Molecular Characterization of Odorant-Binding Protein Genes Associated with Host-Seeking Behavior in Oides leucomelaena DOI Open Access
Ning Zhao,

Kai Li,

Huifen Ma

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9436 - 9436

Published: Aug. 30, 2024

The identification of odorant-binding proteins (OBPs) involved in host location by Oides leucomelaena (O. Weise, 1922, Coleoptera, Galerucinae) is significant for its biological control. Tools the NCBI database were used to compare and analyze transcriptome sequences O. with OBP other chemosensory-related Coleoptera insects. Subsequently, MEGA7 was utilized sequence alignment construction a phylogenetic tree, combined expression profiling screen candidate antennae-specific OBPs. In addition, fumigation experiments star anise volatiles conducted assess antennae specificity Finally, molecular docking employed speculate on binding potential OBPs volatiles. study identified 42 OBPs, 8 chemosensory 27 receptors. OleuOBP3, OleuOBP5, OleuOBP6 as classic family members specific antennae, which confirmed volatile experiments. Molecular ultimately clarified that all exhibit high affinity β-caryophyllene among We successfully obtained three from determined their high-affinity volatiles, providing theoretical basis development attractants subsequent stages.

Language: Английский

Molecular Characterization of Odorant-Binding Protein Genes Associated with Host-Seeking Behavior in Oides leucomelaena DOI Open Access
Ning Zhao,

Kai Li,

Huifen Ma

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9436 - 9436

Published: Aug. 30, 2024

The identification of odorant-binding proteins (OBPs) involved in host location by Oides leucomelaena (O. Weise, 1922, Coleoptera, Galerucinae) is significant for its biological control. Tools the NCBI database were used to compare and analyze transcriptome sequences O. with OBP other chemosensory-related Coleoptera insects. Subsequently, MEGA7 was utilized sequence alignment construction a phylogenetic tree, combined expression profiling screen candidate antennae-specific OBPs. In addition, fumigation experiments star anise volatiles conducted assess antennae specificity Finally, molecular docking employed speculate on binding potential OBPs volatiles. study identified 42 OBPs, 8 chemosensory 27 receptors. OleuOBP3, OleuOBP5, OleuOBP6 as classic family members specific antennae, which confirmed volatile experiments. Molecular ultimately clarified that all exhibit high affinity β-caryophyllene among We successfully obtained three from determined their high-affinity volatiles, providing theoretical basis development attractants subsequent stages.

Language: Английский

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