Experimental PTSD Models in Zebrafish: A Systematic Review of Behavioral, Neurochemical, and Molecular Outcomes

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 456 - 456

Published: April 23, 2025

Post-traumatic stress disorder (PTSD) is a complex psychiatric condition characterized by persistent behavioral and neurobiological alterations following trauma. Although rodent models are commonly used to study PTSD, zebrafish (Danio rerio) have emerged as promising alternative due their genetic similarity humans, conserved response systems, high-throughput capabilities. This systematic review evaluates 33 experimental studies on PTSD models, focusing behavioral, neurochemical, molecular outcomes. Chronic unpredictable (CUS/UCS) paradigms of 14–15 days were identified the most reliable for inducing PTSD-like phenotypes, consistently resulting in anxiety-like behaviors, cortisol dysregulation, gene expression changes. In contrast, acute produced transient effects, social defeat showed methodological variability. frequently demonstrated neurotransmitter imbalances, oxidative stress, upregulation inflammatory neuroplasticity-related genes. However, literature revealed challenges, including protocol heterogeneity, limited sex-specific analyses, constraints longitudinal biomarker tracking. Future directions include epigenetic profiling, environmental standardization, cross-species validation. When with rigor, offer powerful translationally relevant platform mechanisms screen novel interventions.

Language: Английский

Quantifying Variability in Zebrafish Larvae Locomotor Behavior across Experimental Conditions: A Learning-Based Tracker

Fishes, Journal Year: 2024, Volume and Issue: 9(6), P. 193 - 193

Published: May 23, 2024

This study investigated the effects of environmental changes on zebrafish larval behavior, using single-factor and orthogonal experiments to assess locomotion during temperature pH changes. In experiments, larvae were exposed variations in (22 30 °C) levels (6.0, 7.0, 9.0). The simultaneous by tests. both each larva was recorded three 5 min videos at different stages (before exposure, short-term exposure (10 min), after long-term (60 min)). You Look Only Once (YOLOv5) Deep Simple Online Real Time Tracking (DeepSORT) models adopted develop a tracking system, YOLOv5 improved two aspects anchor clustering network structure. accuracy system for small targets effectively improved, reaching more than 98% MOTA (Multiple Object Accuracy). Principal Component Analysis (PCA) employed extract behavioral features from 13 motion parameters, namely activity, edge direction preference. Our findings reveal that lower temperatures acidic conditions led decrease former also increased behavior. Conversely, elevated alkaline had muted impact these behaviors. Interestingly, concurrent significantly altered directional Additionally, we observed elicited distinct temporal patterns constant level. summary, recommend precise control explicit reporting ambient breeding devices experimental wells minimize behavior enhance experiment repeatability reliability.

Language: Английский

Improving Automated Behaviour Analysis in Zebrafish Laboratory Trials

Acta Physica Polonica A, Journal Year: 2024, Volume and Issue: 146(4), P. 483 - 489

Published: Oct. 1, 2024

Language: Английский