Human miR-1 Stimulates Metabolic and Thermogenic-Related Genes in Adipocytes DOI Open Access

Ester Díez-Sainz,

Fermı́n I. Milagro, Paula Aranaz

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 276 - 276

Published: Dec. 31, 2024

MicroRNAs play a pivotal role in the regulation of adipose tissue function and have emerged as promising therapeutic candidates for management obesity associated comorbidities. Among them, miR-1 could be potential biomarker metabolic diseases contribute to homeostasis. However, thorough research is required fully elucidate impact on human adipocyte thermogenesis metabolism. This study aimed explore effect browning, process whose activation has been linked protection counteraction. Human multipotent adipose-derived stem cells, hMADS were differentiated into white brown-like adipocytes transfected with mimics gene expression western blotting analyses. inhibited its previously validated target PTK9/TWF1 modulated profile key genes involved browning (increased UCP1 at mRNA protein level, increased CPT1M, decreased HIF3A), differentiation metabolism (decreased PLIN1, FASN, RXRA, PPARG, FABP4, MAPKAPK2), well related cytoskeleton ACTB) extracellular matrix COL1A1). These findings suggest that can modulate metabolism, which hold value eventual obesity.

Language: Английский

Weighing in on the role of brown adipose tissue for treatment of obesity DOI Creative Commons

Brinda Prapaharan,

Micah Lea,

Jacqueline L. Beaudry

et al.

Journal of Pharmacy & Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 27

Published: July 17, 2024

Brown adipose tissue (BAT) activation is an emerging target for obesity treatments due to its thermogenic properties stemming from ability shuttle energy through uncoupling protein 1 (Ucp1). Recent rodent studies show how BAT and white (WAT) activity can be modulated increase the expression of proteins. Consequently, these alterations enable organisms endure cold-temperatures elevate expenditure, thereby promoting weight loss. In humans, less abundant in obese subjects impacts thermogenesis are pronounced, bringing into question whether expending seen rodents translated human models. Our review will discuss pharmacological, hormonal, bioactive, sex-specific environmental activators inhibitors determine potential act as a therapeutic strategy. We aim address feasibility utilizing modulators reduction individuals, recent suggest that BAT’s contributions expenditure along with Ucp1-dependent -independent pathways may or not rectify imbalance characteristic obesity.

Language: Английский

Citations

8
Human miR-1 Stimulates Metabolic and Thermogenic-Related Genes in Adipocytes DOI Open Access

Ester Díez-Sainz,

Fermı́n I. Milagro, Paula Aranaz

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 276 - 276

Published: Dec. 31, 2024

MicroRNAs play a pivotal role in the regulation of adipose tissue function and have emerged as promising therapeutic candidates for management obesity associated comorbidities. Among them, miR-1 could be potential biomarker metabolic diseases contribute to homeostasis. However, thorough research is required fully elucidate impact on human adipocyte thermogenesis metabolism. This study aimed explore effect browning, process whose activation has been linked protection counteraction. Human multipotent adipose-derived stem cells, hMADS were differentiated into white brown-like adipocytes transfected with mimics gene expression western blotting analyses. inhibited its previously validated target PTK9/TWF1 modulated profile key genes involved browning (increased UCP1 at mRNA protein level, increased CPT1M, decreased HIF3A), differentiation metabolism (decreased PLIN1, FASN, RXRA, PPARG, FABP4, MAPKAPK2), well related cytoskeleton ACTB) extracellular matrix COL1A1). These findings suggest that can modulate metabolism, which hold value eventual obesity.

Language: Английский

Citations

1