Applied Sciences,
Journal Year:
2024,
Volume and Issue:
14(21), P. 9790 - 9790
Published: Oct. 26, 2024
The
enzyme-assisted
approaches
for
plant
phenolics
extraction
are
more
eco-friendly
methods
compared
to
acid
or
alkaline
hydrolysis.
Carbohydrase
enzymes
can
release
free
from
materials
by
cleaving
the
glycosidic
bonds
between
phenolic
compounds
and
cell
wall
polymers.
In
this
study,
efficiency
of
carbohydrase-assisted
treatment
was
evaluated
extract
bioactive
hawthorn
(Crataegus
orientalis)
fruit
residues.
Enzymatic
fruits
operated
using
a
crude
cellulolytic
enzyme
cocktail
Rhizomucor
miehei
NRRL
5282
pectinase
preparate
Aspergillus
niger.
Both
cellulase
combined
cellulase–pectinase
treatments
improved
total
content
(TPC)
antioxidant
activity
extracts.
TPC
increased
1899
±
27
mg
gallic
equivalents/100
g
dry
matter
during
treatment,
showing
strong
correlation
with
average
capacity
determined
ferric-reducing
power
(1.7-fold
increment)
2,2-diphenyl-1-picrylhydrazyl
(1.15-fold
reagents.
major
in
enzyme-treated
extracts
were
vanillic
ferulic
acids,
concentrations
which
115.6-fold
93.9-fold,
respectively,
carbohydrase
treatment.
planktonic
growth
Escherichia
coli,
Bacillus
subtilis,
Pseudomonas
aeruginosa,
Chromobacterium
violaceum
slightly
inhibited
minimum
inhibitory
concentration
values
15.0
77.9
mg/mL,
while
yeasts
tested
quite
resistant
samples.
B.
subtilis
yeast
biofilms
sensitive
extracts,
also
showed
quorum-sensing
effects
against
C.
violaceum.
obtained
hold
potential
as
natural
source
antioxidants
antimicrobials.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(11), P. 5638 - 5638
Published: May 22, 2024
This
study
aimed
to
investigate
the
availability
of
flavonoids,
anthocyanins,
and
phenolic
acids
in
mutant
bean
seeds,
focusing
on
M7
lines,
their
corresponding
initial
local
cultivars.
HPLC-DAD-MS/MS
HPLC-MS/MS
were
used
analyze
twenty-eight
genotypes
common
bean.
The
obtained
results
suggest
that
mutations
resulted
four
newly
synthesized
anthocyanins
namely,
delphinidin
3-O-glucoside,
cyanidin
pelargonidin
petunidin
20
accessions
with
colored
seed
shapes
out
total
28.
Importantly,
cultivar
white
as
well
did
not
contain
anthocyanins.
lines
classified
into
groups
based
colors
novel
qualitative
characteristics.
Five
further
quantified:
ferulic,
p-coumaric,
caffeic,
sinapic,
traces
chlorogenic
acids.
Flavonoids
represented
by
epicatechin,
quercetin,
luteolin,
concentrations
several-fold
superior
compared
those
cultivar.
All
exhibited
higher
flavonoids.
These
findings
contribute
understanding
genetics
biochemistry
accumulation
anthocyanin
production
which
is
relevant
health
benefits
might
have
implications
for
breeding
programs
food
security
efforts.
Foods,
Journal Year:
2024,
Volume and Issue:
13(17), P. 2794 - 2794
Published: Sept. 2, 2024
Sour
and
sweet
cherries
were
evaluated
as
functional
components
in
bread-making
because
of
their
bioactive
microconstituent
content.
Five
forms
enrichment
for
each
fruit,
including
the
hydroalcoholic
extract,
lyophilized
pulverized
combinations,
used
supplementation.
The
physicochemical
(pH,
color,
moisture,
rheology,
texture)
sensory
properties
dough
bread
assessed
different
environments
(biological
chemical
leavening).
cherry
extract
showed
higher
phenolic
content
than
cherry,
especially
form.
viscoelasticity
doughs
varied
based
on
proofing
environment
fortification
Chemically
leavened
exhibited
moduli
(G′,
G″),
complex
viscosity
(η*),
hardness.
Biologically
had
a
lower
pH,
influencing
swelling
percentage,
which
is
linked
to
form
Extract-fortified
displayed
increased
G′,
η*,
hardness
compared
control,
whereas
yeast-leavened
reduced
ability.
Physicochemical
changes
more
significant
systems,
also
scored
evaluations.
Supplementing
bakery
products
with
fruit
enhances
antioxidant
status,
but
conditions
significantly
affect
product.
Applied Sciences,
Journal Year:
2024,
Volume and Issue:
14(21), P. 9790 - 9790
Published: Oct. 26, 2024
The
enzyme-assisted
approaches
for
plant
phenolics
extraction
are
more
eco-friendly
methods
compared
to
acid
or
alkaline
hydrolysis.
Carbohydrase
enzymes
can
release
free
from
materials
by
cleaving
the
glycosidic
bonds
between
phenolic
compounds
and
cell
wall
polymers.
In
this
study,
efficiency
of
carbohydrase-assisted
treatment
was
evaluated
extract
bioactive
hawthorn
(Crataegus
orientalis)
fruit
residues.
Enzymatic
fruits
operated
using
a
crude
cellulolytic
enzyme
cocktail
Rhizomucor
miehei
NRRL
5282
pectinase
preparate
Aspergillus
niger.
Both
cellulase
combined
cellulase–pectinase
treatments
improved
total
content
(TPC)
antioxidant
activity
extracts.
TPC
increased
1899
±
27
mg
gallic
equivalents/100
g
dry
matter
during
treatment,
showing
strong
correlation
with
average
capacity
determined
ferric-reducing
power
(1.7-fold
increment)
2,2-diphenyl-1-picrylhydrazyl
(1.15-fold
reagents.
major
in
enzyme-treated
extracts
were
vanillic
ferulic
acids,
concentrations
which
115.6-fold
93.9-fold,
respectively,
carbohydrase
treatment.
planktonic
growth
Escherichia
coli,
Bacillus
subtilis,
Pseudomonas
aeruginosa,
Chromobacterium
violaceum
slightly
inhibited
minimum
inhibitory
concentration
values
15.0
77.9
mg/mL,
while
yeasts
tested
quite
resistant
samples.
B.
subtilis
yeast
biofilms
sensitive
extracts,
also
showed
quorum-sensing
effects
against
C.
violaceum.
obtained
hold
potential
as
natural
source
antioxidants
antimicrobials.
