: Английский

Citations

1
Melatonin: dual players mitigating drought-induced stress in tomatoes via modulation of phytohormones and antioxidant signaling cascades DOI Creative Commons
Shifa Shaffique, Anis Ali Shah,

⋅Sang-Mo Kang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 20, 2024

Drought stress significantly retards the plant production. Melatonin is a vital hormone, signaling molecule, and bio-regulator of diverse physiological growth development processes. Its role in boosting agronomic traits under conditions has received considerable attention. However, underlying molecular mechanism action how they increase drought tolerance not been fully interpreted. The current study aimed to ascertain protective melatonin fortifying antioxidant defense system, modulating phytohormone profile, improving tomato seedlings stress. After V1 stage (1st leaf emerged), were exposed PEG-6000 mimic drought-induced (DR 10% DR 20%), followed by exogenous application 100 µM soil drench. Drought-induced negatively impacted reducing biomass accumulation, diminishing salicylic acid (SA) chlorophyll levels, dramatically lowering ability. protected them activating which decreased oxidative burst increased activities SOD, CAT, APX. Administration drench most remarkably downregulated transcription factors SlDREB3 SlNCED3. This validated moderating potential against maintaining development, enhancing hormone elevating enzyme activities, suppressing relative expression drought-responsive genes. These findings also provide basis for use MT agricultural research other relevant fields study.

Language: Английский

Citations

1